Ľubomíra Tóthová1,2, Peter Celec1,3,4, Imrich Mucska5, Július Hodosy6,7,8. 1. Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, 841 01, Bratislava, Slovakia. 2. Institute of Physiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia. 3. Institute of Pathophysiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia. 4. Department of Molecular Biology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia. 5. University Hospital, Bratislava, Slovakia. 6. Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Sasinkova 4, 841 01, Bratislava, Slovakia. hodosy@gmail.com. 7. Institute of Physiology, Faculty of Medicine, Comenius University, Bratislava, Slovakia. hodosy@gmail.com. 8. University Hospital, Bratislava, Slovakia. hodosy@gmail.com.
Abstract
PURPOSE: Obstructive sleep apnea (OSA) is associated with oxidative stress that is involved in the pathogenesis of cardiovascular and metabolic complications. The concentrations of salivary markers of oxidative stress in patients with OSA increase considerably during the night. The dynamics is not affected by continuous positive airway pressure (CPAP) in mild to moderate OSA. The aim of this study was to analyze the short-term effects of CPAP on salivary oxidative stress markers in patients with severe OSA. METHODS: Salivary samples were collected from 24 patients with apnea-hypopnea index higher than 30 during the first (diagnostic) night, who were treated by CPAP during the second (therapeutic) night. RESULTS: The salivary markers of oxidative stress (TBARS, AGEs, and AOPP) were higher in the morning after the diagnostic night when compared to the evening concentrations (p < 0.01 for TBARS and p < 0.05 for AGEs and AOPP). Treatment by CPAP significantly decreased the morning concentrations of TBARS, AOPP (p < 0.01 for both), and AGEs (p < 0.05). Also, TBARS and AGEs positively correlated with apnea-hypopnea index (r = 0.48 and 0.49, respectively; p < 0.05). Antioxidant statuss was not affected. CONCLUSION: Severe OSA is associated with increased levels of saliva markers for lipid peroxidation, protein oxidative damage, and carbonyl damage. Even short-term CPAP partially prevents oxidative and carbonyl stress during the night and this can be monitored non-invasively using saliva.
PURPOSE: Obstructive sleep apnea (OSA) is associated with oxidative stress that is involved in the pathogenesis of cardiovascular and metabolic complications. The concentrations of salivary markers of oxidative stress in patients with OSA increase considerably during the night. The dynamics is not affected by continuous positive airway pressure (CPAP) in mild to moderate OSA. The aim of this study was to analyze the short-term effects of CPAP on salivary oxidative stress markers in patients with severe OSA. METHODS: Salivary samples were collected from 24 patients with apnea-hypopnea index higher than 30 during the first (diagnostic) night, who were treated by CPAP during the second (therapeutic) night. RESULTS: The salivary markers of oxidative stress (TBARS, AGEs, and AOPP) were higher in the morning after the diagnostic night when compared to the evening concentrations (p < 0.01 for TBARS and p < 0.05 for AGEs and AOPP). Treatment by CPAP significantly decreased the morning concentrations of TBARS, AOPP (p < 0.01 for both), and AGEs (p < 0.05). Also, TBARS and AGEs positively correlated with apnea-hypopnea index (r = 0.48 and 0.49, respectively; p < 0.05). Antioxidant statuss was not affected. CONCLUSION: Severe OSA is associated with increased levels of saliva markers for lipid peroxidation, protein oxidative damage, and carbonyl damage. Even short-term CPAP partially prevents oxidative and carbonyl stress during the night and this can be monitored non-invasively using saliva.
Authors: Peter Celec; Július Hodosy; Michal Behuliak; Roland Pálffy; Roman Gardlík; Lukáč Halčák; Imrich Mucska Journal: Sleep Breath Date: 2011-03-26 Impact factor: 2.816