Zhe Zheng1,2, Ruijuan Hao1,2, Xinwei Xiong1,2, Yu Jiao1,2, Yuewen Deng3,4, Xiaodong Du5,6. 1. Fishery College, Guangdong Ocean University, Zhanjiang, 524088, China. 2. Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province, Zhanjiang, 524088, China. 3. Fishery College, Guangdong Ocean University, Zhanjiang, 524088, China. dengyw@gdou.edu.cn. 4. Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province, Zhanjiang, 524088, China. dengyw@gdou.edu.cn. 5. Fishery College, Guangdong Ocean University, Zhanjiang, 524088, China. zjduxd@126.com. 6. Pearl Breeding and Processing Engineering Technology Research Center of Guangdong Province, Zhanjiang, 524088, China. zjduxd@126.com.
Abstract
BACKGROUND: Marine bivalves undergo complex development processes, such as shell morphology conversion and changes of anatomy and life habits. In this study, the transcriptomes of pearl oyster Pinctada fucata martensii and Pacific oyster Crassostrea gigas at different development stages were analyzed to determine the key molecular events related to shell formation, settlement and metamorphosis. RESULT: According to the shell matrix proteome, biomineralization-related genes exhibited a consensus expression model with the critical stages of shell formation. Differential expression analysis of P. f. martensii, revealed the negative regulation and feedback of extracellular matrixs as well as growth factor pathways involved in shell formation of larvae, similar to that in C. gigas. Furthermore, neuroendocrine pathways in hormone receptors, neurotransmitters and neuropeptide receptors were involved in shell formation, settlement and metamorphosis. CONCLUSION: Our research demonstrated the main clusters of regulation elements related to shell formation, settlement and metamorphosis. The regulation of shell formation and metamorphosis could be coupled forming the neuroendocrine-biomineralization crosstalk in metamorphosis. These findings could provide new insights into the regulation in bivalve development.
BACKGROUND: Marine bivalves undergo complex development processes, such as shell morphology conversion and changes of anatomy and life habits. In this study, the transcriptomes of pearl oyster Pinctada fucata martensii and Pacific oysterCrassostrea gigas at different development stages were analyzed to determine the key molecular events related to shell formation, settlement and metamorphosis. RESULT: According to the shell matrix proteome, biomineralization-related genes exhibited a consensus expression model with the critical stages of shell formation. Differential expression analysis of P. f. martensii, revealed the negative regulation and feedback of extracellular matrixs as well as growth factor pathways involved in shell formation of larvae, similar to that in C. gigas. Furthermore, neuroendocrine pathways in hormone receptors, neurotransmitters and neuropeptide receptors were involved in shell formation, settlement and metamorphosis. CONCLUSION: Our research demonstrated the main clusters of regulation elements related to shell formation, settlement and metamorphosis. The regulation of shell formation and metamorphosis could be coupled forming the neuroendocrine-biomineralization crosstalk in metamorphosis. These findings could provide new insights into the regulation in bivalve development.
Authors: Shannon H Cole; Ginger E Carney; Colleen A McClung; Stacey S Willard; Barbara J Taylor; Jay Hirsh Journal: J Biol Chem Date: 2005-02-03 Impact factor: 5.157
Authors: Maria Byrne; Demian Koop; Dario Strbenac; Paula Cisternas; Regina Balogh; Jean Yee Hwa Yang; Phillip L Davidson; Gregory Wray Journal: DNA Res Date: 2020-02-01 Impact factor: 4.458