Literature DB >> 30688315

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures.

Felipe J Núñez1, Flor M Mendez2, Maria B Garcia-Fabiani1, Joaquín Pardo3, Marta Edwards1, Pedro R Lowenstein1, Maria G Castro4.   

Abstract

Analysis of protein expression in glioma is relevant for several aspects in the study of its pathology. Numerous proteins have been described as biomarkers with applications in diagnosis, prognosis, classification, state of tumor progression, and cell differentiation state. These analyses of biomarkers are also useful to characterize tumor neurospheres (NS) generated from glioma patients and glioma models. Tumor NS provide a valuable in vitro model to assess different features of the tumor from which they are derived and can more accurately mirror glioma biology. Here we describe a detailed method to analyze biomarkers in tumor NS using immunohistochemistry (IHC) on paraffin-embedded tumor NS.

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Year:  2019        PMID： 30688315      PMCID： PMC6352905          DOI： 10.3791/58931

Source DB:  PubMed          Journal:  J Vis Exp        ISSN： 1940-087X            Impact factor:   1.355


  21 in total

1.  Preparation of cells for microscopy using cytospin.

Authors:  Cheryl M Koh
Journal:  Methods Enzymol       Date:  2013       Impact factor: 1.600

2.  Transposon mediated integration of plasmid DNA into the subventricular zone of neonatal mice to generate novel models of glioblastoma.

Authors:  Anda-Alexandra Calinescu; Felipe Javier Núñez; Carl Koschmann; Bradley L Kolb; Pedro R Lowenstein; Maria G Castro
Journal:  J Vis Exp       Date:  2015-02-22       Impact factor: 1.355

3.  Antigen retrieval techniques in immunohistochemistry: comparison of different methods.

Authors:  S A Pileri; G Roncador; C Ceccarelli; M Piccioli; A Briskomatis; E Sabattini; S Ascani; D Santini; P P Piccaluga; O Leone; S Damiani; C Ercolessi; F Sandri; F Pieri; L Leoncini; B Falini
Journal:  J Pathol       Date:  1997-09       Impact factor: 7.996

4.  Identification of OLIG2 as the most specific glioblastoma stem cell marker starting from comparative analysis of data from similar DNA chip microarray platforms.

Authors:  Anne-Laure Trépant; Christelle Bouchart; Sandrine Rorive; Sébastien Sauvage; Christine Decaestecker; Pieter Demetter; Isabelle Salmon
Journal:  Tumour Biol       Date:  2014-11-12

5.  De novo induction of genetically engineered brain tumors in mice using plasmid DNA.

Authors:  Stephen M Wiesner; Stacy A Decker; Jon D Larson; Katya Ericson; Colleen Forster; Jose L Gallardo; Chunmei Long; Zachary L Demorest; Edward A Zamora; Walter C Low; Karen SantaCruz; David A Largaespada; John R Ohlfest
Journal:  Cancer Res       Date:  2009-01-15       Impact factor: 12.701

Review 6.  Brain tumor stem cells: bringing order to the chaos of brain cancer.

Authors:  Peter B Dirks
Journal:  J Clin Oncol       Date:  2008-06-10       Impact factor: 44.544

7.  Ki67 index in intracranial ependymoma: a promising histopathological candidate biomarker.

Authors:  M Preusser; H Heinzl; E Gelpi; R Höftberger; I Fischer; I Pipp; I Milenkovic; A Wöhrer; F Popovici; S Wolfsberger; J A Hainfellner
Journal:  Histopathology       Date:  2008-07       Impact factor: 5.087

Review 8.  Personalized medicine using DNA biomarkers: a review.

Authors:  Andreas Ziegler; Armin Koch; Katja Krockenberger; Anika Grosshennig
Journal:  Hum Genet       Date:  2012-07-01       Impact factor: 4.132

9.  The Human Glioblastoma Cell Culture Resource: Validated Cell Models Representing All Molecular Subtypes.

Authors:  Yuan Xie; Tobias Bergström; Yiwen Jiang; Patrik Johansson; Voichita Dana Marinescu; Nanna Lindberg; Anna Segerman; Grzegorz Wicher; Mia Niklasson; Sathishkumar Baskaran; Smitha Sreedharan; Isabelle Everlien; Marianne Kastemar; Annika Hermansson; Lioudmila Elfineh; Sylwia Libard; Eric Charles Holland; Göran Hesselager; Irina Alafuzoff; Bengt Westermark; Sven Nelander; Karin Forsberg-Nilsson; Lene Uhrbom
Journal:  EBioMedicine       Date:  2015-08-15       Impact factor: 8.143

Review 10.  Biomarkers of apoptosis.

Authors:  T H Ward; J Cummings; E Dean; A Greystoke; J M Hou; A Backen; M Ranson; C Dive
Journal:  Br J Cancer       Date:  2008-09-16       Impact factor: 7.640
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  1 in total

1.  Therapeutic Efficacy of Immune Stimulatory Thymidine Kinase and fms-like Tyrosine Kinase 3 Ligand (TK/Flt3L) Gene Therapy in a Mouse Model of High-Grade Brainstem Glioma.

Authors:  Flor Mendez; Padma Kadiyala; Felipe J Nunez; Stephen Carney; Fernando M Nunez; Jessica C Gauss; Ramya Ravindran; Sheeba Pawar; Marta Edwards; Maria Belen Garcia-Fabiani; Santiago Haase; Pedro R Lowenstein; Maria G Castro
Journal:  Clin Cancer Res       Date:  2020-04-24       Impact factor: 12.531
  1 in total

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