Hossein Shateri1, Akram Ranjbar2, Nejat Kheiripour3, Hassan Ghasemi4, Yasin Pourfarjam5, Elahe Habibitabar1, Hamid Gholami1, Heresh Moridi6. 1. Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. 2. Department of Toxicology and Pharmacology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran. 3. Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan. Iran. 4. Department of Clinical Biochemistry, Abadan School of Medical Sciences, Abadan, Iran. 5. Department of Chemistry, University of Cincinnati, Cincinnati, OH, United States of America. 6. Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address: hereshmoridi@yahoo.com.
Abstract
AIMS: Oxidative stress induced by diabetes mellitus (DM) is considered as one of the main causes of infertility in diabetic patients. The aim of the present study was to assess the effect of Tempol - as a synthetic antioxidant- on the testis oxidative stress and sperm parameters in type 2 diabetic (T2D) rats. MAIN METHODS: Twenty male Wistar rats were divided into 4 groups. Control groups (C) and diabetic groups (D); the control and diabetic groups received Tempol (100 mg/kg) for one month. Sperm parameters and oxidative stress biomarkers were evaluated in testicular tissue. KEY FINDINGS: The results demonstrated that administration of Tempol in diabetic rats improved sperm motility and viability and decreased the count of abnormal sperms. Also Tempol decreased the fasting blood sugar (FBS) and lipid peroxidation (LPO). In addition, Tempol significantly increased total antioxidant capacity (TAC) levels in testis tissue of T2D rats. Histopathological changes were also improved in the diabetic treated group. SIGNIFICANCE: Taken together, the results indicated that Tempol improved fertility parameters in a diabetic rat through reducing oxidative stress.
AIMS: Oxidative stress induced by diabetes mellitus (DM) is considered as one of the main causes of infertility in diabeticpatients. The aim of the present study was to assess the effect of Tempol - as a synthetic antioxidant- on the testis oxidative stress and sperm parameters in type 2 diabetic (T2D) rats. MAIN METHODS: Twenty male Wistar rats were divided into 4 groups. Control groups (C) and diabetic groups (D); the control and diabetic groups received Tempol (100 mg/kg) for one month. Sperm parameters and oxidative stress biomarkers were evaluated in testicular tissue. KEY FINDINGS: The results demonstrated that administration of Tempol in diabeticrats improved sperm motility and viability and decreased the count of abnormal sperms. Also Tempol decreased the fasting blood sugar (FBS) and lipid peroxidation (LPO). In addition, Tempol significantly increased total antioxidant capacity (TAC) levels in testis tissue of T2D rats. Histopathological changes were also improved in the diabetic treated group. SIGNIFICANCE: Taken together, the results indicated that Tempol improved fertility parameters in a diabeticrat through reducing oxidative stress.