TRADD regulates perinatal development and adulthood survival in mice lacking RIPK1 and RIPK3.

TRADD is an adaptor for TNFR1-induced apoptosis and NFκB activation. However, TRADD-deficient mice undergo normal development and contain normal lymphoid populations, which contrasts with an embryonic defect in mice lacking FADD, the shared adaptor mediating apoptosis. Recent studies indicate FADD suppresses embryonic necroptosis mediated by RIPK1. TRADD was suggested to also mediate necroptosis. Here we report that targeting TRADD fails to rescue Fadd-/- embryos from necroptosis, and ablation of TRADD rescues Ripk1-/- mice from perinatal lethality when RIPK3-mediated necroptosis is disabled. The resulting Ripk1-/-Ripk3-/-Tradd-/- mice survive until early adulthood, but die thereafter. A single allele of Tradd is optimal for survival of Ripk1-/-Ripk3-/-Tradd+/- mice. We show that TRADD plays a more dominating role in NFκB-signaling than RIPK1. While RIPK1 protects thymocytes from TNFα-induced apoptosis, TRADD promotes this process. The data demonstrate that TRADD is critical in perinatal and adult mice lacking RIPK1 and RIPK3, which has not been appreciated in prior studies.

Introduction

Programmed cell death (PCD) including apoptosis and necroptosis plays an important role during development[1,2]. In the immune system, PCD is required for homeostasis and suppression of autoimmunity[3,4]. Signaling through death receptors (DRs) of the TNFR1/Fas family can lead to PCD[5]. Apoptosis is mediated by the Fas associated death domain (FADD) adaptor protein, which recruits and activates caspase 8 to trigger the apoptotic program[6-11]. Apoptotic cells are engulfed by phagocytic cells, preventing spillage of intracellular contents, and thus limiting tissue damage and inflammation[12-14]. When apoptosis is blocked, necroptosis (or programmed necrosis) is initiated by receptor interacting protein kinase (RIPK)1 and RIPK3[15-19]. These two protein serine/threonine kinases interact with one another via their RIP homotypic interaction motif (RHIM). This results in phosphorylation of both RIPK1 and RIPK3 and recruitment/activation of the mixed lineage kinase domain like (MLKL) protein. Activated MLKL translocates to and disrupts the plasma membrane[20,21]. Loss of membrane integrity during necroptosis results in the release of cellular contents, leading to inflammatory responses[22].

Although FADD and Caspase 8 were initially characterized as mediators of apoptotic cell death, genetic studies demonstrated these proteins paradoxically play a major role in preventing necroptotic cell death. Fadd embryos and Casp8 embryos die in utero and do not survive past embryonic day (E)10.5[23-25]. Deletion of RIPK1 or RIPK3 corrects the embryonic defect in Fadd or Casp8 mice[26-28]. Furthermore, deletion of RIPK3 completely restores normal development of Fadd or Casp8 mice[27-29]. The resulting Casp8Ripk3 or FaddRipk3 double knockout (DKO) mice develop progressive lymphadenopathy and splenomegaly, a hallmark of lymphoproliferative (lpr) diseases.

Although RIPK1 (RIP or RIP1) was originally cloned as a Fas-interacting protein, deletion of RIPK1 results in perinatal lethality[30], while Fas is dispensable in mouse development. Moreover, conditional deletion of RIPK1 specifically in T cells does not affect Fas-induced apoptosis[31]. Ripk1 mice survive into late adulthood only when apoptosis is blocked by ablation of FADD or Caspase 8 and necroptosis is blocked by ablation of RIPK3[29,32-34]. When only necroptosis is blocked, Ripk1Ripk3 mice die within several days after birth. TRADD is the primary adaptor for TNFR1 and can induce both apoptosis and NFκB activation[35,36]. However, Tradd mice are viable and show no apparent developmental abnormality, despite defective TNFR1-mediated apoptosis and NFκB and MAPK signaling in Tradd cells[37-39]. TRADD also mediates apoptosis and activation of NFκB and MAPK signaling through toll-like receptor (TLR)3 and TLR4 as well as other DRs[38,40]. It has been suggested that TRADD, like RIPK1, can mediate necroptosis[38]. A better understanding of how TRADD fits into current models of cell death induction and regulation is essential to uncover a more complete picture of apoptotic and necroptotic signaling.

Here, we report an approach to investigate the physiological function of TRADD by employing unique animal models. The data indicates that TRADD plays no role in embryonic necroptosis in Fadd mice. However, the study uncovers a critical function for TRADD in mouse perinatal development as well as in adult mouse survival in the absence of RIPK1 and RIPK3. Moreover, TRADD is essential for lymphocyte survival in adult mice. The data show that TRADD mediates both apoptosis and NFκB activation. A single allele of TRADD is optimal for survival of of Ripk1Ripk3 mice, indicating a Goldilocks principle.

