Manda J Kambikambi1, Albert Chakona2, Wilbert T Kadye1. 1. Department of Ichthyology and Fisheries Science, Rhodes University, PO Box 94, Grahamstown, 6140, South Africa. 2. South African Institute for Aquatic Biodiversity, Private Bag 1015, Grahamstown, 6140, South Africa.
Abstract
RATIONALE: In trophic ecology, the use of stable isotope data relies on the general understanding of isotope turnover rates and diet-to-tissue discrimination factors (DTDFs). Recent studies on the application of stable isotope data have shown that isotope turnover rates and DTDFs can be influenced by many factors, including diet composition and tissue type. This study investigated the influence of diet composition and tissue type on stable isotope incorporation patterns in a small-bodied African minnow, the chubbyhead barb Enteromius anoplus. METHODS: The isotopic incorporation patterns of carbon (δ13 C values) and nitrogen (δ15 N values) into white muscle and caudal fin tissues of the chubbyhead barb were examined using two isotopically different diets. Controlled-diet stable isotope feeding trials using a fishmeal-based diet (diet 1) and a soya-based diet (diet 2) were conducted over a 180-day period for the chubbyhead barb. RESULTS: The two diets had contrasting isotopic incorporation patterns: diet 1 was associated with progressively high δ13 C and δ15 N values, whereas diet 2 was associated with progressively low δ13 C and δ15 N values over time for both muscle and fin tissues. The δ13 C turnover rates were similar for both tissues (56 and 61 days), whereas the δ15 N turnover rates differed between fin and muscle tissue in both diets (diet 1 = 4 and 130 days, and diet 2 = 72 and 300 days, respectively). The DTDFs were similar for both tissues in diet 1 (Δ13 C: -3.96 to -2.62‰, Δ15 N: 1.98 to 2.61‰) and diet 2 (Δ13 C: 4.05 to 5.24‰, Δ15 N: 8.45 to 9.69‰). CONCLUSIONS: These results suggest that fin tissue can potentially be used as an alternative for muscle tissue in food web studies with a reasonable level of error. The isotopic turnover rate and DTDFs estimates for E. anoplus, however, require consideration of diet composition because different diets may differ in their isotopic incorporation patterns.
RATIONALE: In trophic ecology, the use of stable isotope data relies on the general understanding of isotope turnover rates and diet-to-tissue discrimination factors (DTDFs). Recent studies on the application of stable isotope data have shown that isotope turnover rates and DTDFs can be influenced by many factors, including diet composition and tissue type. This study investigated the influence of diet composition and tissue type on stable isotope incorporation patterns in a small-bodied African minnow, the chubbyhead barbEnteromius anoplus. METHODS: The isotopic incorporation patterns of carbon (δ13 C values) and nitrogen (δ15 N values) into white muscle and caudal fin tissues of the chubbyhead barb were examined using two isotopically different diets. Controlled-diet stable isotope feeding trials using a fishmeal-based diet (diet 1) and a soya-based diet (diet 2) were conducted over a 180-day period for the chubbyhead barb. RESULTS: The two diets had contrasting isotopic incorporation patterns: diet 1 was associated with progressively high δ13 C and δ15 N values, whereas diet 2 was associated with progressively low δ13 C and δ15 N values over time for both muscle and fin tissues. The δ13 C turnover rates were similar for both tissues (56 and 61 days), whereas the δ15 N turnover rates differed between fin and muscle tissue in both diets (diet 1 = 4 and 130 days, and diet 2 = 72 and 300 days, respectively). The DTDFs were similar for both tissues in diet 1 (Δ13 C: -3.96 to -2.62‰, Δ15 N: 1.98 to 2.61‰) and diet 2 (Δ13 C: 4.05 to 5.24‰, Δ15 N: 8.45 to 9.69‰). CONCLUSIONS: These results suggest that fin tissue can potentially be used as an alternative for muscle tissue in food web studies with a reasonable level of error. The isotopic turnover rate and DTDFs estimates for E. anoplus, however, require consideration of diet composition because different diets may differ in their isotopic incorporation patterns.
Authors: Francesca Tulli; José M Moreno-Rojas; Concetta Maria Messina; Angela Trocino; Gerolamo Xiccato; José M Muñoz-Redondo; Andrea Santulli; Emilio Tibaldi Journal: Animals (Basel) Date: 2020-11-05 Impact factor: 2.752