| Literature DB >> 30681406 |
Yoshikazu Furusawa1,2,3, Shinji Yamada1, Shunsuke Itai1,4, Masato Sano1, Takuro Nakamura1, Miyuki Yanaka1, Masato Fukui3, Hiroyuki Harada4, Takuya Mizuno5, Yusuke Sakai6, Masaki Takasu7, Mika K Kaneko1, Yukinari Kato1,2.
Abstract
Podoplanin (PDPN) is a type I transmembrane glycoprotein that is expressed in normal tissues, including renal corpuscles and type I lung alveolar cells. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine PDPNs have already been established; however, antipig PDPN (pPDPN) mAbs have not. We therefore immunized mice with pPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/pPDPN), and screened hybridomas, which are producing anti-pPDPN mAbs. One of mAbs, PMab-210 (an IgG1, kappa), was able to specifically detect CHO/pPDPN cells by flow cytometry and detect pPDPN by Western blot analysis. Furthermore, PMab-210 strongly stained type I lung alveolar cells and weakly stained renal corpuscles by immunohistochemistry. PMab-210 is expected to be useful in investigating the function of pPDPN.Entities:
Keywords: PDPN; PMab-210; pig podoplanin
Mesh：
Substances：
Year: 2019 PMID： 30681406 DOI： 10.1089/mab.2018.0038
Source DB: PubMed Journal: Monoclon Antib Immunodiagn Immunother ISSN： 2167-9436