Results

TRADD is dispensable for necroptosis in mouse embryos

In agreement with previous analysis of Casp8 mice[33], we found that lack of TNFα improves the survival of Fadd embryos (Supplementary Fig. 1), indicating that TNFR1-mediated necroptosis is partially responsible for embryonic death. TRADD is suggested to be the primary adaptor for TNFR1 and likely mediates both apoptosis and NFκB activation[35,36]. However, Tradd mice exhibit no apparent defect in development and contain a normal hematopoietic compartment[37-39]. TRADD was also suggested to mediate TNFα-induced necroptosis[38]. However, we found that absence of TRADD failed to rescue Fadd embryos from death (Supplementary Fig. 2).

Ripk1 or Ripk1Fadd mice undergo normal embryogenesis but die within one day of birth due to RIPK3-mediated necroptosis[26,30]. In contrast, Ripk1FaddRipk3 mice are viable, indicating that RIPK3-mediated necroptosis is the leading cause of perinatal lethality in Ripk1Fadd mice[29,32-34]. Given possible functional redundancy between TRADD and FADD for apoptotic signaling and potential functional redundancy between TRADD and RIPK1 for NFκB signaling, we analyzed the impact of TRADD inactivation on the survival of Ripk1 or Ripk1Fadd mice. As shown in Supplementary Fig. 3, all Ripk1Tradd mice died within one day of birth and were indistinguishable from Ripk1 mice, indicating that deletion of TRADD has no survival benefit in Ripk1 mice. In addition, intercross of Ripk1FaddTradd mice yielded no viable Ripk1FaddTradd mice at weaning age (Supplementary Fig. 3a, c), unlike the normal postnatal development seen in Ripk1Ripk3Fadd mice described in prior studies[29,32-34]. In total, the data indicate that TRADD does not mediate lethal necroptosis in Fadd embryos or in Ripk1 and Ripk1Fadd neonates.

TRADD deletion in Ripk1Ripk3 mice

We performed further analysis to evaluate the in vivo function of TRADD in apoptosis using Ripk1Ripk3 mice, in which necroptosis is blocked. To this end, viable Ripk1Ripk3Tradd mice were generated and intercrossed. Weaning age Ripk1Ripk3Tradd triple knockout (TKO) mice were detected at expected Mendelian frequencies (Fig. 1a), which is in sharp contrast to the perinatal lethality exhibited by Ripk1Ripk3 neonates (Fig. 1b). This data provides in vivo evidence that a TRADD-mediated signaling pathway blocks perinatal mouse development in the absence of RIPK1 and RIPK3. However, Ripk1Ripk3Tradd mice fail to thrive in adulthood and progressively lose weight over a period of several weeks (Fig. 1b–d). Histological analysis of the Ripk1Ripk3Tradd large intestine showed an obvious loss of goblet cells and massive immune cell infiltration in the TKO intestine (Fig. 1e). In addition, elevated levels of activated caspase 3 (CC3) were evident in the large intestine of the Ripk1Ripk3Tradd TKO mice, when compared to wild-type control mice (Fig. 1e). Among the mice analyzed, the longest survival is postnatal day (P)78, when the moribund animal was euthanized (Fig. 1b).

Fig. 1

Deletion of TRADD improves the survival of Ripk1Ripk3 into young adulthood. a Actual and expected frequencies of pups at weaning age from intercrosses of Ripk1Ripk3 Tradd mice. Chi square analysis shows no significant difference between actual and expected (p = 0.472). b Kaplan–Meier plot showing survival of mice of indicated genotypes. Logrank tests shows a significant increase in survival of Ripk1 Ripk3 Tradd mice, compared to Ripk1 Ripk3 mice (p < 0.0001) but significantly less than WT mice (p < 0.0001). c Weights of Ripk1 Ripk3 Tradd mice and littermate controls over time presented as mean ± SEM, *p < 0.05 as determined by Student’s t test. d Three week old and two month old Ripk1 Ripk3 Tradd mice (*) with littermate controls (n = 5 independent experiments). e Hematoxylin and eosin (H&E) and cleaved caspase 3 (CC3) staining of the large intestine of indicated genotypes (n = 3 independent experiments). Scale bars are 50 μm

