Jean-Luc Da Lage1, Gregg W C Thomas2, Magalie Bonneau3, Virginie Courtier-Orgogozo4. 1. UMR 9191 Évolution, Génomes, Comportement, Écologie. CNRS, IRD, Université Paris-Sud. Université Paris-Saclay, F-91198, Gif-sur-Yvette, France. jean-luc.da-lage@egce.cnrs-gif.fr. 2. Department of Biology and Department of Computer Science, Indiana University, Bloomington, IN, 47405, USA. 3. UMR 9191 Évolution, Génomes, Comportement, Écologie. CNRS, IRD, Université Paris-Sud. Université Paris-Saclay, F-91198, Gif-sur-Yvette, France. 4. Institut Jacques Monod-CNRS UMR7592-Université Paris Diderot, 15 rue Hélène Brion, 75013, Paris, France.
Abstract
BACKGROUND: At the very end of the larval stage Drosophila expectorate a glue secreted by their salivary glands to attach themselves to a substrate while pupariating. The glue is a mixture of apparently unrelated proteins, some of which are highly glycosylated and possess internal repeats. Because species adhere to distinct substrates (i.e. leaves, wood, rotten fruits), glue genes are expected to evolve rapidly. RESULTS: We used available genome sequences and PCR-sequencing of regions of interest to investigate the glue genes in 20 Drosophila species. We discovered a new gene in addition to the seven glue genes annotated in D. melanogaster. We also identified a phase 1 intron at a conserved position present in five of the eight glue genes of D. melanogaster, suggesting a common origin for those glue genes. A slightly significant rate of gene turnover was inferred. Both the number of repeats and the repeat sequence were found to diverge rapidly, even between closely related species. We also detected high repeat number variation at the intrapopulation level in D. melanogaster. CONCLUSION: Most conspicuous signs of accelerated evolution are found in the repeat regions of several glue genes.
BACKGROUND: At the very end of the larval stage Drosophila expectorate a glue secreted by their salivary glands to attach themselves to a substrate while pupariating. The glue is a mixture of apparently unrelated proteins, some of which are highly glycosylated and possess internal repeats. Because species adhere to distinct substrates (i.e. leaves, wood, rotten fruits), glue genes are expected to evolve rapidly. RESULTS: We used available genome sequences and PCR-sequencing of regions of interest to investigate the glue genes in 20 Drosophila species. We discovered a new gene in addition to the seven glue genes annotated in D. melanogaster. We also identified a phase 1 intron at a conserved position present in five of the eight glue genes of D. melanogaster, suggesting a common origin for those glue genes. A slightly significant rate of gene turnover was inferred. Both the number of repeats and the repeat sequence were found to diverge rapidly, even between closely related species. We also detected high repeat number variation at the intrapopulation level in D. melanogaster. CONCLUSION: Most conspicuous signs of accelerated evolution are found in the repeat regions of several glue genes.
Authors: Cheng Sun; Jiaxing Huang; Yun Wang; Xiaomeng Zhao; Long Su; Gregg W C Thomas; Mengya Zhao; Xingtan Zhang; Irwin Jungreis; Manolis Kellis; Saverio Vicario; Igor V Sharakhov; Semen M Bondarenko; Martin Hasselmann; Chang N Kim; Benedict Paten; Luca Penso-Dolfin; Li Wang; Yuxiao Chang; Qiang Gao; Ling Ma; Lina Ma; Zhang Zhang; Hongbo Zhang; Huahao Zhang; Livio Ruzzante; Hugh M Robertson; Yihui Zhu; Yanjie Liu; Huipeng Yang; Lele Ding; Quangui Wang; Dongna Ma; Weilin Xu; Cheng Liang; Michael W Itgen; Lauren Mee; Gang Cao; Ze Zhang; Ben M Sadd; Matthew W Hahn; Sarah Schaack; Seth M Barribeau; Paul H Williams; Robert M Waterhouse; Rachel Lockridge Mueller Journal: Mol Biol Evol Date: 2021-01-23 Impact factor: 16.240
Authors: Marta Maria Antoniazzi; Pedro Luiz Mailho-Fontana; Fausto Nomura; Heloisa Bastianon Azevedo; Daniel Carvalho Pimenta; Juliana Mozer Sciani; Fernando Rogério Carvalho; Denise Cerqueira Rossa-Feres; Carlos Jared Journal: iScience Date: 2022-03-16