Employing AgNPs doped amidoxime-modified polyacrylonitrile (PAN-oxime) nanofibers for target induced strand displacement-based electrochemical aptasensing of CA125 in ovarian cancer patients.

In this study, a high-performance biosensing nanoplatform based on amidoxime-modified polyacrylonitrile nanofibers decorated with Ag nanoparticles (AgNPs-PAN-oxime NFs) is described. The AgNPs-PAN-oxime NFs were prepared by the combination of electrospinning technique and chemical modification of nitrile group in the PAN. The proposed signal amplifiying nanoplatform was applied in the fabrication of an electrochemical aptasensor for the sensitive detection of CA 125 based on aptamer-cDNA duplex and target induced strand displacement recognition mechanism. The aptasensing interface offers high sensitivity and selectivity for detection of tumor marker due to inherent advantages such as high specific surface area of NFs, good conductivity by doping AgNPs into the polymer NFs and especially the ideal selectivity of anti CA 125 aptamer to its target. The electrochemical aptasensor revealed a wide dynamic linear range (DLR) from 0.01 to 350 U mL-1 with a correlation coefficient of 0.991 and limit of detection (LOD) of 0.0042 U mL-1. Additionally, the designed aptasensor showed acceptable selectivity, reproducibility, repeatability and stability. The satisfactory results for determination of CA 125 in serum samples compared to ELISA method (p-value > 0.05) indicated the potential application of aptasensor in clinical monitoring of tumor biomarker for early diagnosis and management of ovarian cancer. Published by Elsevier B.V.