To understand the role of TRADD in adult mice further, analysis was performed with intercross of triple heterozygous (THZ) Ripk1Ripk3Tradd mutant mice. Interestingly, heterozygosity of both RIPK3 and TRADD was sufficient for Ripk1 mice to survive from birth to weaning age (Fig. 2a). Among the resulting Ripk1Ripk3Tradd mutant mice analyzed, survival up to P85 was observed, when the moribund animal was euthanized (Fig. 2b). Remarkably, retaining just one wild-type allele of the Tradd gene provides better survival than two wild-type alleles (Tradd+/+) or two KO alleles (Tradd) for adult Ripk1Ripk3 mice (Fig. 2a–c). Weaning age Ripk1Ripk3Tradd mice are found at expected Mendelian frequencies (Fig. 2a) and survived normally throughout adulthood (Fig. 2b), in contrast to Ripk1Ripk3Tradd mice, which die in early adulthood (Fig. 1b). Ripk1Ripk3Tradd mice are indistinguishable in appearance and weight from wild-type controls (Fig. 2c, d), and are fertile. In addition, Ripk1Ripk3Tradd mice were similar to wild-type mice with minimal levels of activated caspase 3 in the large intestine (Fig. 2e), unlike Ripk1Ripk3Tradd mice, which show greatly elevated active caspase 3 levels in the large intestine (Fig. 1e). Ripk1Ripk3Tradd mice older than 6 months display no enlargement of the lymphoid organs (Supplementary Fig. 4a) nor presence of the abnormal CD3+B220+ population (Supplementary Fig. 4b), the typical symptoms of lymphoproliferative (lpr) disease present in Fas mice. This contrasts the severe lpr phenotype in FaddRipk3 or FaddRipk3Ripk1 mice shown previously by others and us (Supplementary Fig. 4)[29,32-34].

Fig. 2

Haplosufficiency of TRADD rescues the lethality of Ripk1Ripk3 mice. a Expected and actual frequencies of pups at weaning age from a triple heterozygous cross of Ripk1Ripk3Tradd mice. b Kaplan-Meier plot showing survival of mice of indicated genotypes. Logrank tests showed Ripk1Ripk3Tradd mice exhibited improved survival over Ripk1Ripk3Tradd and Ripk1Ripk3Tradd mice (p < 0.0001). c One month old mice of indicated genotypes (n = 5 independent experiments). d Weights of Ripk1Ripk3Tradd mice and littermate controls over time presented as mean ± SEM. t test shows no significant difference between groups. e H&E and cleaved caspase 3 (CC3) staining of the large intestine of indicated genotypes (n = 3 independent experiments). Scale bars are 50 μm

A T cell defect in adult Ripk1Ripk3Tradd mice

Tradd mice contain normal lymphocyte populations, indicating a dispensable role for TRADD in lymphopoiesis[38]. Here, we show that in young adult mice (7–8 week old), the lymphoid organs of Ripk1Ripk3Tradd mice are indistinguishable from wild-type controls despite a reduction of TRADD protein levels and the absence of RIPK1 and RIPK3 (Supplementary Fig. 5). However, deletion of both TRADD alleles results in an enlarged spleen in Ripk1Ripk3Tradd mice (Supplementary Fig. 5b, c). Flow cytometric analysis indicates that the spleen of Ripk1Ripk3Tradd mice contain an increased Mac+Gr1+ myeloid cell population (Supplementary Fig. 6,a). The thymus is significantly reduced by size and weight (Supplementary Fig. 5b, c) and thymic cellularity was also greatly decreased compared with WT controls (Supplementary Fig. 5d). Flow cytometric analysis showed a major reduction of the immature CD4+CD8+ double positive population in the thymus of Ripk1Ripk3Tradd mice (Fig. 3a), indicating impaired T cell development. Further analysis of the peripheral lymphoid organs showed that the spleen and lymph nodes exhibited a marked reduction of CD3+ T cell numbers of both CD4+ and CD8+ lineages (Fig. 3a–c), resulting in a dramatic T lymphopenia. In addition, Ripk1Ripk3Tradd mice have significantly reduced bone marrow cell numbers (Supplementary Fig. 5d). Flow cytometric analysis of the B lineage showed a decrease in B220loIgM− pro/pre B cells in the bone marrow and no apparent defect in the periphery in Ripk1Ripk3Tradd mice (Supplementary Fig. 6b, c).

Fig. 3

Ripk1Ripk3Tradd mice display impaired T cell development and T lymphopenia in young adulthood. a, b Representative two-color flow cytometric plots showing a CD4+ and CD8+ T cells in the lymph nodes, spleen, and thymus and b T cells (CD3+) and B cells (B220+) in the lymph nodes and spleen (n = 8 independent experiments). c CD3+ T cell numbers in the lymphoid organs of 7–8 week old mice of indicated genotypes (n = 7 independent experiments). All graphs presented as mean ± SEM

TRADD mediates apoptosis in T cells

Given the pronounced defect in T cell development in the thymus and T-lymphopenia in the periphery in Ripk1Ripk3Tradd mice, we performed analysis of thymocytes for their ability to respond to stimuli through the DRs. WT, Ripk1Ripk3Tradd, and Ripk1Ripk3Tradd thymocytes were similarly sensitive to Fas-induced cell death (Supplementary Fig. 7a). By contrast, Ripk1Ripk3Tradd thymocytes readily undergo TNFα-induced cell death (Fig. 4a). However, wild-type and Ripk1Ripk3Tradd thymocytes were equally resistant to TNFα. This is also true of mature peripheral T cells from the genotypes tested, which all undergo Fas-mediated apoptosis but only Ripk1Ripk3Tradd cells are sensitive to TNFα-induced cell death (Supplementary Fig. 7b, c). Ripk1Ripk3Tradd thymocytes are also sensitive to cell death induced by human TNFα, which binds to TNFR1 and not TNFR2[41] (Supplementary Fig. 7d). In addition, blocking of TNFR1, but not TNFR2, largely prevents this cell death (Supplementary Fig. 7e). These data indicate that lack of RIPK1 sensitizes thymocytes to TRADD-mediated apoptosis induced by TNFα through TNFR1.

