Literature DB >> 30727607

RAPD analysis of somaclonal variants derived from embryo callus cultures of peach.

G Hashmi1,2, R Huettel3, R Meyer3, L Krusberg2, F Hammerschlag4.   

Abstract

Peach [Prunus persica (L.) Batsch] regenerants from cv 'Sunhigh' embryo no. 156, regenerants obtained from cv 'Redhaven' embryo no. 30, and two peach cultivars 'Sunhigh' and 'Redhaven', were screened for polymorphic RAPD (Random Amplified Polymorphic DNA) markers with up to 60 10-mer primers. Although 35 primers produced results with scoreable bands, only 10 of the primers revealed polymorphism for regenerants of embryo no. 156 and cv 'Sunhigh', and 1 revealed a low level of polymorphism for regenerants of embryo no. 30 and cv 'Redhaven'. This study demonstrates the feasibility of using RAPD markers to identify somaclonal variants of peach and provides evidence for the existence of genetic differences among these variants.

Entities:  

Keywords:  Prunus persica; Somaclonal variation

Year:  1997        PMID： 30727607     DOI： 10.1007/BF01275503

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  17 in total

1.  Molecular characterisation of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA (RAPD) markers.

Authors:  E Baird; S Cooper-Bland; R Waugh; M DeMaine; W Powell
Journal:  Mol Gen Genet       Date:  1992-06

2.  The detection of somaclonal variants of beet using RAPD.

Authors:  M T Munthali; H J Newbury; B V Ford-Lloyd
Journal:  Plant Cell Rep       Date:  1996-03       Impact factor: 4.570

3.  Detection of DNA sequence polymorphisms among wheat varieties.

Authors:  S He; H Ohm; S Mackenzie
Journal:  Theor Appl Genet       Date:  1992-08       Impact factor: 5.699

4.  Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes.

Authors:  R A Vierling; H T Nguyen
Journal:  Theor Appl Genet       Date:  1992-09       Impact factor: 5.699

5.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

Authors:  J G Williams; A R Kubelik; K J Livak; J A Rafalski; S V Tingey
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

6.  Genetic linkage mapping in peach using morphological, RFLP and RAPD markers.

Authors:  S Rajapakse; L E Belthoff; G He; A E Estager; R Scorza; I Verde; R E Ballard; W V Baird; A Callahan; R Monet; A G Abbott
Journal:  Theor Appl Genet       Date:  1995-03       Impact factor: 5.699

7.  RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P.

Authors:  N Isabel; L Tremblay; M Michaud; F M Tremblay; J Bousquet
Journal:  Theor Appl Genet       Date:  1993-03       Impact factor: 5.699

8.  Identification and classification of celery cultivars with RAPD markers.

Authors:  X Yang; C Quiros
Journal:  Theor Appl Genet       Date:  1993-04       Impact factor: 5.699

9.  Targeted mapping and linkage analysis of morphological isozyme, and RAPD markers in peach.

Authors:  J X Chaparro; D J Werner; D O'Malley; R R Sederoff
Journal:  Theor Appl Genet       Date:  1994-02       Impact factor: 5.699

10.  Global and local genome mapping in Arabidopsis thaliana by using recombinant inbred lines and random amplified polymorphic DNAs.

Authors:  R S Reiter; J G Williams; K A Feldmann; J A Rafalski; S V Tingey; P A Scolnik
Journal:  Proc Natl Acad Sci U S A       Date:  1992-02-15       Impact factor: 11.205
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