Literature DB >> 30729461

2D and 3D In Vitro Co-Culture for Cancer and Bone Cell Interaction Studies.

Silvia Marino1, Ryan T Bishop2, Daniëlle de Ridder2, Jesus Delgado-Calle3, Michaela R Reagan4.   

Abstract

Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.

Entities:  

Keywords:  Cancer cells; In vitro co-culture; Osteoblasts; Osteoclasts; Osteocytes

Mesh：

Year:  2019        PMID： 30729461     DOI： 10.1007/978-1-4939-8997-3_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

Review 1.  In Vitro 3D Cultures to Reproduce the Bone Marrow Niche.

Authors:  Justin Ham; Lauren Lever; Maura Fox; Michaela R Reagan
Journal:  JBMR Plus       Date:  2019-10-01

Review 2.  Critical Assessment of In Vitro and In Vivo Models to Study Marrow Adipose Tissue.

Authors:  Michaela R Reagan
Journal:  Curr Osteoporos Rep       Date:  2020-04       Impact factor: 5.096

Review 3.  The Role of Notch and Wnt Signaling in MSC Communication in Normal and Leukemic Bone Marrow Niche.

Authors:  Paul Takam Kamga; Riccardo Bazzoni; Giada Dal Collo; Adriana Cassaro; Ilaria Tanasi; Anna Russignan; Cristina Tecchio; Mauro Krampera
Journal:  Front Cell Dev Biol       Date:  2021-01-08

4.  GFI1-Dependent Repression of SGPP1 Increases Multiple Myeloma Cell Survival.

Authors:  Daniela N Petrusca; Patrick L Mulcrone; David A Macar; Ryan T Bishop; Evgeny Berdyshev; Attaya Suvannasankha; Judith L Anderson; Quanhong Sun; Philip E Auron; Deborah L Galson; G David Roodman
Journal:  Cancers (Basel)       Date:  2022-02-02       Impact factor: 6.639

5.  In 2D and 3D Cell Culture Models, Effects of Endothelial Cells on E-cadherin / β-catenin Expression Levels and Spheroid Sizes in Ishikawa Cells.

Authors:  Mervenur Kalender; Muhammet Volkan Bulbul; Bircan Kolbasi; Ilknur Keskin
Journal:  Asian Pac J Cancer Prev       Date:  2022-01-01

6.  3D cell culture stimulates the secretion of in vivo like extracellular vesicles.

Authors:  Sirisha Thippabhotla; Cuncong Zhong; Mei He
Journal:  Sci Rep       Date:  2019-09-10       Impact factor: 4.379

7.  JZL184, A Monoacylglycerol Lipase Inhibitor, Induces Bone Loss in a Multiple Myeloma Model of Immunocompetent Mice.

Authors:  Silvia Marino; Giovana Carrasco; Boya Li; Karan M Shah; Darren L Lath; Antonia Sophocleous; Michelle A Lawson; Aymen I Idris
Journal:  Calcif Tissue Int       Date:  2020-04-13       Impact factor: 4.333
  7 in total

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