Literature DB >> 30728639

Genome Engineering Approaches to Improve Nosokomycin A Production by Streptomyces ghanaensis B38.3.

Yuriy Kuzhyk1, Maria Lopatniuk2, Andriy Luzhetskyy2, Victor Fedorenko1, Bohdan Ostash1.   

Abstract

Here we describe our efforts to improve the levels of phosphoglycolipid antibiotic nosokomycin A production by Streptomyces ghanaensis ATCC14672 via genome engineering approaches. Introduction of two extra copies of leucyl tRNA (UUA) gene bldA and one copy of moenomycin biosynthesis gene cluster led, on average, to threefold increase in nosokomycin A titers (up to 1.5 mg/L). Our results validate genome engineering approach as a viable strategy to improve moenomycin production.

Entities:  

Keywords:  Genome engineering; Integrative vectors; Moenomycins; Streptomyces ghanaensis

Year:  2018        PMID： 30728639      PMCID： PMC6328414          DOI： 10.1007/s12088-018-0761-x

Source DB:  PubMed          Journal:  Indian J Microbiol        ISSN： 0046-8991            Impact factor:   2.461


  9 in total

1.  Testing the utility of site-specific recombinases for manipulations of genome of moenomycin producer Streptomyces ghanaensis ATCC14672.

Authors:  M Lopatniuk; B Ostash; R Makitrynskyy; S Walker; A Luzhetskyy; V Fedorenko
Journal:  J Appl Genet       Date:  2015-03-24       Impact factor: 3.240

Review 2.  Gene editing for cell engineering: trends and applications.

Authors:  Sanjeev K Gupta; Pratyoosh Shukla
Journal:  Crit Rev Biotechnol       Date:  2016-08-18       Impact factor: 8.429

Review 3.  Moenomycin family antibiotics: chemical synthesis, biosynthesis, and biological activity.

Authors:  Bohdan Ostash; Suzanne Walker
Journal:  Nat Prod Rep       Date:  2010-08-23       Impact factor: 13.423

Review 4.  The Importance of Being Modified: The Role of RNA Modifications in Translational Fidelity.

Authors:  Paul F Agris; Amithi Narendran; Kathryn Sarachan; Ville Y P Väre; Emily Eruysal
Journal:  Enzymes       Date:  2017-04-22

5.  Gene cloning system for sulfonamide-mineralizing Microbacterium sp. strain BR1.

Authors:  I Ostash; B Kolvenbach; P F-X Corvini; V Fedorenko; B Ostash; Danuta Cichocka
Journal:  J Appl Genet       Date:  2018-01-25       Impact factor: 3.240

6.  MoeH5: a natural glycorandomizer from the moenomycin biosynthetic pathway.

Authors:  Bohdan Ostash; Jennifer Campbell; Andriy Luzhetskyy; Suzanne Walker
Journal:  Mol Microbiol       Date:  2013-11-21       Impact factor: 3.501

7.  Identification and characterization of Streptomyces ghanaensis ATCC14672 integration sites for three actinophage-based plasmids.

Authors:  Bohdan Ostash; Roman Makitrinskyy; Suzanne Walker; Victor Fedorenko
Journal:  Plasmid       Date:  2009-01-22       Impact factor: 3.466

8.  Pleiotropic regulatory genes bldA, adpA and absB are implicated in production of phosphoglycolipid antibiotic moenomycin.

Authors:  Roman Makitrynskyy; Bohdan Ostash; Olga Tsypik; Yuriy Rebets; Emma Doud; Timothy Meredith; Andriy Luzhetskyy; Andreas Bechthold; Suzanne Walker; Victor Fedorenko
Journal:  Open Biol       Date:  2013-10-23       Impact factor: 6.411

Review 9.  Glycosyltransferases and Transpeptidases/Penicillin-Binding Proteins: Valuable Targets for New Antibacterials.

Authors:  Eric Sauvage; Mohammed Terrak
Journal:  Antibiotics (Basel)       Date:  2016-02-17
  9 in total
  1 in total

1.  Genetic Engineering of Streptomyces ghanaensis ATCC14672 for Improved Production of Moenomycins.

Authors:  Roman Makitrynskyy; Olga Tsypik; Andreas Bechthold
Journal:  Microorganisms       Date:  2021-12-24
  1 in total

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