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Literature DB >> 3071525

Preservation of capsular material of streptococcal cells by specific lectins determined by immunoelectron microscopy.

A Molinari¹, G Orefici, G Donelli, C Von Hunolstein, S Paradisi, G Arancia.

Abstract

We describe the use of lectins as specific stabilizing agents for the polysaccharide capsular components of two Gram-positive bacteria, Streptococcus agalactiae and Streptococcus bovis. Treatment of bacterial suspensions with wheatgerm agglutinin and concanavalin A allowed better morphological preservation as well as immunoelectron microscopic localization of a capsular component (lipoteichoic acid) by employing specific antibodies and the protein A-gold technique. Data obtained indicate that lectins are useful agents in preserving highly water-soluble capsular components during the electron microscopy procedures for both unembedded and embedded samples.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1988 PMID： 3071525 DOI： 10.1007/bf01002651

Source DB: PubMed Journal: Histochem J ISSN： 0018-2214

15 in total

1. Electron microscopic definition of surface antigens of group B Streptococcus.

Authors: D L Kasper; C J Baker
Journal: J Infect Dis Date: 1979-02 Impact factor: 5.226

2. Cell envelope morphology of rumen bacteria.

Authors: J W Costerton; H N Damgaard; K J Cheng
Journal: J Bacteriol Date: 1974-06 Impact factor: 3.490

3. Studies on the cell wall of Spirillum serpens. II. Chemical characterization of the outer structured layer.

Authors: F L Buckmire; R G Murray
Journal: Can J Microbiol Date: 1973-01 Impact factor: 2.419

4. Comparative studies on the isolation of membrane lipoteichoic acid from Lactobacillus fermenti.

Authors: A J Wicken; J W Gibbens; K W Knox
Journal: J Bacteriol Date: 1973-01 Impact factor: 3.490

Review 5. The bacterial glycocalyx in nature and disease.

Authors: J W Costerton; R T Irvin; K J Cheng
Journal: Annu Rev Microbiol Date: 1981 Impact factor: 15.500

6. Conformational aspects critical to the immunospecificity of the type III group B streptococcal polysaccharide.

Authors: H J Jennings; C Lugowski; D L Kasper
Journal: Biochemistry Date: 1981-08-04 Impact factor: 3.162

7. Immunoelectron microscopic study of the location of group-specific and type-specific polysaccharide antigens on isolated walls of group B streptococci.

Authors: M Wagner; B Wagner; V R Kubín
Journal: J Gen Microbiol Date: 1980-10

8. Kinetic and chemical analyses of the biologic significance of lipoteichoic acids in mediating adherence of serotype III group B streptococci.

Authors: T J Nealon; S J Mattingly
Journal: Infect Immun Date: 1985-10 Impact factor: 3.441

Preservation of capsular material of streptococcal cells by specific lectins determined by immunoelectron microscopy.

1. Electron microscopic definition of surface antigens of group B Streptococcus.

2. Cell envelope morphology of rumen bacteria.

3. Studies on the cell wall of Spirillum serpens. II. Chemical characterization of the outer structured layer.

4. Comparative studies on the isolation of membrane lipoteichoic acid from Lactobacillus fermenti.

Review 5. The bacterial glycocalyx in nature and disease.

6. Conformational aspects critical to the immunospecificity of the type III group B streptococcal polysaccharide.

7. Immunoelectron microscopic study of the location of group-specific and type-specific polysaccharide antigens on isolated walls of group B streptococci.

8. Kinetic and chemical analyses of the biologic significance of lipoteichoic acids in mediating adherence of serotype III group B streptococci.

9. Epithelial cell binding of group A streptococci by lipoteichoic acid on fimbriae denuded of M protein.

10. Protection of mice from experimental infection with type III group B Streptococcus using monoclonal antibodies.

1. Ultrastructural study of surface components of Streptococcus suis.

2. First Report on the Streptococcusgallolyticus (S. bovis Biotype I) DSM 13808 Exopolysaccharide Structure.