miR-7-5p inhibits cell migration and invasion in glioblastoma through targeting SATB1.

MicroRNAs (miRNAs/miRs) have been revealed to influence the development and progression of glioblastoma. Although a number of miRNAs are abnormally expressed in glioblastoma it is not clear whether they are a factor associated with glioblastoma pathogenesis. In the present study, miR-7-5p was identified as being aberrantly downregulated in glioblastoma tissues and cell lines. miR-7-5p overexpression significantly decreased the migratory and invasive capacity of the cells, while miR-7-5p silencing had the opposite effect. In addition, a luciferase assay confirmed that special AT rich sequence binding protein 1 (SATB1) was a direct target gene of miR-7-5p in glioblastoma. The overexpression of SATB1 in glioblastoma was revealed to promote cell migration and invasion. In addition, SATB1 overexpression may weaken the inhibitory effect of miR-7-5p on cell migration and invasion. miR-7-5p overexpression reversed the effects of SATB1 on cell migration and invasion in glioblastoma cells. In conclusion, miR-7-5p may be a useful therapeutic target for the diagnosis and treatment of patients with glioblastoma.

Introduction

Glioblastoma is a common primary tumor in the central nervous system, with a 5-year survival rate of below 3% (1). Glioblastoma is characterised by rapid growth, high invasion, and strong resistance to radiotherapy and chemotherapy (2). Although great progress has been made with the use of modern treatments including surgery, radiotherapy, and chemotherapy, the survival time of patients with glioblastoma remains short (3). Therefore, it is necessary to identify new effective target genes to suppress the development of glioblastoma.

MicroRNAs (miRNAs/miRs) have been reported to influence the post-transcriptional reaction by binding with the 3′-UTR of the corresponding genes to result in translational suppression or degradation (4). Moreover, increasing miRNAs have been reported to control tumorigenesis, including cell growth, migration, invasion, differentiation and apoptosis (5–7). Additionally, the function of miRNAs is different in various types of cancer, such as lung adenocarcinoma, osteosarcoma and nasopharyngeal carcinoma (8–10). Some miRNAs act as tumor suppressors while others as oncogenes. Many miRNAs have been found to regulate the progression of glioblastoma. For instance, miR-137 inhibited the growth of glioblastoma through suppressing EGFR (11). miR-520c inhibited glioma cell migration and invasion by suppressing TGFBRII (12). miR-595 contributed to cell proliferation in human glioblastoma by regulating SOX7 expression (13). Among them, only a slight effect of miR-7 on glioblastoma has been identified (14,15). In addition, miR-7 with inhibitory effect has been identified in various types of cancer, such as colon cancer (16), pancreatic carcinoma (17), non-small cell lung cancer (18) and thyroid papillary cancer (19). Although the effect of miR-7 has been confirmed repeatedly, the regulatory mechanism of the miR-7/special AT rich sequence binding protein 1 (SATB1) axis has not been previously clarified.

This study focused on the change of miR-7-5p expression in glioblastoma. Moreover, the function of miR-7-5p for the migration and invasion of glioblastoma was determined. We found that miR-7-5p repressed cell mobility and invasiveness through regulation of SATB1 in glioblastoma. These observations may lead to a new approach in the treatment of glioblastoma.

Materials and methods

Clinical samples and cell culture

Forty-eight glioma specimens and adjacent tissues were taken from the Yantai Yuhuangding Hospital (Yantai, China). The patients received no treatment other than surgery, and all participants provided written informed consent. The tissue was frozen in liquid nitrogen and then stored at −80°C in a refrigerator until use. Human glioblastoma cell lines U87 MG ATCC (male malignant gliomas, ATCC HTB-14), whose origin is unknown, and U373 MG ATCC (male malignant gliomas, ATCC HTB-17) as mixed astrocytoma cells were purchased from the ATCC and cultured in DMEM supplemented with 10% fetal bovine serum (FBS). Normal human astrocytes (NHAs) were purchased from ScienCell Research Laboratories (Carlsbad, CA, USA). Although the U373 and U87 cells have been reported to be contaminated or misidentified (20–22), the use of U373 and U87 cells in this study did not affect the outcomes of this research. This experiment was approved by the Institutional Ethics Committee of Yantai Yuhuangding Hospital.

Cell transfection

The miR-7-5p mimic and inhibitor, SATB1 siRNA were obtained from GenePharma (Shanghai, China) and were transferred into U87 or U373 cells with Lipofectamine 2000 (Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturers' protocols.

