OBJECTIVE: Explore changes in micro-RNA (miRNA) expression in blood after sport-related concussion (SRC) in collegiate athletes. METHODS: Twenty-seven collegiate athletes (~41% male, ~75% white, age 18.8 ± 0.8 years) provided both baseline and post-SRC blood samples. Serum was analyzed for expression of miR-153-3p (n = 27), miR-223-3p (n = 23), miR-26a-5p (n = 26), miR-423-3p (n = 23), and miR-let-7a-5p (n = 23) at both time points via quantitative polymerase chain reaction (qPCR). Nonparametric analyses were used to compare miRNA expression changes between baseline and SRC and to evaluate associations with clinical outcomes (symptom severity, cognition, balance, and oculomotor function, and clinical recovery time). RESULTS: Participants manifested a significant increase in miRNA expression following SRC for miR153-3p (Z = -2.180, p = .029, 59% of the participants increased post-SRC), miR223-3p (Z = -1.998, p = .046, 70% increased), and miR-let-7a-5p (Z = -2.190, p = .029, 65% increased). There were no statistically significant associations between changes in miRNA expression and clinical test scores, acute symptom severity, or clinical recovery time. CONCLUSION: MiR-153-3p, miR-223-3p, and miR-let-7a-5p were significantly upregulated acutely following SRC in male and female collegiate athletes compared to baseline levels, though several athletes demonstrated no change or a decrease in expression. The biological mechanisms and functional implications of the increased expression of these circulating miRNA are unclear and require more research, as does their relevance to clinical outcomes.
OBJECTIVE: Explore changes in micro-RNA (miRNA) expression in blood after sport-related concussion (SRC) in collegiate athletes. METHODS: Twenty-seven collegiate athletes (~41% male, ~75% white, age 18.8 ± 0.8 years) provided both baseline and post-SRC blood samples. Serum was analyzed for expression of miR-153-3p (n = 27), miR-223-3p (n = 23), miR-26a-5p (n = 26), miR-423-3p (n = 23), and miR-let-7a-5p (n = 23) at both time points via quantitative polymerase chain reaction (qPCR). Nonparametric analyses were used to compare miRNA expression changes between baseline and SRC and to evaluate associations with clinical outcomes (symptom severity, cognition, balance, and oculomotor function, and clinical recovery time). RESULTS:Participants manifested a significant increase in miRNA expression following SRC for miR153-3p (Z = -2.180, p = .029, 59% of the participants increased post-SRC), miR223-3p (Z = -1.998, p = .046, 70% increased), and miR-let-7a-5p (Z = -2.190, p = .029, 65% increased). There were no statistically significant associations between changes in miRNA expression and clinical test scores, acute symptom severity, or clinical recovery time. CONCLUSION:MiR-153-3p, miR-223-3p, and miR-let-7a-5p were significantly upregulated acutely following SRC in male and female collegiate athletes compared to baseline levels, though several athletes demonstrated no change or a decrease in expression. The biological mechanisms and functional implications of the increased expression of these circulating miRNA are unclear and require more research, as does their relevance to clinical outcomes.
Authors: Paul McCrory; Willem Meeuwisse; Jiří Dvořák; Mark Aubry; Julian Bailes; Steven Broglio; Robert C Cantu; David Cassidy; Ruben J Echemendia; Rudy J Castellani; Gavin A Davis; Richard Ellenbogen; Carolyn Emery; Lars Engebretsen; Nina Feddermann-Demont; Christopher C Giza; Kevin M Guskiewicz; Stanley Herring; Grant L Iverson; Karen M Johnston; James Kissick; Jeffrey Kutcher; John J Leddy; David Maddocks; Michael Makdissi; Geoff T Manley; Michael McCrea; William P Meehan; Shinji Nagahiro; Jon Patricios; Margot Putukian; Kathryn J Schneider; Allen Sills; Charles H Tator; Michael Turner; Pieter E Vos Journal: Br J Sports Med Date: 2017-04-26 Impact factor: 13.800
Authors: Wang-Xia Wang; Nishant P Visavadiya; Jignesh D Pandya; Peter T Nelson; Patrick G Sullivan; Joe E Springer Journal: Exp Neurol Date: 2015-01-03 Impact factor: 5.330
Authors: Tanja Kunej; Irena Godnic; Jana Ferdin; Simon Horvat; Peter Dovc; George Adrian Calin Journal: Mutat Res Date: 2011-03-21 Impact factor: 2.433
Authors: Biswadev Mitra; Thomas F Rau; Nanda Surendran; James H Brennan; Prasanthan Thaveenthiran; Edmond Sorich; Mark C Fitzgerald; Jeffrey V Rosenfeld; Sarjubhai A Patel Journal: J Clin Neurosci Date: 2017-01-20 Impact factor: 1.961
Authors: Jonathan B Johnnidis; Marian H Harris; Robert T Wheeler; Sandra Stehling-Sun; Michael H Lam; Oktay Kirak; Thijn R Brummelkamp; Mark D Fleming; Fernando D Camargo Journal: Nature Date: 2008-02-17 Impact factor: 49.962
Authors: Valerie B Sampson; Nancy H Rong; Jian Han; Qunying Yang; Virginie Aris; Patricia Soteropoulos; Nicholas J Petrelli; Stephen P Dunn; Leslie J Krueger Journal: Cancer Res Date: 2007-10-15 Impact factor: 12.701
Authors: Eric R Muñoz; Jaclyn B Caccese; Brittany E Wilson; Kyle T Shuler; Fernando V Santos; Carolina T Cabán; John J Jeka; Dianne Langford; Matthew B Hudson Journal: J Sport Health Sci Date: 2020-11-12 Impact factor: 7.179
Authors: Sandy R Shultz; Caroline J Taylor; Riemke Aggio-Bruce; William T O'Brien; Mujun Sun; Adrian V Cioanca; George Neocleous; Georgia F Symons; Rhys D Brady; Anandwardhan A Hardikar; Mugdha V Joglekar; Daniel M Costello; Terence J O'Brien; Riccardo Natoli; Stuart J McDonald Journal: Biomark Insights Date: 2022-02-28
Authors: Maciej Tarnowski; Patrycja Tomasiak; Marta Tkacz; Katarzyna Zgutka; Katarzyna Piotrowska Journal: Genes (Basel) Date: 2022-08-17 Impact factor: 4.141