Fig. 4

Cell death and survival of Ripk1Ripk3Tradd and Ripk1Ripk3Tradd cells. a Thymocytes were treated with indicated concentrations of TNFα for 16 h with 30 μg/mL cycloheximide. After addition of 1 μg/mL propidium iodide (PI), dead cells (PI+) were determined by flow cytometry (performed in triplicate, presented as mean ± SEM, n = 3 independent experiments). b Mature T cells were isolated from the lymph nodes and spleen, labeled with CellTrace Violet (Invitrogen), pretreated for 1 h with or without 50 μM zVAD-fmk, and stimulated with 5 μg/mL αCD3 and 1 μg/mL αCD28. At 48 h, cells were harvested, stained with PI, and analyzed by two-color flow cytometry (n = 3 independent experiments). c Western blot of cFLIP, Bcl-XL, and β-Actin in resting and stimulated mature T cells of indicated genotypes. Representative of three independent experiments. d, e Western blots of p65 phosphorylation, total p65, and β-Actin after stimulation of fibroblasts with d 10 ng/mL TNFα (n = 5 independent experiments) or e 1 μg/mL LPS (n = 3 independent experiments). Numbers under each gel indicate fold changes of signals for each lane

We also performed analysis of TCR-induced responses. The data show an increased PI+ population in Ripk1Ripk3Tradd TKO T cells after 48 h TCR stimulation, than wild type control T cells (Fig. 4b). Cell division barely occurred in Ripk1Ripk3Tradd TKO T cells stimulated through the TCR for 48 h, as compared to wild-type control T cells. A single wild-type allele of TRADD provided improvement, as indicated by an intermediate phenotype in Ripk1Ripk3Tradd T cells, which displayed moderately decreased cell death and moderately increased proliferation, when compared to Ripk1Ripk3Tradd TKO T cells (Fig. 4b). The defect in Ripk1Ripk3Tradd T cells remains significant when compared with wild-type T cells.

To determine the nature of the TCR-induced cell death, T cells were treated with the wide-spectrum caspase inhibitor zVAD-fmk. This treatment rescued Ripk1Ripk3Tradd T cells from death during TCR stimulation (Fig. 4b). Moreover, zVAD treatments greatly improved TCR-induced cell division/proliferation in Ripk1Ripk3Tradd T cells as well as in Ripk1Ripk3Tradd T cells (Fig. 4b). Therefore, these mutant TKO T cells undergo enhanced apoptosis in response to TCR stimulation, despite a resistance to TNFα-induced apoptosis (Fig. 4a). To understand further the enhanced apoptosis phenotype, western blotting analysis was performed to examine anti-apoptotic proteins. Freshly isolated resting Ripk1Ripk3Tradd T cells have lower expression of cFLIP than in Ripk1Ripk3Tradd and WT T cells (Fig. 4c). In contrast, Bcl-XL is barely detectable in freshly isolated resting T cells, and is highly induced at 16 h post stimulation with anti-CD3/CD28 antibodies (Fig. 4c). This TCR-induced Bcl-XL expression is severely impaired in Ripk1Ripk3Tradd T cells, as compared to Ripk1Ripk3Tradd and WT T cells (Fig. 4c). These two proteins are critical for T cell survival by inhibiting apoptosis and are target genes of the pro-survival NFκB pathway. Ripk1Ripk3Tradd T cells do appear to have reduced NFκB signaling, as indicated by reduced phosphorylation of NFκB p65 (Supplementary Fig. 8a). Ripk1Ripk3Tradd T cells also display elevated caspase activation upon stimulation (Supplementary Fig. 8b). Similarly, significantly elevated active caspase 3 levels were present in Ripk1Ripk3Tradd large intestine (Supplementary Fig. 8c). In total, a single wild-type Tradd allele is advantageous for Ripk1Ripk3 T cell survival after stimulation through the TCR. Moreover, the data clearly indicate that TRADD has a dual function in T cells.

A critical role for TRADD in TNFα-mediated NFκB signaling

The ability to activate this pathway by TNFα was analyzed by examining induction of phosphorylation of NFκB p65 (p-p65) in mouse fibroblasts. In WT fibroblasts rapid phosphorylation of p65 was induced within 5 min of TNFα treatment, but NFκB activation is completely blocked in Ripk1Ripk3Tradd fibroblasts (Fig. 4d). A single allele of TRADD in Ripk1Ripk3Tradd fibroblasts results in an intermediate phenotype, that is a delayed activation of the NFκB pathway (Fig. 4d). We treated wild-type and mutant fibroblasts with LPS and analyzed NFκB induction. Ripk1Ripk3Tradd fibroblasts were capable of phosphorylating NFκB, albeit at slightly lower levels than WT fibroblasts (Fig. 4e). In total, TRADD is essential for TNFα-induced NFκB activation in Ripk1Ripk3 fibroblasts, but is dispensable for LPS-induced NFκB activation.

