| Literature DB >> 30674030 |
Michela Menegollo1, Isabella Tessari2, Luigi Bubacco3, Gyorgy Szabadkai4,5,6.
Abstract
Cytoplasmic and mitochondrial Ca2+ signals couple cellular ATP production to activity-related energy demand. In order to accurately determine the bioenergetic effect of Ca2+ signals, cellular energy charge, i.e., the compound ratio of the phosphorylated adenine nucleotides AMP, ADP, and ATP, should be estimated. Reversed-phase high-performance liquid chromatography (RP-HPLC) allows the rapid separation and quantitation of these molecules. Here we describe a protocol applied in our laboratories to quantify ATP, ADP, and AMP nucleotides in cellular extracts.Entities:
Keywords: ATP/ADP ratio; Ca2+ signal; Energy charge; Glycolysis; Oxidative phosphorylation; Reversed-phase high-performance liquid chromatography (RP-HPLC)
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Year: 2019 PMID： 30674030 DOI： 10.1007/978-1-4939-9018-4_19
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745