Literature DB >> 30729460

Isolation, Differentiation, and Characterization of Human Bone Marrow Stem Cells In Vitro and In Vivo.

Janos Kanczler1, Rahul S Tare1, Patrick Stumpf1, Timothy J Noble1, Cameron Black1, Richard O C Oreffo2.   

Abstract

In this chapter, we describe techniques for the isolation and characterisation of skeletal stem cells from human bone marrow. The methods for enrichment of STRO-1+ and STRO-4+ cells using magnetic activated cell sorting are described and we also detail techniques for establishing and characterizing osteogenic, adipogenic, and chondrogenic cultures from these cells. Finally, we present methods for studying the ability of these cells to produce bone in vivo using diffusion chambers which have been implanted subcutaneously into mice.

Entities:  

Keywords:  Adipocyte; Bone marrow; Chondrocyte; Diffusion chamber; Osteoblast; Stem cells

Mesh：

Substances：

Year:  2019        PMID： 30729460     DOI： 10.1007/978-1-4939-8997-3_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

Review 1.  Modifying MSC Phenotype to Facilitate Bone Healing: Biological Approaches.

Authors:  Stuart B Goodman; Tzuhua Lin
Journal:  Front Bioeng Biotechnol       Date:  2020-06-24

2.  Cell-controlled dynamic surfaces for skeletal stem cell growth and differentiation.

Authors:  Hilary J Anderson; Jugal Kishore Sahoo; Julia Wells; Sebastiaan van Nuffel; Hala S Dhowre; Richard O C Oreffo; Mischa Zelzer; Rein V Ulijn; Matthew J Dalby
Journal:  Sci Rep       Date:  2022-05-17       Impact factor: 4.996

3.  Genetically-programmed, mesenchymal stromal cell-laden & mechanically strong 3D bioprinted scaffolds for bone repair.

Authors:  Hosam Al-Deen M Abu Awwad; Lalitha Thiagarajan; Janos M Kanczler; Mahetab H Amer; Gordon Bruce; Stuart Lanham; Robin M H Rumney; Richard O C Oreffo; James E Dixon
Journal:  J Control Release       Date:  2020-07-03       Impact factor: 9.776

4.  Hurdles to uptake of mesenchymal stem cells and their progenitors in therapeutic products.

Authors:  Peter G Childs; Stuart Reid; Manuel Salmeron-Sanchez; Matthew J Dalby
Journal:  Biochem J       Date:  2020-09-18       Impact factor: 3.857

5.  The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells.

Authors:  Tom Hodgkinson; P Monica Tsimbouri; Virginia Llopis-Hernandez; Paul Campsie; David Scurr; Peter G Childs; David Phillips; Sam Donnelly; Julia A Wells; Fergal J O'Brien; Manuel Salmeron-Sanchez; Karl Burgess; Morgan Alexander; Massimo Vassalli; Richard O C Oreffo; Stuart Reid; David J France; Matthew J Dalby
Journal:  Sci Adv       Date:  2021-02-26       Impact factor: 14.136

6.  Transfer learning efficiently maps bone marrow cell types from mouse to human using single-cell RNA sequencing.

Authors:  Patrick S Stumpf; Xin Du; Haruka Imanishi; Yuya Kunisaki; Yuichiro Semba; Timothy Noble; Rosanna C G Smith; Matthew Rose-Zerili; Jonathan J West; Richard O C Oreffo; Katayoun Farrahi; Mahesan Niranjan; Koichi Akashi; Fumio Arai; Ben D MacArthur
Journal:  Commun Biol       Date:  2020-12-04

7.  Nanovibrational Stimulation of Mesenchymal Stem Cells Induces Therapeutic Reactive Oxygen Species and Inflammation for Three-Dimensional Bone Tissue Engineering.

Authors:  Wich Orapiriyakul; Monica P Tsimbouri; Peter Childs; Paul Campsie; Julia Wells; Marc A Fernandez-Yague; Karl Burgess; K Elizabeth Tanner; Manlio Tassieri; Dominic Meek; Massimo Vassalli; Manus J P Biggs; Manuel Salmeron-Sanchez; Richard O C Oreffo; Stuart Reid; Matthew J Dalby
Journal:  ACS Nano       Date:  2020-07-23       Impact factor: 15.881
  7 in total

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