| Literature DB >> 30703551 |
Ivan Fan Xia1, Jacky St Cheung1, Manhui Wu1, Kwong-Sen Wong1, Hang-Kin Kong1, Xiao-Ting Zheng2, Ka-Hing Wong3, Kevin Wh Kwok4.
Abstract
Selenium (Se) is an essential micronutrient for human and animals. It plays an important role in antioxidative stress, selenoenzymes regulation and immunomodulation. In this study, two common immunostimulants chitosan (CTS) and Se were used to synthesize nanoparticles (CTS-SeNP). Immunomodulation of CTS-SeNP were explored in wild-type zebrafish (Danio rerio). Dietary supplementation of CTS-SeNP enhanced lysozyme activity, phagocytic respiratory burst as well as splenocytes proliferation stimulated by LPS and ConA. CTS-SeNP showed immunomodulation effect from 5 to 20 μg/g but the best outcome was observed at 10 μg/g. Immunomodulation effect were rapidly induced after 3-9d and can sustain to 60. The zebrafish fed with 10 μg/g CTS-SeNP also showed 26.7% higher survival rate than the control after intraperitoneal injection of common bacterium Aeromonas hydrophila. Our results suggested that CTS-SeNP is an effective immunostimulant to fish and has potential application in aquaculture.Entities:
Keywords: Bacteria infection; Combined material; Immunostimulant; Nanoparticles
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Year: 2019 PMID： 30703551 DOI： 10.1016/j.fsi.2019.01.042
Source DB: PubMed Journal: Fish Shellfish Immunol ISSN： 1050-4648 Impact factor: 4.581