Analytical methodology for studying cellular uptake, processing and localization of gold nanoparticles.

Interactions of gold nanoparticles (AuNPs) with live cells are known to exert a great impact on their functions, including cell signalling, genomic, proteomic, and metabolomic processes. Modern analytical techniques applied to studying nanoparticle-cell interactions are to improve our understanding of the mode of action of AuNPs, which is essential for their approval in disease therapeutics. Such methods may vary depending on what step of particle internalization is in question, i.e., cellular uptake, intracellular transport (accompanying by changes in the chemical state), translocation to different cell compartments, interaction with relevant subcellular structures and localization. This review focuses on the implementation and critical assessment of advanced analytical methodologies to investigate the cellular processing of AuNPs. Also addressed is a sought-after issue of accounting in in-vitro studies for a chemical form in which the AuNPs enter the cell in vivo.