Caroline Klingebiel1, Yannick Chantran2,3, Rihane Arif-Lusson4, Angelica E Ehrenberg5, Jonas Östling5, Alain Poisson6, Valérie Liabeuf7, Chantal Agabriel8, Joëlle Birnbaum9, Françoise Porri6, Anne Sarrat10, Pol-André Apoil11, Mylène Vivinus12, Lorna Garnier13, Anca Mirela Chiriac14,15, Davide-Paolo Caimmi14,15, Jean-Luc Bourrain14, Pascal Demoly14,15, Stéphane Guez16, Franck Boralevi17, Bertrand Lovato18, Céline Palussière19, Sylvie Leroy20, Thierry Bourrier21, Lisa Giovannini-Chami21, Marion Gouitaa22, Agnès Aferiat-Derome23, Denis Charpin24, Tünde Sofalvi22, Isabelle Cabon-Boudard25, Yann-Patrick Massabie-Bouchat26, Bernard Hofmann27, Nathalie Bonardel28, Mireille Dron-Gonzalvez29, Benoît Sterling26,30, Ania Carsin30, Serge Vivinus20, Bernard Poitevin31, Laureline Nicolau32, Geneviève Liautard33, Christophe Soler1, Soraya Mezouar4, Isabella Annesi-Maesano15, Jean-Louis Mège4, Jonas Lidholm5, Joana Vitte4. 1. Laboratoire Synlab Provence, Marseille, France. 2. UPMC Univ Paris 06, INSERM UMRS 938, Centre de Recherche Saint-Antoine, team "Immune System, Neuroinflammation and Neurodegenerative Diseases", Hôpital Saint-Antoine, Sorbonne Universités, Paris, France. 3. Immunology Department, AP-HP Saint-Antoine Hospital, Paris, France. 4. Aix-Marseille Univ, IRD, APHM, MEPHI, IHU Méditerranée Infection, Marseille, France. 5. Thermo Fisher Scientific, Uppsala, Sweden. 6. Service de Pneumo-Allergologie, Hôpital Saint Joseph, Marseille, France. 7. Aix-Marseille Univ, APHM, Hôpital Timone, Service de Dermatologie-Vénéréologie, Marseille, France. 8. Aix-Marseille Univ, APHM, Hôpital Timone, Service de Pédiatrie Multidisciplinaire, Marseille, France. 9. Service de Pneumologie et Allergologie, CH du Pays d'Aix, Aix-en-Provence, France. 10. Laboratoire d'Immunologie et Immunogénétique, GH Pellegrin, CHU Bordeaux, Bordeaux, France. 11. Institut Fédératif de Biologie, Hôpital Purpan, CHU Toulouse, Toulouse, France. 12. Laboratoire d'Immunologie, Hôpital de l'Archet, CHU Nice, Nice, France. 13. Laboratoire d'Immunologie, CH Lyon Sud, CHU Lyon, Pierre-Bénite, France. 14. Département de pneumologie et addictologie, CHU Montpellier, Hôpital Arnaud-de-Villeneuve, Univ Montpellier, Montpellier, France. 15. Sorbonne Universités, INSERM UMRS 1136, IPLESP, team EPAR, Paris, France. 16. Unité d'allergologie, GH Pellegrin, CHU Bordeaux, Bordeaux, France. 17. Unité de Dermatologie Pédiatrique, Hôpital Pellegrin-Enfants, CHU Bordeaux, Bordeaux, France. 18. Medical Office, Mérignac, France. 19. Medical Office, Cenon, France. 20. Service de Pneumologie, Hôpital Pasteur, CHU Nice, Nice, France. 21. Hôpitaux Pédiatriques de Nice, CHU Lenval, Nice, France. 22. Aix-Marseille Univ, APHM, Hôpital Nord, Service de Pneumologie, Marseille, France. 23. Medical Office, Les Jardins de Castellane, Marseille, France. 24. Aix-Marseille Univ, APHM, Hôpital Timone, Unité de Pneumologie, Marseille, France. 25. Aix-Marseille Univ, APHM, Hôpital Timone, Service de Chirurgie Pédiatrique, Marseille, France. 26. Medical Office, Marseille, France. 27. Medical Office, Carpentras, France. 28. Médicale Victor Hugo, Avignon, France. 29. Medical Office, Martigues, France. 30. Aix-Marseille Univ, APHM, Hôpital Timone, Service de Pneumo-Pédiatrie, Marseille, France. 31. Medical Office, Bormes-les-Mimosas, France. 32. Medical Office, Perpignan, France. 33. Medical Office, Solliès-Pont, France.
