| Literature DB >> 30685237 |
Deshui Yu1, Ju Zhang1, Guangxuan Tan1, Ningshu Yu2, Qiuyue Wang2, Qiqi Duan2, Xin Qi2, Mingjiao Cheng2, Chunxue Yan2, Zhangkun Wei2, Zhenmiao Yu3, Wenchao Huang3, Chengwei Li4.
Abstract
Genomic DNA isolation is a crucial technique for researchers studying plant molecular biology. A current widely-used protocol for DNA extraction needs a pestle and mortal for each sample and consumes a large amount of liquid nitrogen in grinding the samples. Most high-throughput methods depend on expensive machines for tissue homogenization. Here we developed a CTAB-based DNA extraction method using 2.0 mL microcentrifuge tubes for sample processing. This protocol has the advantages that it is suitable for a variety of plants, easily-performed without special equipment, and high-throughput; it effectively avoids sample cross-contamination, and is inexpensive, rapid and safe.Entities:
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Year: 2019 PMID： 30685237 DOI： 10.1016/j.ab.2019.01.007
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365