J Arturo Prada-Oliveira1, Alonso Camacho-Ramirez2, Jesús Salas-Alvarez2, Francisco Javier Campos-Martinez2, Alfonso M Lechuga-Sancho3, David Almorza-Gomar4, Manuel Blandino-Rosano5, Gonzalo M Perez-Arana6. 1. Department of Human Anatomy and Embryology, Faculty of Medicine, University of Cádiz. Electronic address: arturo.prada@uca.es. 2. Surgery Unit, Puerto Real Hospital, University of Cádiz, Spain. 3. Department of Pediatric and Maternal Health and Radiology, Pediatric Endocrinology, Puerta del Mar Hospital, University of Cádiz, Spain. 4. Department of Operative Statistic and Research, University of Cádiz, Spain. 5. Department of Endocrinology, Diabetes and Metabolism Division, Miller School of Medicine, University of Miami, United States. 6. Department of Human Anatomy and Embryology, Faculty of Medicine, University of Cádiz.
Abstract
OBJECTIVE: The aim of this study was to clarify the role of the middle gut in the entero-pancreatic axis modification that leads to glucose improvement in the Goto-Kakizaki (GK) rat as a non-obese T2DM model. BACKGROUND: Bariatric surgery is considered an assured solution for type 2 Diabetes (T2DM). Enterohormones such as ghrelin, gastric inhibitory polypeptide and mainly glucagon-like peptide-1 (GLP-1) were recognized as key players in the physiophathological mechanisms associated with entero-pancreatic axis regulation and glucose tolerance improvement. However, the influence of anatomical arrangements post-bariatric surgery on this axis is still debatable. METHOD: To this purpose, 50% of small intestine resections were performed on GK rats (n = 6), preserving the proximal half of the jejunum and the ileum (IR50). Phenotypic and functional changes, such as performance in oral glucose tolerance tests, ileal release of GLP-1, beta-cell sensitivity to GLP-1, beta-cell mass, and turnover were characterized in IR50 and the surgical control group (Sham). RESULTS: The glucose tolerance was improved and ileal release of GLP-1 was enhanced four weeks after IR50 versus the control group rats. Beta-cell mass, beta-cell proliferation, and beta-cell sensitivity to GLP-1 were also increased in the pancreas of IR50 versus the control group rats. CONCLUSION: the jejunal exclusion increases beta-cell-mass and improves glucose tolerance by increasing in GLP-1 expression and number of receptors via the entero-pancreatic axis.
OBJECTIVE: The aim of this study was to clarify the role of the middle gut in the entero-pancreatic axis modification that leads to glucose improvement in the Goto-Kakizaki (GK) rat as a non-obese T2DM model. BACKGROUND: Bariatric surgery is considered an assured solution for type 2 Diabetes (T2DM). Enterohormones such as ghrelin, gastric inhibitory polypeptide and mainly glucagon-like peptide-1 (GLP-1) were recognized as key players in the physiophathological mechanisms associated with entero-pancreatic axis regulation and glucose tolerance improvement. However, the influence of anatomical arrangements post-bariatric surgery on this axis is still debatable. METHOD: To this purpose, 50% of small intestine resections were performed on GK rats (n = 6), preserving the proximal half of the jejunum and the ileum (IR50). Phenotypic and functional changes, such as performance in oral glucose tolerance tests, ileal release of GLP-1, beta-cell sensitivity to GLP-1, beta-cell mass, and turnover were characterized in IR50 and the surgical control group (Sham). RESULTS: The glucose tolerance was improved and ileal release of GLP-1 was enhanced four weeks after IR50 versus the control group rats. Beta-cell mass, beta-cell proliferation, and beta-cell sensitivity to GLP-1 were also increased in the pancreas of IR50 versus the control group rats. CONCLUSION: the jejunal exclusion increases beta-cell-mass and improves glucose tolerance by increasing in GLP-1 expression and number of receptors via the entero-pancreatic axis.
Authors: Camille Marciniak; Oscar Chávez-Talavera; Robert Caiazzo; Thomas Hubert; Lorea Zubiaga; Gregory Baud; Audrey Quenon; Amandine Descat; Emmanuelle Vallez; Jean François Goossens; Mostafa Kouach; Vincent Vangelder; Mathilde Gobert; Mehdi Daoudi; Bruno Derudas; Pascal Pigny; André Klein; Valéry Gmyr; Violeta Raverdy; Sophie Lestavel; Blandine Laferrère; Bart Staels; Anne Tailleux; François Pattou Journal: Am J Physiol Endocrinol Metab Date: 2021-01-25 Impact factor: 4.310