Abstract
BACKGROUND: Peach is a common elicitor of food allergic reactions. Peach-induced immediate reactions may occur as benign pollen-food syndromes, usually due to birch pollen-related PR-10 cross-reactivity in temperate climates, and as potentially severe primary food allergies, predominantly related to nsLTP Pru p 3 in Mediterranean regions. The newly described peach allergen Pru p 7 has gained recent attention as a potential peach allergy severity marker. Sensitization to Pru p 7 and its allergenic homologues of the gibberellin-regulated protein family occurs in areas with high Cupressaceae tree pollen exposure. OBJECTIVE: We sought to investigate the distribution, clinical characteristics and molecular associations of Pru p 7 sensitization among subjects with suspected peach allergy in different regions of France. METHODS: Subjects with suspected peach allergy (n = 316) were included. Diagnostic work-up was performed according to current guidelines, including open food challenge when required. IgE antibody measurements and competition experiments were performed using the ImmunoCAP assay platform. RESULTS: Sensitization to Pru p 7 was present in 171 (54%) of all subjects in the study and in 123 of 198 (62%) diagnosed as peach allergic, more than half of whom were sensitized to no other peach allergen. Frequency and magnitude of Pru p 7 sensitization were associated with the presence of peach allergy, the clinical severity of peach-induced allergic reactions and the level of cypress pollen exposure. Cypress pollen extract completely outcompeted IgE binding to Pru p 7. Pru p 7 was extremely potent in basophil activation tests. CONCLUSION AND CLINICAL RELEVANCE: A subtype of Cupressaceae pollinosis, characterized by Pru p 7 sensitization, can be an underlying cause of severe peach allergy.
BACKGROUND:Peach is a common elicitor of food allergic reactions. Peach-induced immediate reactions may occur as benign pollen-food syndromes, usually due to birch pollen-related PR-10 cross-reactivity in temperate climates, and as potentially severe primary food allergies, predominantly related to nsLTP Pru p 3 in Mediterranean regions. The newly described peach allergen Pru p 7 has gained recent attention as a potential peach allergy severity marker. Sensitization to Pru p 7 and its allergenic homologues of the gibberellin-regulated protein family occurs in areas with high Cupressaceae tree pollen exposure. OBJECTIVE: We sought to investigate the distribution, clinical characteristics and molecular associations of Pru p 7 sensitization among subjects with suspected peach allergy in different regions of France. METHODS: Subjects with suspected peach allergy (n = 316) were included. Diagnostic work-up was performed according to current guidelines, including open food challenge when required. IgE antibody measurements and competition experiments were performed using the ImmunoCAP assay platform. RESULTS: Sensitization to Pru p 7 was present in 171 (54%) of all subjects in the study and in 123 of 198 (62%) diagnosed as peach allergic, more than half of whom were sensitized to no other peach allergen. Frequency and magnitude of Pru p 7 sensitization were associated with the presence of peach allergy, the clinical severity of peach-induced allergic reactions and the level of cypress pollen exposure. Cypress pollen extract completely outcompeted IgE binding to Pru p 7. Pru p 7 was extremely potent in basophil activation tests. CONCLUSION AND CLINICAL RELEVANCE: A subtype of Cupressaceae pollinosis, characterized by Pru p 7 sensitization, can be an underlying cause of severe peach allergy.
Authors: Juan Carlos Vizuet-de-Rueda; Josaphat Miguel Montero-Vargas; Miguel Ángel Galván-Morales; Raúl Porras-Gutiérrez-de-Velasco; Luis M Teran Journal: Int J Mol Sci Date: 2022-05-20 Impact factor: 6.208