It is possible that the loss of DP thymocytes in Ripk1Ripk3Tradd (Fig. 3a) is secondary to the wasting disease (Fig. 1c). To this end, we analyzed younger mice (3 week old) which have no apparent wasting disease. The lymphoid organs of young Ripk1Ripk3Tradd mice are normal, as compared to WT controls (Supplementary Fig. 9a). There was no obvious defect in the thymus, and mature T cell numbers in lymph nodes was reduced in Ripk1Ripk3Tradd mice (Supplementary Fig. 9b, c). Thymocytes isolated from 3 week old Ripk1Ripk3Tradd mice are also resistant to TNFα-induced cell death unlike Ripk1Ripk3Tradd thymocytes (Supplementary Fig. 10), similar to the result in Fig. 4a. We performed analysis of NFκB target genes in TNFα-treated fibroblasts, and the data indicates impaired NFκB signaling in TKO cells. TNFAIP3 (or A20), an NFκB target gene, is downregulated in Ripk1Ripk3Tradd (TKO), compared to WT ear fibroblasts and is downregulated to a lesser degree in Ripk1Ripk3Tradd cells (Supplementary Fig. 11). We further analyzed in vivo response to TNFα treatment, by intravenous administration in mice. Ripk1Ripk3Tradd mice displayed a rapid drop in body temperature and succumbed to TNFα-induced shock, similar to WT mice (Fig. 5a, b). However, Ripk1Ripk3Tradd mice and Ripk3 mice did not display a significant drop in body temperature and did not succumb to TNFα-induced shock (Fig. 5a, b).

Fig. 5

Ripk1Ripk3Tradd mice succumb to lethal TNFα-induced shock. a Body temperatures of mice of indicated genotypes over time. t tests show a significant difference in body temperature between Ripk1Ripk3Tradd and Ripk1Ripk3Tradd mice at 2 h and 4 h after injection. Graph presented as mean ± SEM. *p < 0.05 as determined by Student’s t test. b Kaplan–Meier plot showing survival of mice of indicated genotypes. Logrank test shows a significant difference in survival between Ripk1Ripk3Tradd and Ripk1Ripk3Tradd mice (p = 0.0069)

Discussion

The TRADD protein was identified almost a quarter century ago, as a potential adaptor molecule for TNFR1 signaling[35]. It was not until the first TRADD KO mice were generated in the year 2008 that the interrogation of the physiological function of TRADD became possible[37-39]. However, Tradd mice show no apparent developmental defect, which is in sharp contrast to the perinatal lethality in Ripk1 mice[30], and to the mid-gestation lethality in Fadd mice[23,24,26]. Furthermore, Tradd mice contain a normal lymphoid compartment, while conditional deletion of RIPK1 or FADD results in T lymphopenia in the periphery[31,42]. These observations appear to indicate TRADD being dispensable during mouse development and lymphocyte development.

Despite having no impact on mouse development, deletion of the necroptosis effector protein kinase RIPK3 completely rescues Fadd mice from embryonic lethality, and the resulting FaddRipk3 DKO mice show progressive lymphoproliferative disease after reaching adulthood[29]. The fact that absence of TRADD is unable to rescue the embryonic defect in Fadd mice (Supplementary Fig. 2) indicates that TRADD is dispensable for necroptotic signaling in vivo. Previous studies have indicated a potential role for TRADD in necroptosis, as indicated by Tradd MEFs being resistant to treatment with TNFα, cycloheximide and zVAD (TCZ)[38]. This observation also contrasts the normal embryogenesis in FaddRipk1 mice[26]. Therefore, necroptosis in embryonic cells is TRADD-independent and occurs directly via RIPK1 in vivo when FADD-mediated apoptosis is inactivated.

Mice lacking the adaptor kinase RIPK1 die shortly after birth, and blocking necroptosis through deletion of the downstream RIPK3, or blocking apoptosis via deletion of FADD provides little survival advantage, because the resulting FaddRipk1 or Ripk1Ripk3 DKO mice die within several days after birth[26,29,32,33]. Only when both RIPK3 and FADD are deleted do Ripk1 mice survive to adulthood[29,32,33]. The striking observation in the current study that deletion of TRADD rescues Ripk1Ripk3 mice from perinatal lethality (Fig. 1) indicates that TRADD, similar to FADD, may mediate apoptosis in neonatal Ripk1Ripk3 mice and cause perinatal death. Therefore, a lack of phenotype in Tradd mice is due in part to the presence of RIPK1.

