| Literature DB >> 30732819 |
Wen-Na Zhang1, Ri-Na Su2, Li-Li Gong2, Wei-Wei Yang2, Juan Chen2, Rui Yang2, Ya Wang2, Wen-Juan Pan1, Yong-Ming Lu3, Yan Chen4.
Abstract
In this research, a polysaccharide fraction (EFSP-1) was obtained from the seeds of Euryale ferox Salisb. by DEAE sepharose FF and Superdex™ 75 gel chromatography. The average molecular weight (Mw) of EFSP-1 was 8.75 kDa. Monosaccharides composition analysis indicated that EFSP-1 was a glucan. The structure of EFSP-1 was characterized by analysis of FT-IR, GC-MS and NMR, which indicated that the backbone of EFSP-1 was mainly composed of (1→4)-α-D-Glcp with branches substituted at O-6 and terminated with T-α-D-Glcp. Moreover, the hypoglycemic effect of EFSP-1 was investigated by establishing insulin resistance HepG2 and 3T3-L1 cells. The results showed that EFSP-1 could increase glucose consumption by up-regulating the expression of GLUT-4 via activating PI3K/Akt signal pathway in IR cells. Hence, EFSP-1 could be a potential functional food to ameliorate insulin resistance for diabetes therapy.Entities:
Keywords: 1-phenyl-3-methyl-5-pyrazolone (PubChem CID: 90474051); 3T3-L1 cells; Acetic anhydride (PubChem CID: 7918); D-arabinose (PubChem CID: 229); D-galactose (PubChem CID: 6036); D-glucose (PubChem CID: 5793); D-mannose (PubChem CID: 18950); Dimethyl sulfoxide (PubChem CID: 679); Euryale feroxseed; Glucan; Glucuronic acid (PubChem CID: 94715); HepG2 cells; Hypoglycemic activity; Insulin resistance; Methyl iodide (PubChem CID: 6328); Pyridine (PubChem CID: 1049); Sodium borohydride (PubChem CID: 4311764); Sodium hydroxide (PubChem CID: 14798); Trifluoroacetic acid (PubChem CID: 6422)
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Year: 2019 PMID： 30732819 DOI： 10.1016/j.carbpol.2019.01.044
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381