Gabriella D'Angelo1,2, Roberta Granese3, Lucia Marseglia2, Pietro Impellizzeri4, Angela Alibrandi5, Antonella Palmara2, Maria Angela La Rosa6, Sara Manti6, Carmelo Salpietro6, Edoardo Spina1, Russel J Reiter7, Raffaele Falsaperla8, Giovanni Corsello9, Eloisa Gitto2. 1. Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy. 2. Neonatal Intensive Care Unit, Gaetano Barresi Department of Human Pathology in Adult and Developmental Age, University of Messina, Messina, Italy. 3. Obstetrics and Gynecology Unit, Gaetano Barresi Department of Human Pathology in Adult and Developmental Age, University of Messina, Messina, Italy. 4. Unit of Paediatric Surgery, Gaetano Barresi Department of Human Pathology in Adult and Developmental Age, University of Messina, Messina, Italy. 5. Department of Economical, Business and Environmental Sciences and Quantitative Methods, University of Messina, Messina, Italy. 6. Unit of Paediatric Genetics and Immunology, Gaetano Barresi Department of Human Pathology in Adult and Developmental Age, University of Messina, Messina, Italy. 7. Department of Cell Systems and Anatomy, University of Texas Health Science Center, San Antonio, Texas, USA. 8. General Pediatrics and Pediatric Acute and Emergency Unit, Policlinico-Vittorio-Emanuele University Hospital, University of Catania, Catania, Italy. 9. Department of Maternal and Child Health, University of Palermo, Palermo, Italy.
Abstract
BACKGROUND: Parturition induces considerable oxidative stress and many inflammatory mediators, such as high mobility group box 1 (HMGB1), are involved from the beginning of the pregnancy to birth. The aim of the present study was to evaluate serum cord blood concentration of diacron-reactive oxygen metabolites (d-ROM), biological antioxidant potential (BAP), and HMGB1 to investigate the perinatal oxidative status of neonates and correlation with mode of delivery, as well as the influence of labor. METHODS: The subjects consisted of 214 neonates delivered at University Hospital "G. Martino", Messina, in a 6 months period. Venous blood samples were collected from the umbilical cord after cord separation. RESULTS: Umbilical cord venous blood HMGB1 was significantly higher in the spontaneous vaginal delivery (SVD) group than in the elective or emergency cesarean section (CS) group (P = 0.018). Regarding labor, there was no significant difference in HMGB1 concentration in umbilical vein blood between the spontaneous and induced labor groups (P = 0.250). Furthermore, d-ROM was significantly different between the SVD group and the elective or emergency CS group (P = 0.044). BAP concentration, however, was not significantly different, not even with regard to mode of labor. CONCLUSION: Oxidation is higher in newborns delivered by SVD than in those delivered by CS, and HMGB1 may be involved in the mechanisms of birth, and responsible for decidual modifications that lead to birth.
BACKGROUND: Parturition induces considerable oxidative stress and many inflammatory mediators, such as high mobility group box 1 (HMGB1), are involved from the beginning of the pregnancy to birth. The aim of the present study was to evaluate serum cord blood concentration of diacron-reactive oxygen metabolites (d-ROM), biological antioxidant potential (BAP), and HMGB1 to investigate the perinatal oxidative status of neonates and correlation with mode of delivery, as well as the influence of labor. METHODS: The subjects consisted of 214 neonates delivered at University Hospital "G. Martino", Messina, in a 6 months period. Venous blood samples were collected from the umbilical cord after cord separation. RESULTS: Umbilical cord venous blood HMGB1 was significantly higher in the spontaneous vaginal delivery (SVD) group than in the elective or emergency cesarean section (CS) group (P = 0.018). Regarding labor, there was no significant difference in HMGB1 concentration in umbilical vein blood between the spontaneous and induced labor groups (P = 0.250). Furthermore, d-ROM was significantly different between the SVD group and the elective or emergency CS group (P = 0.044). BAP concentration, however, was not significantly different, not even with regard to mode of labor. CONCLUSION: Oxidation is higher in newborns delivered by SVD than in those delivered by CS, and HMGB1 may be involved in the mechanisms of birth, and responsible for decidual modifications that lead to birth.
Authors: Chelsea A Saito Reis; Justin G Padron; Nainoa D Norman Ing; Claire E Kendal-Wright Journal: Am J Reprod Immunol Date: 2020-09-17 Impact factor: 3.886