| Literature DB >> 30735542 |
Yanyu Wang, Sarah A Jenkins, Chunfang Gu, Ankita Shree, Margarita Martinez-Moczygemba, Jennifer Herold, Marina Botto, Rick A Wetsel, Yi Xu.
Abstract
[This corrects the article DOI: 10.1371/journal.ppat.1005678.].Entities:
Year: 2019 PMID: 30735542 PMCID: PMC6368309 DOI: 10.1371/journal.ppat.1007579
Source DB: PubMed Journal: PLoS Pathog ISSN: 1553-7366 Impact factor: 6.823
Fig 1B. anthracis spore surface protein BclA mediated CFH binding to spores.
Spores were incubated with purified human CFH in PBS buffer containing D-alanine. Spore-bound CFH was determined by flow cytometry (A), solid phase binding assay (B) and Western blot (C). Flow cytometry results were combined from at least three independent experiments. Solid phase binding assay results were combined from two independent experiments, each with duplicate wells. Western blots shown were representative of at least three independently performed experiments. (D) Recombinant BclA protein (rBclA) bound to immobilized human CFH in a concentration-dependent manner. Results were combined from three independent experiments. *, p < 0.05; **, p < 0.01; ***, p < 0.001; t test.