RNA extraction and RT-qPCR

TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) was used to extract total RNA. RT-qPCR was carried out through the SYBR PCR Master Mix on an ABI PRISM 7900 Sequence Detection System (Applied Biosystems; Thermo Fisher Scientific, Inc.) to detect the expressions of miR-7-5p and SATB1. U6 or GAPDH served as control for miR-7-5p or SATB1. The following primers were used: miR-7 forward, 5′-AAAACTGCTGCCAAAACCAC-3′ and reverse, 5′-GCTGCATTTTACAGCGACCAA-3′; SATB1 forward, 5′-CACAGAGGTGTCTTCCGAAATCTA-3′ and reverse, 5′-AAAGCAAGCCCTGAGTTCTGTTA-3′; GAPDH forward, 5′-CGGAGTCAACGGATTTGGTCGTAT-3′ and reverse, 5′-AGCCTTCTCCATGGTGGTGAAGAC-3′; U6 forward, 5′-CTCGCTTCGGCAGCACATATACT-3′ and reverse, 5′-ACGCTTCACGAATTTGCGTGTC-3′. Amplification reaction protocol was performed for 35 cycles consisting of 94°C for 45 sec, 95°C for 15 sec, 60°C for 1 min. The miR-7-5p or SATB1 levels were analyzed using the 2−ΔΔCq method (23).

Dual luciferase assay

The wild-type (wt) 3′-UTR of SATB1 or mutant (mut) 3′-UTR of SATB1 were inserted into the pGL3 promoter vector (Sangon Biotech, Shanghai, China) for luciferase reporter experiments. The vector and miR-7-5p mimic were transfected into cells using Lipofectamine 2000 (Invitrogen; Thermo Fisher Scientific, Inc.), and 24 h after transfection, Dual-Luciferase Reporter Assay system (Beyotime Institute of Biotechnology, Beijing, China) was applied to measure luciferase activity for 30 min.

Transwell migration and invasion assay

Transwell chambers (Corning Inc., Corning, NY, USA) were applied to evaluate the migratory and invasive ability of glioblastoma cells. Transfected cells (5×104) without FBS were placed in the top chamber on the non-coated membrane, and then the lower chamber filled with 20% FBS to induce transfected cells to migrate or invade through the membrane. The cells were placed in the upper chamber with the coated membrane for the invasion assay, and were incubated for the migration and invasion assay for 48 h. The cells were then stained with crystal violet (Beyotime Institute of Biotechnology) at 37°C for 30 min. The cells (magnification, ×200) were imaged at random using an inverted microscope (Olympus Corporation, Tokyo, Japan).

Western blot analysis

Protein samples were obtained using RIPA buffer. Proteins were separated with 10% SDS-PAGE and then incubated with 5% non-fat milk blocked membranes at room temperature. Next we incubated the PVDF membranes overnight at 4°C with anti-SATB1 (dilution 1:1,000; rabbit polyclonal; cat. no. ab70004; Abcam, Cambridge, MA, USA), anti-GAPDH (dilution 1:1,000; mouse monoclonal; cat. no. 60004-1-Ig; ProteinTech, Wuhan, China) and subsequently incubated with goat anti-rabbit IgG H&L (HRP) (dilution 1:3,000; cat. no. ab6721; Abcam) secondary antibody. The 25 µl protein sample was added in the protein loaded per lane. Protein concentration was calculated using bicinchoninic acid (BCA; Beyotime Institute of Biotechnology, Shanghai, China). Then, protein expression levels were measured by ECL detecting system (Thermo Fisher Scientific, Inc.) using Bio-Rad Image-Lab software (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

Statistical analysis

Statistical analysis was performed with GraphPad Prism 6.0 (GraphPad Software, Inc., La Jolla, CA, USA) and SPSS 17.0 (SPSS, Inc., Chicago, IL, USA). Data are presented as mean ± standard deviation. Statistical analyses between two groups were performed by Student's t-test. Differences among groups were tested by one-way analysis of variance following by a Tukey's post hoc test. P<0.05 was considered to indicate a statistically significant difference.

Results

Downregulation of miR-7-5p in human glioblastoma cell lines and tissues

In order to better understand the regulation process of miR-7-5p in glioblastoma pathogenesis, we firstly identified miR-7-5p expression in glioblastoma tissues and cell lines. The observations showed that miR-7-5p expression in glioblastoma tissues was much lower than that of normal tissues (Fig. 1A). The decreased expression of miR-7-5p was also identified in U87 and U373 cell lines (Fig. 1B). All these findings indicated that abnormal expression of miR-7-5p may be related to the progression of glioblastoma.

