| Literature DB >> 30710280 |
Natalia Kozlyuk1,2, Andrew J Monteith3, Velia Garcia2,4, Steven M Damo1,2,5, Eric P Skaar3, Walter J Chazin6,7,8.
Abstract
S100 proteins are distinct dimeric EF-hand Ca2+-binding proteins that can bind Zn2+, Mn2+, and other transition metals with high affinity at two sites in the dimer interface. Certain S100 proteins, including S100A7, S100A12, S100A8, and S100A9, play key roles in the innate immune response to pathogens. These proteins function via a "nutritional immunity" mechanism by depleting essential transition metals in the infection that are required for the invading organism to grow and thrive. They also act as damage-associated molecular pattern ligands, which activate pattern recognition receptors (e.g., Toll-like receptor 4, RAGE) that mediate inflammation. Here we present protocols for these S100 proteins for high-level production of recombinant protein, measurement of binding affinities using isothermal titration calorimetry, and an assay of antimicrobial activity.Entities:
Keywords: Antimicrobial growth assay; Calprotectin; Host-pathogen interaction; Inflammatory response; Isothermal titration calorimetry; Metal binding; Nutritional immunity; Protein expression; Protein purification; S100 proteins; S100A12; S100A7; S100A8; S100A9
Mesh：
Substances：
Year: 2019 PMID： 30710280 PMCID： PMC6475579 DOI： 10.1007/978-1-4939-9030-6_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745