Although both FADD and TRADD mediate apoptosis, functional distinction between these two adaptors is evident. Unlike Ripk1Ripk3Fadd mice, which have normal postnatal development, Ripk1Ripk3Tradd mice show greatly diminished post-weaning survival (Fig. 1b). The lethality in young adulthood may be explained in part by the severe defect in the intestine in Ripk1Ripk3Tradd mice (Fig. 1e). The elevated Caspase 3 activation is indicative of enhanced apoptosis in the intestine when both alleles of Tradd are inactivated in Ripk1Ripk3 mice (Fig. 1e). Interestingly, these symptoms are reversed by retaining a single wild-type allele of Tradd in Ripk1Ripk3 mice (Fig. 2), contrasting heterozygosity of FADD which is not sufficient to prevent lethality in Ripk1Ripk3 mice during perinatal development[29]. While reducing apoptosis in the intestine, the rescue through haplosufficiency of TRADD did not lead to lymphadenopathy and splenomegaly in the resulting Ripk1Ripk3Tradd mice (Supplementary Fig. 4), unlike the severe lpr-like symptom in Ripk3Fadd or Ripk1Ripk3Fadd mice (Supplementary Fig. 4)[29,33].

In addition to the intestinal defect, Ripk1Ripk3Tradd TKO mice display severe T lymphopenia (Fig. 3). Interestingly, like in wild-type mice, TKO thymocytes and peripheral mature T cells are resistant to cell death triggered by TNFα (Fig. 4a and Supplementary Fig. 7,b). However, presence of one allele of wild-type TRADD rendered Ripk1Ripk3 thymocytes and mature T cells hypersensitive to TNFα-induced death (Fig. 4a and Supplementary Fig. 7b, d). In total, the data argue that TRADD indeed mediates TNFα-induced apoptosis in T cells, when RIPK1 is absent. Therefore, T lymphopenia in the Ripk1Ripk3Tradd mice is counterintuitive, given that thymocytes from these TKO mice are completely resistant to TNFα-induced apoptosis. This paradox can be explained in part by the defect in TCR-induced survival and proliferative responses in peripheral mature Ripk1Ripk3Tradd TKO T cells (Fig. 4b). More importantly, TRADD haplosufficiency, as observed in Ripk1Ripk3Tradd mice, indicates a dual role for TRADD in signaling TCR-induced survival and proliferative responses (Fig. 4b).

The severely impaired proliferative responses to TCR stimulation in Ripk1Ripk3Tradd TKO mice appear to be due largely to caspase-dependent apoptosis, because the wide-spectrum caspase inhibitor zVAD suppressed TCR-induced death in Ripk1Ripk3Tradd TKO T cells and improved cell division/proliferation (Fig. 4b and Supplementary Fig. 8,b). It is important to iterate that death receptor-induced apoptosis is not affected by deletion of TRADD, as the TKO T cells behave similarly to wild-type T cells in response to stimulation of Fas and TNFR1 (Fig. 4a and Supplementary Fig. 7a–c). Therefore, the result in Fig. 4b implies a role for TRADD in TCR-induced survival signaling, beyond its role in promoting TNFR1-induced apoptosis as it does in perinatal cells.

One possible explanation for the improved survival of Ripk1Ripk3Tradd cells and mice is the requirement for TRADD in NFκB signaling. Indeed, we found that dermal fibroblasts of adult TKO mice show a complete blockage of TNFα-mediated NFκB signaling as shown by lack of phosphorylation of p65 (Fig. 4d). This indicates that TRADD is essential for NFκB signaling in the absence of RIPK1. Due to the severe defects in the intestines and T cells of TKO mice, there may be a cell type-specific requirement for TRADD-mediated NFκB signaling to prevent apoptosis. Further supporting this notion is the observation that TKO T cells have lower expression of the NFκB targets, cFLIP and Bcl-XL, as compared with Ripk1Ripk3Tradd or wild-type cells (Fig. 4c). In total, similar to RIPK1 and FADD, TRADD can also initiate or protect against cell death depending on the conditions and cell type.

In summary, the findings in the current study identify a previously unappreciated role for TRADD during perinatal development and in adult mice lacking RIPK1 and RIPK3. Gene dosage of TRADD elicits a Goldilocks effect on the survival of Ripk1Ripk3 mice, whereby Tradd and Tradd results in death by apoptosis while haplosufficiency of TRADD proves to be optimal for survival. These findings demonstrate the interplay between cell death and NFκB signaling, identify TRADD as a key component in cell death regulation, and provide insight into the workings of the apoptotic pathway in the presence and absence of RIPK1.

