| Literature DB >> 30674029 |
Jennifer Wettmarshausen1,2, Fabiana Perocchi3,4.
Abstract
The development of fluorescence-based oxygen sensors coupled with microplate-based assays for quantitative bioenergetics analyses enables screening multiple experimental conditions at once with small biological material and in a timely manner. In this chapter, we outline detailed protocols and practical tips to design and perform controlled measurements of (a) respiratory and glycolytic metabolism of intact cells, (b) substrate-dependent respiration in permeabilized cells and isolated mitochondria, and (c) calcium-dependent regulation of mitochondrial bioenergetics with Seahorse XF Flux Analyzers.Entities:
Keywords: Bioenergetics; Calcium; Intact cells; Mitochondria; Oxygen consumption; Permeabilized cells; Respiration; Seahorse XF analyzer
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Year: 2019 PMID： 30674029 DOI： 10.1007/978-1-4939-9018-4_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745