Figure 1.

Downregulation of miR-7-5p in human glioblastoma tissues and cell lines. (A) Expression of miR-7-5p in tumor tissues and non-tumor tissues. (B) miR-7-5p expression in U87, U373 and NHAs (control). **P<0.01. NHAs, normal human astrocytes; miR, microRNA.

Cell migration and invasion of glioblastoma are inhibited by miR-7-5p

To further explore the effect of miR-7-5p in glioblastoma, miR-7-5p mimic or inhibitor was transfected into U87 cells. The efficiency of miR-7-5p expression was detected via RT-qPCR (Fig. 2A). Moreover, the Transwell assay showed that the migration and invasion in cells with miR-7-5p mimics were lower than that of miR-NC group (Fig. 2B). On the contrary, the cells with miR-7-5p inhibitor were higher than that of miR-In-NC for migration and invasion (Fig. 2C). The data revealed that miR-7-5p was a tumor suppressive miRNA for glioblastoma through inhibition of cell migration and invasion.

Figure 2.

miR-7 inhibits glioblastoma cell migration and invasion. (A) miR-7 mimic or inhibitor was transfected into U87 cells and miR-7 expression was detected via RT-qPCR. (B) Abnormal expression of miR-7 regulated migratory abilities in U87 cells (C) Ectopic expression of miR-7 regulated invasiveness in U87 cells. Magnification, ×40. **P<0.01 vs. NC. NC, negative control; miR, microRNA; miR-7, miR-7-5p.

SATB1 was the direct target of miR-7-5p in glioblastoma

Through TargetScan database (http://www.targetscan.org/vert_71/), SATB1 was identified as one of the target genes of miR-7-5p (Fig. 3A). Moreover, we performed the luciferase reporter assay to verify that miR-7-5p directly targeted SATB1. Luciferase activity in the cells containing miR-7-5p mimics and the wild-type of SATB1 was significantly reduced (P<0.01) in comparison with the control. Interestingly, there was almost no change in cells containing miR-7-5p mimic and mutant SATB1 (Fig. 3B). According to the luciferase reporter assay, we deduced that miR-7-5p directly targeted SATB1 in glioblastoma. The SATB1 expression of U87 cells containing miR-7-5p mimics or inhibitor was also detected. Apparently, the mRNA and protein expression levels of SATB1 were significantly reduced in U87 cells with miR-7-5p mimics and increased in cells containing miR-7-5p inhibitor in comparison with the control (Fig. 3C and D). In brief, miR-7-5p overexpression inhibited SATB1 expression.

Figure 3.

miR-7 directly targets SATB1 in glioblastoma cells. (A) The binding sites of wild-type of SATB1 3′-UTR combined with miR-7-5p. (B) Luciferase reporter assay. The (C) mRNA and (D) protein expression of SATB1 was analyzed in cells containing miR-7 mimic or inhibitor. **P<0.01 vs. NC. NC, negative control; miR, microRNA; SATB1, special AT rich sequence binding protein 1; miR-7, miR-7-5p.

Overexpression of SATB1 inversely reversed the suppressive effect of miR-7-5p in glioblastoma

To further confirm the observation that SATB1 promoted cell migration and invasion in glioblastoma, SATB1 siRNA was used for this investigation. Additionally, the expression of SATB1 was found to be higher in U87 and U373 cell lines than NHA cells (the control, Fig. 4A). SATB1 expression in U87 cells with SATB1 siRNA was decreased vs. the control (Fig. 4B). Migration and invasion were also impaired following SATB1 siRNA expression (Fig. 4C and D). The findings indicated that upregulation of SATB1 in glioblastoma promoted cell migration and invasion in glioblastoma.

Figure 4.

SATB1 promotes glioblastoma. (A) SATB1 expression in glioblastoma as detected by RT-qPCR. (B) SATB1 expression in cells containing si-SATB1 was confirmed by RT-qPCR. (C) Migration and (D) invasion assays in U87 cells containing si-SATB1. Magnification, ×40. **P<0.01 vs. NC. NC, negative control; miR, microRNA; SATB1, special AT rich sequence binding protein 1; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; si-, short interfering RNA.

