Literature DB >> 3068257

Rapid identification of Escherichia coli by Fluorocult media and positive indole reaction.

W Heizmann1, P C Döller, B Gutbrod, H Werner.   

Abstract

To assess the specificity and sensitivity of Fluorocult media for the identification of Escherichia coli, the beta-glucuronidase activities of 1,258 bacterial strains, as well as 20 strains of Candida spp., were investigated. Fluorescence of colonies combined with positive indole reaction resulted in specificities of 99.6 to 99.8%. Sensitivities were 59.1% (MacConkey agar), 69.9% (brolacin agar), 85.5% (Columbia agar), and 85.8% (ECD agar).

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Year:  1988        PMID: 3068257      PMCID: PMC266976          DOI: 10.1128/jcm.26.12.2682-2684.1988

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  9 in total

1.  Rapid diagnosis of Enterobacteriaceae. I. Detection of bacterial glycosidases.

Authors:  M Kilian; P Bülow
Journal:  Acta Pathol Microbiol Scand B       Date:  1976-10

2.  [Correlation between beta-glucuronidase activity and serotype in the genus "Salmonella" (author's transl)].

Authors:  L Le Minor; J Buissière; G Novel; M Novel
Journal:  Ann Microbiol (Paris)       Date:  1978 Aug-Sep

3.  Benefit and advantage to clinicians of a computer-assisted microbiological diagnosis and database system.

Authors:  K Manncke; W Heizmann
Journal:  Infection       Date:  1988       Impact factor: 3.553

4.  Escherichia coli infections.

Authors:  M M Levine
Journal:  N Engl J Med       Date:  1985-08-15       Impact factor: 91.245

Review 5.  Biochemical identification of new species and biogroups of Enterobacteriaceae isolated from clinical specimens.

Authors:  J J Farmer; B R Davis; F W Hickman-Brenner; A McWhorter; G P Huntley-Carter; M A Asbury; C Riddle; H G Wathen-Grady; C Elias; G R Fanning
Journal:  J Clin Microbiol       Date:  1985-01       Impact factor: 5.948

6.  Fluorogenic assays for immediate confirmation of Escherichia coli.

Authors:  P C Feng; P A Hartman
Journal:  Appl Environ Microbiol       Date:  1982-06       Impact factor: 4.792

7.  Comparison of beta-glucuronidase-based substrate systems for identification of Escherichia coli.

Authors:  S C Edberg; C M Kontnick
Journal:  J Clin Microbiol       Date:  1986-09       Impact factor: 5.948

8.  Methylumbelliferyl-beta-D-glucuronide-based medium for rapid isolation and identification of Escherichia coli.

Authors:  R W Trepeta; S C Edberg
Journal:  J Clin Microbiol       Date:  1984-02       Impact factor: 5.948

9.  Detection of beta-glucuronidase in lactose-fermenting members of the family Enterobacteriaceae and its presence in bacterial urine cultures.

Authors:  W Hansen; E Yourassowsky
Journal:  J Clin Microbiol       Date:  1984-12       Impact factor: 5.948

  9 in total
  14 in total

1.  Cost-effective and rapid presumptive identification of gram-negative bacilli in routine urine, pus, and stool cultures: evaluation of the use of CHROMagar orientation medium in conjunction with simple biochemical tests.

Authors:  K Ohkusu
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

2.  Fluorogenic substrates for differentiation of gram-negative nonfermentative and oxidase-positive fermentative bacteria.

Authors:  P Kämpfer; I Kulies; W Dott
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

3.  Glycosidase profiles of members of the family Enterobacteriaceae.

Authors:  P Kämpfer; O Rauhoff; W Dott
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948

Review 4.  Fluorogenic and chromogenic substrates used in bacterial diagnostics.

Authors:  M Manafi; W Kneifel; S Bascomb
Journal:  Microbiol Rev       Date:  1991-09

5.  Evaluation of CPS ID2 medium for diagnosis of urinary infections.

Authors:  M L Núñez; J Díaz; I Lorente; J Pérez; J Ruiz
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1995-12       Impact factor: 3.267

6.  Evaluation of BBL CHROMagar orientation medium for detection and presumptive identification of urinary tract pathogens.

Authors:  K A Hengstler; R Hammann; A M Fahr
Journal:  J Clin Microbiol       Date:  1997-11       Impact factor: 5.948

7.  Mucosally-directed adrenergic nerves and sympathomimetic drugs enhance non-intimate adherence of Escherichia coli O157:H7 to porcine cecum and colon.

Authors:  Chunsheng Chen; Mark Lyte; Mark P Stevens; Lucy Vulchanova; David R Brown
Journal:  Eur J Pharmacol       Date:  2006-04-06       Impact factor: 4.432

8.  Evaluation of the DipStreak, a new device with an original streaking mechanism for detection, counting, and presumptive identification of urinary tract pathogens.

Authors:  Claudio Scarparo; Paola Piccoli; Paolo Ricordi; Mariuccia Scagnelli
Journal:  J Clin Microbiol       Date:  2002-06       Impact factor: 5.948

9.  Rapid biochemical test to identify verocytotoxin-positive strains of Escherichia coli serotype O157.

Authors:  J S Thompson; D S Hodge; A A Borczyk
Journal:  J Clin Microbiol       Date:  1990-10       Impact factor: 5.948

10.  Evaluation of CPS ID2 medium for detection of urinary tract bacterial isolates in specimens from a rehabilitation center.

Authors:  M A Mazoyer; S Orenga; F Doleans; J Freney
Journal:  J Clin Microbiol       Date:  1995-04       Impact factor: 5.948

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