Olga Kukuy1, Batia Kaplan2, Sizilia Golderman3, Alexander Volkov4, Adrian Duek5, Merav Leiba6,7, Ilan Ben-Zvi3,7,8, Avi Livneh3,7,8. 1. Dialysis Unit, Institute of Nephrology and Hypertension, Sheba Medical Center, Tel Hashomer, Ramat Gan, Israel. 2. Laboratory of FMF and Amyloidosis, Heller Institute of Medical Research, Sheba Medical Center, Tel Hashomer, 52621, Ramat Gan, Israel. batiakaplan@gmail.com. 3. Laboratory of FMF and Amyloidosis, Heller Institute of Medical Research, Sheba Medical Center, Tel Hashomer, 52621, Ramat Gan, Israel. 4. Institute of Pathology, Sheba Medical Center, Tel Hashomer, Ramat Gan, Israel. 5. Hematology Institute, Sheba Medical Center, Tel Hashomer, Ramat Gan, Israel. 6. Hematology Department, Assuta Medical Center, Ashdod, Israel. 7. Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel. 8. Department of Internal Medicine F, Sheba Medical Center, Tel Hashomer, Ramat Gan, Israel.
Abstract
BACKGROUND: Plasma cell dyscrasias (PCD) comprise a wide spectrum of disorders, which may adversely affect the kidney. However, in some PCD cases associated with kidney disease, the routine laboratory tests may be incapable to determine precisely the form of PCD, i.e., benign or malignant. Moreover, the kidney biopsy needed for precise diagnosis may be risky or declined. To overcome these limitations, we have developed and reported a new non-invasive technique based on serum free light chains (FLC) monomer (M) and dimer (D) pattern analysis (FLC MDPA), which allowed differentiation between malignant and benign PCD forms. The objective of our retrospective study was to demonstrate the utility of FLC MDPA in solving ten puzzling PCD cases complicated with kidney disease (patients 1-10). METHODS: Ten patients with uncertain form of PCD or with a questionable response to treatment were studied. In addition to routine laboratory tests and clinical evaluation of the PCD patients, our previously developed FLC MDPA in sera and biochemical amyloid typing in biopsy tissues were applied. RESULTS: The FLC MDPA aided the diagnosis of the PCD underlying or accompanying the kidney disease in patients 1-5, and helped to interpret properly the response to treatment in patients 1, 6-10. The FLC MDPA findings were confirmed by a biochemical analysis of tissue amyloid deposits and subsequently by the outcome of these patients. CONCLUSIONS: FLC MDPA is a non-invasive diagnostic test useful in the management of ambiguous cases of PCD associated with kidney disease.
BACKGROUND:Plasma cell dyscrasias (PCD) comprise a wide spectrum of disorders, which may adversely affect the kidney. However, in some PCD cases associated with kidney disease, the routine laboratory tests may be incapable to determine precisely the form of PCD, i.e., benign or malignant. Moreover, the kidney biopsy needed for precise diagnosis may be risky or declined. To overcome these limitations, we have developed and reported a new non-invasive technique based on serum free light chains (FLC) monomer (M) and dimer (D) pattern analysis (FLC MDPA), which allowed differentiation between malignant and benign PCD forms. The objective of our retrospective study was to demonstrate the utility of FLC MDPA in solving ten puzzling PCD cases complicated with kidney disease (patients 1-10). METHODS: Ten patients with uncertain form of PCD or with a questionable response to treatment were studied. In addition to routine laboratory tests and clinical evaluation of the PCDpatients, our previously developed FLC MDPA in sera and biochemical amyloid typing in biopsy tissues were applied. RESULTS: The FLC MDPA aided the diagnosis of the PCD underlying or accompanying the kidney disease in patients 1-5, and helped to interpret properly the response to treatment in patients 1, 6-10. The FLC MDPA findings were confirmed by a biochemical analysis of tissue amyloid deposits and subsequently by the outcome of these patients. CONCLUSIONS:FLC MDPA is a non-invasive diagnostic test useful in the management of ambiguous cases of PCD associated with kidney disease.
Authors: Lawreen H Connors; Yan Jiang; Marianna Budnik; Roger Théberge; Tatiana Prokaeva; Kip L Bodi; David C Seldin; Catherine E Costello; Martha Skinner Journal: Biochemistry Date: 2007-11-16 Impact factor: 3.162
Authors: Helen J Lachmann; Ruth Gallimore; Julian D Gillmore; Hugh D Carr-Smith; Arthur R Bradwell; Mark B Pepys; Philip N Hawkins Journal: Br J Haematol Date: 2003-07 Impact factor: 6.998