Methods

Mice

Both males and females of mus musculus in a C57BL/6 background were used. Tradd mice were provided by Liu[38]. Ripk1 mice were provided by Kelliher[30]. Ripk3 mice were provided by Newton and Dixit[43]. All mice were bred and housed in a specific pathogen free environment at Thomas Jefferson University. After intercrosses of indicated mice, neonates were monitored daily for survival from birth to weaning age and deceased pups were immediately collected and genotyped. Viable pups were genotyped at weaning age and monitored for survival. Kaplan–Meier survival curves were generated using Prism software (Graphpad Software, Inc.). All animal studies are approved by the Institutional Animal Care and Use Committee (IACUC) at Thomas Jefferson University. For genotyping, DNA was isolated from the ear of mice with an alkaline lysis solution containing 25 mM NaOH and 0.2 mM EDTA followed by neutralization with 40 mM Tris-HCl pH 5.0. Allele specific primers were used to amplify wild type and knockout alleles as previously described for Fadd[23] (WT primers: 5′- ATG GAC CCA TTC CTG GTG CTG CTG-3′; 5′-CAG TAG ATC GTG TCG GCG CAG CG-3′ and KO primers: 5′-ACTGTAGTGCCCAGCAGAGACCAGC -3′; 5′-CGCTCGGTGTTCGAGGCCACACGC-3′); Ripk1[30] (WT&KO primers: 5′-CTGCTAAAGCGCATGCTC-3′; 5′-TGTGTCAAGTCTCCCTGCAG-3′; 5′-CACGGTCCTTTTGCCCTG-3′); Tradd[38] (WT primers: 5′-CACTGACTCTTCAAGACCAGCAGAC-3′; 5′-CAACAGATCCTCTGCTAGACTAGTG-3′ and KO primers: 5′-TGCACATGTGTCCTCGAGTG-3′; 5′-GGAGAGCTTGGCTGTCTTGG-3′); Ripk3[43] (WT&KO primers: 5′-CGCTTTAGAAGCCTTCAGGTTGAC-3′; 5′-GCCTGCCCATCAGCAACTC-3′; 5′-CCAGAGGCCACTTGTGTAGCG-3′).

Embryo analysis

Mating of FaddTradd mice was setup in the evening and females were checked in the morning. When vaginal plug was detected, embryos were designated E0.5. Embryos were isolated from pregnant mice at E12.5-E18.5 and genotyped as described above.

Cell culture including fibroblast and T cell preparation

Thymocytes and peripheral T cells purified from spleen and lymph nodes were cultured in complete RPMI media (cRPMI) containing 10% FBS, 2 mM L-Glutamine, penicillin (100 U/mL), streptomycin (100 μg/mL), and β-mercaptoethanol (50 μM). Fibroblasts were isolated from the ears of euthanized adult mice was adapted from previously established protocol[44]. In summary, ears were sterilized with 70% ethanol and cut into small pieces. Ear pieces were incubated with warm 0.25% trypsin at 37 °C, 5% CO2 for 90 min, then broken up and passed through a cell strainer with complete DMEM (cDMEM) containing 10% FBS, 1mM L-Glutamine, penicillin (100 U/mL), streptomycin (100 μg/mL), and β-mercaptoethanol (50 μM). Isolated cells were washed once in cDMEM and cultured on tissue culture-treated plates at 37 °C, 5% CO2.

Histology

Intestines were prepared for histology using the swiss roll technique followed by fixation in 10% formalin for 24 h. Samples were washed in PBS, placed in 70% ethanol, and then embedded in formalin. Sections were stained with hematoxylin and eosin or with anti-cleaved caspase 3 antibody (Cell Signaling Technology #9661).

Flow cytometry

Spleen, lymph nodes, and thymus were isolated during mouse dissection. Bone marrow was isolated from tibias and femurs of mice. Single cell suspension was prepared and red blood cells were depleted by hypotonic lysis. Cells were stained on ice for 30 minutes in PBS containing 3% BSA, 1 mM EDTA, and 0.05% sodium azide with fluorochrome-conjugated antibodies anti-CD3 (BD Biosciences, clone 17A2), anti-CD4 (BD Biosciences, clone GK1.5), anti-CD8 (Caltag, clone CT-CD8a), anti-B220 (Caltag, clone RA3-6B2), anti-IgM (Jackson ImmunoResearch, 115-116-075), anti-Mac1 (BD Biosciences, clone M1/70), anti-Gr1 (eBioscience, clone RB6-8C5). Samples were washed twice with PBS. Data was acquired on LSR II flow cytometric analyzer (BD Biosciences) and analyzed using FlowJo software (Treestar). Total cell numbers were counted on Countess Automated Cell Counter (Invitrogen) and lymphocyte cellularity was determined by multiplying the total cell number of indicated organ by percentage of CD3+ or B220+ cells within the organ obtained by flow cytometry.

Cell death assays

Thymocytes (105) or purified mature peripheral T cells were seeded in triplicate in 96 well flat-bottom plates in cRPMI with various concentrations of anti-Fas antibody (BD Biosciences, clone Jo2), mouse TNFα (Alexis Pharmaceuticals), or human TNFα (Life Technologies) for 16 h with cycloheximide. In indicated experiments, thymocytes were pre-treated with TNFR1 or TNFR2 blocking antibodies (Life Technologies) in complete RPMI on ice for 4 h before TNFα treatment. Mature peripheral T cells were treated with FLAG-tagged sFasL (Alexis Pharmaceuticals) and anti-FLAG M2 antibody (1 μg/mL, Sigma). After incubation, 1 μg/mL propidium iodide (PI) and the percentage of cell death was analyzed by flow cytometry as determined by PI+ cells.

