Wei Tang1, Xiaoshen Zhang1, Weige Tan1, Jin Gao1, Lingxiao Pan1, Xigang Ye1, Lun Chen1, Wenbo Zheng2. 1. Department of Breast Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China. 2. Department of Breast Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, People's Republic of China. Electronic address: wenbo_zheng@163.com.
Abstract
BACKGROUND: In this study, we aimed to investigate the expression and clinical significance of miR-145-5p and its tumor-suppressive effect in breast cancer patients. METHODS: We used luciferase reporter assay, real-time quantitative reverse transcription polymerase chain reaction and Western blot to identify sex-determining region Y-box2 (SOX2) as the target gene of miR-145-5p. Their expression levels in breast cancer tissues (n = 122) were detected by real-time quantitative polymerase chain reaction. We also applied 3-(4,5-dimethyl- 2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay and flow cytometry to reveal the effect of miR-145-5p on proliferation in breast cancer. RESULTS: miR-145-5p expression is downregulated in breast cancer tissues and negatively correlated with SOX2 expression. Decreased miR-145-5p expression was significantly associated with larger tumor size, distal metastasis, higher Ki67 expression level, and shorter overall survival. miR-145-5p inhibits breast cancer cell proliferation via targeting SOX2, and multivariate regression showed that both miR-145-5p and SOX2 were unfavorable prognostic factors. CONCLUSIONS: miR-145-5p played a suppressive role in the proliferation of breast cancer cells by targeting SOX2, and miR-145-5p is a putative biomarker for risk assessment in patients with breast cancer.
BACKGROUND: In this study, we aimed to investigate the expression and clinical significance of miR-145-5p and its tumor-suppressive effect in breast cancerpatients. METHODS: We used luciferase reporter assay, real-time quantitative reverse transcription polymerase chain reaction and Western blot to identify sex-determining region Y-box2 (SOX2) as the target gene of miR-145-5p. Their expression levels in breast cancer tissues (n = 122) were detected by real-time quantitative polymerase chain reaction. We also applied 3-(4,5-dimethyl- 2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay and flow cytometry to reveal the effect of miR-145-5p on proliferation in breast cancer. RESULTS:miR-145-5p expression is downregulated in breast cancer tissues and negatively correlated with SOX2 expression. Decreased miR-145-5p expression was significantly associated with larger tumor size, distal metastasis, higher Ki67 expression level, and shorter overall survival. miR-145-5p inhibits breast cancer cell proliferation via targeting SOX2, and multivariate regression showed that both miR-145-5p and SOX2 were unfavorable prognostic factors. CONCLUSIONS:miR-145-5p played a suppressive role in the proliferation of breast cancer cells by targeting SOX2, and miR-145-5p is a putative biomarker for risk assessment in patients with breast cancer.
Authors: Danielle Pessôa-Pereira; Adriane Feijó Evangelista; Rhafaela Lima Causin; René Aloisio da Costa Vieira; Lucas Faria Abrahão-Machado; Iara Viana Vidigal Santana; Vinicius Duval da Silva; Karen Cristina Borba de Souza; Renato José de Oliveira-Silva; Gabriela Carvalho Fernandes; Rui Manuel Reis; Edenir Inêz Palmero; Márcia Maria Chiquitelli Marques Journal: BMC Cancer Date: 2020-02-22 Impact factor: 4.430