Overexpression of SATB1 was speculated to inversely reverse the suppressive effect of miR-7-5p. To confirm that, the negative control or SATB1 expression vector was transfected into U87 cells which the miR-7-5p overexpressed. The mRNA and protein expression levels of SATB1 were decreased in cells with miR-7-5p mimic, whereas little change for SATB1 expression was found in cells with SATB1 plasmid and miR-7-5p mimic compared with the control group (Fig. 5A and B). Importantly, SATB1 overexpression was identified to weaken the inhibitory effect of miR-7-5p on cell migration and invasion in U87 cells (Fig. 5C and D). These results indicated that miR-7-5p suppressed cell migration and invasion in glioblastoma through downregulation of SATB1. The results may indicate the potential to affect tumorigenesis of glioblastoma.

Figure 5.

Overexpression of SATB1 inversely reverses the effect of miR-7. (A) Relative mRNA expression of SATB1 in the miR-7 stably-overexpressing cells. (B) The protein expression in cells containing miR-7 and SATB1. (C) Migration and (D) invasion assays were conducted in cells containing miR-7-5p mimic and SATB1. Magnification, ×40. **P<0.01 vs. NC. NC, negative control; miR, microRNA; SATB1, special AT rich sequence binding protein 1; miR-7, miR-7-5p.

Discussion

Glioblastoma was reported to develop rapidly and aggressively accounting for 15.4% of all primary brain tumors and 60–75% of astrocytoma (24). Although marked progress has been made in understanding the molecular mechanisms underlying the progression of gliomas, no targeted agent has exhibited excellent effects on patient prognosis. Therefore, it is imperative to further understand the pathogenesis of glioblastoma and to develop new targets for effective treatment.

In the present study, it was found that miR-7-5p was downregulated in glioblastoma tissues and cell lines. The overexpression of miR-7-5p inhibited cell migration and invasion of glioblastoma, whereas the downregulation of miR-7-5p had the opposite effect. Moreover, SATB1 was confirmed as a direct target of miR-7-5p. SATB1 was identified to function as an oncogene in glioblastoma. Importantly, the interaction between SATB1 and miR-7-5p was also detected. It indicated that overexpression of SATB1 could impair the suppressive function of miR-7-5p for cell motility in glioblastoma. In brief, miR-7-5p inhibited cell migration and invasion of glioblastoma through the suppression of SATB1 expression.

There is increasing evidence that miR-7 makes a contribution to the development of human cancers. Above all, Kefas et al demonstrated that miR-7 was downregulated and inhibited the EGFR and the Akt pathway in glioblastoma (25), and miR-7 interfered with the pathways of PI3K/ATK and Raf/MEK/ERK at the same time to inhibit glioblastoma growth (26). Furthermore, miR-7-5p was found to inhibit vascular endothelial cell proliferation by suppressing RAF1 and be downregulated in glioblastoma microvasculature (27). Regarding cell motility, it was reported that the migration and invasion of glioblastoma cells was inhibited by miR-15b (28), miR-154-5p (29), miR-204 (30) and miR-520c (12) through regulation of IGF1R, PIWIL1, ATF2 and TGFBRII expression. Nevertheless, whether miR-7-5p regulated the cell migration and invasion in glioblastoma was unclear. Our findings indicated that miR-7-5p acted as tumor suppressor in glioblastoma which was similar to previous findings and inhibited the cell migration and invasion as well. Furthermore, miR-7-5p was found to negatively regulate SATB1 expression by binding with its 3′-UTR.

SATB1, a global genome organizer, was reported to be upregulated in renal cell carcinoma (31), osteosarcoma (32) and bladder cancer (33). Han et al demonstrated that SATB1 was involved in the progression and prognosis of glioma (34). Chu et al proved that overexpression of SATB1 was related to the development and progression of glioma (35). Moreover, it was found that SATB1 was repressed by miR-7 in breast cancer (36). In the present study, we found a relationship between miR-7-5p and SATB1 in glioblastoma. Overexpression of SATB1 could weaken the inhibitory effect of miR-7-5p on cell migration and invasion in glioblastoma. Our observation revealed that SATB1 acts as a positive regulator for tumorigenesis of glioblastoma.

In conclusion, results of the current study demonstrated that miR-7-5p was downregulated and overexpression of miR-7-5p inhibited cell migration and invasion in glioblastoma. Moreover, miR-7-5p inhibited cell migration and invasion in glioblastoma by suppressing SATB1 expression.