T cell proliferation assays

Mature T cells were purified of the spleen and lymph nodes by staining with anti-Thy1.2 antibody (BD Biosciences, clone 53–2.1) and sorting Thy1.2+ cells using a FACS Aria (BD Biosciences). For fluorescent labeling, purified T cells (106/mL) were labeled with 1 μM CellTraceTM Violet in PBS for 20 min at 37 °C, 5% CO2. cRPMI (5 mL) was added to each tube and incubated for an additional 5 min at 37 °C 5% CO2. Labeled cells were then centrifuged for 5 minutes, supernatant was removed, and cells were resuspended in cRPMI. Labeled T cells (2 × 105) were seeded in 96 well round bottom plates pre-coated with 5 μg/mL anti-CD3 (BD Biosciences, clone 145-2C11) in cRPMI with 1 μg/mL anti-CD28 antibodies (BD Biosciences, clone 37.51). At indicated time points, T cells were collected, 1 μg/mL PI was added, and cells were analyzed by two-color flow cytometry on the LSR II (BD Biosciences) for cell death and division kinetics. Data was analyzed using FlowJo software (Treestar). Caspase activities in purified mature T cells was measured using CellEventTM Caspase 3/7 Green Flow Cytometry Assay Kit (Life Technology), per the manufacturer’s instructions. Briefly, T cells were stimulated with 5 μg/mL anti-CD3 and 1 μg/mL anti-CD28 antibodies for the given time points. CellEvent Caspase 3/7 Green Detection Reagent was added to T cell culture, followed by 30 min incubation at 37 °C, 5% CO2. Samples were immediately run on LSR II cytometer (BD Biosciences) and data analyzed using FlowJo Software (Treestar).

Western blotting analysis

To prepare samples for western blot, single cell suspension of splenocytes was prepared from mice of indicated genotypes to detect the presence or absence of RIPK1, RIPK3, and TRADD. T cells were enriched from the spleen and lymph nodes of mice of indicated genotypes by negative selection using MojoSort Mouse CD3 T cell Isolation kit (Biolegend, #480031) and stimulated with 5 μg/mL anti-CD3 and 1 μg/mL anti-CD28 antibodies in cRPMI for 16 h or with 1x eBioscience Cell Stimulation Cocktail of PMA/Ionomycin (Invitrogen) for 5 min. Fibroblasts were stimulated with 10 ng/mL TNFα or 1 μg/mL LPS for indicated time points. Cell lysates were prepared in cold RIPA lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Nonidet P-40, 0.5% deoxycholate, 0.1% SDS, 1 mM phenylmethyl sulphonyl fluoride (PMSF), and 1X Halt protease inhibitor cocktail (Thermo Scientific). Additional inhibitors were added when probing for phospho-specific proteins (50 mM β-glycerophosphate, 1 mM sodium vanadate, 10 mM sodium fluoride). Proteins (40 μg) were separated on a 10% SDS-PAGE gel and transferred to a nitrocellulose membrane. Membranes were blocked with 5% milk in TBST and blotted with antibodies specific for RIPK1 (BD Biosciences, #610459), RIPK3 (ProSci, #2283), TRADD (Santa Cruz, sc7868), cFLIP (Alexis Biochemicals, ALX-804-127), Bcl-XL (Cell Signaling #2764), total p65 (Cell Signaling #3034), or β-Actin (Sigma Aldrich, A-5441) in 5% milk in TBST at 4 °C overnight. For phospho-specific antibodies, membranes were blocked with 5% BSA in TBST for 1 h at room temperature and blotted overnight with anti-phosphorylated p65 (Cell Signaling Technology, #3033) in 5% BSA in TBST. Membranes were stained with HRP-conjugated secondary antibodies at room temperature for 1 h. Membranes were treated with Western Lightning Plus-ECL (Perkin Elmer, NEL105001EA) and developed using X-Ray film or FluorChem M Western Blot Imaging machine (ProteinSimple). Quantification of signals on X-ray films was performed using the ImageJ software. Uncropped gels are provided in the Source Data file.

TNFα-induced shock

Ten micrograms of TNFα (Sino Biological) was injected intravenously into mice. Mice were monitored for survival and core body temperature every 2 h for 10 h and at 24 h. Core body temperature was taken with a MicroTherma 2T Hand Held thermometer with rectal probe (Braintree Scientific). Mice were euthanized if moribund or body temperature dropped below 23.6 °C.

Statistical analysis

Data is represented as mean ± standard error of the mean (SEM). Student’s t-tests were performed using Prism software (Graphpad Software, Inc.) to determine p value. A p value < 0.05 was considered significant. Logrank tests were performed to compare survival of various genotypes in Kaplan Meier plots. Chi square analysis was performed to determine significance of genotype tables.