Soumaya Hajaji1,2, Mohamed-Amine Jabri3, Dhouha Alimi4,5, Mourad Rekik6, Hafidh Akkari4. 1. Laboratoire de Parasitologie, Université de la Manouba, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020, Sidi Thabet, Tunisia. soumayahajjaji86@gmail.com. 2. Faculté des Sciences de Bizerte, Université de Carthage, 7021, Zarzouna, Tunisia. soumayahajjaji86@gmail.com. 3. Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources, Institut Supérieur de Biotechnologie de Béja, Université de Jendouba, Avenue Habib Bourguiba, B.P. 382, 9000, Béja, Tunisia. 4. Laboratoire de Parasitologie, Université de la Manouba, École Nationale de Médecine Vétérinaire de Sidi Thabet, 2020, Sidi Thabet, Tunisia. 5. Faculté des Sciences de Bizerte, Université de Carthage, 7021, Zarzouna, Tunisia. 6. International Center for Agricultural Research in the Dry Areas (ICARDA), P.O. Box 950764, Amman, 11195, Jordan.
Abstract
BACKGROUND: Chamomile (Matricaria recutita L.) is a plant which has been reported to be effective in treating several parasitic and digestive diseases. The present study was conducted to evaluate the anthelmintic activity of chamomile methanolic extract (CME). METHODS: In vitro, the anthelmintic activities of CME were investigated on the L3 larvae of Heligmosomoides polygyrus in comparison to albendazole. In vivo, Swiss albino mice were infected with infective third (L3) larval stage of H. polygyrus by intragastric administration. Moreover, the effect of CME and albendazole on worm eggs, adult worms, serum cytokine production, and oxidative stress was studied. RESULTS: All used doses of CME showed a potent anthelmintic activity both in vitro and in vivo and the effect being similar to treatment with albendazole. Moreover, H. polygyrus infestation was accompanied by an intestinal oxidative stress status characterized by an increased lipoperoxidation, a depletion of antioxidant enzyme activity, as well as an overload of hydrogen peroxide. We have also recorded an increase of pro-inflammatory mediator (TNF-α, IL-6, and IL-1β) levels after treatment with CME (14 ± 0.8; 41 ± 2; 58 ± 4 pg/mg protein, respectively, with the concentration 800 mg/kg, body weight) when compared with infected control mice (20 ± 1; 59 ± 2, and 83 ± 4 pg/mg protein, respectively). However, extract treatment alleviated all the deleterious effects associated with H. polygyrus infection. CONCLUSION: These findings suggest that CME can be used in the control of gastrointestinal helminthiasis and associated oxidative stress.
BACKGROUND: Chamomile (Matricaria recutita L.) is a plant which has been reported to be effective in treating several parasitic and digestive diseases. The present study was conducted to evaluate the anthelmintic activity of chamomile methanolic extract (CME). METHODS: In vitro, the anthelmintic activities of CME were investigated on the L3 larvae of Heligmosomoides polygyrus in comparison to albendazole. In vivo, Swiss albino mice were infected with infective third (L3) larval stage of H. polygyrus by intragastric administration. Moreover, the effect of CME and albendazole on worm eggs, adult worms, serum cytokine production, and oxidative stress was studied. RESULTS: All used doses of CME showed a potent anthelmintic activity both in vitro and in vivo and the effect being similar to treatment with albendazole. Moreover, H. polygyrus infestation was accompanied by an intestinal oxidative stress status characterized by an increased lipoperoxidation, a depletion of antioxidant enzyme activity, as well as an overload of hydrogen peroxide. We have also recorded an increase of pro-inflammatory mediator (TNF-α, IL-6, and IL-1β) levels after treatment with CME (14 ± 0.8; 41 ± 2; 58 ± 4 pg/mg protein, respectively, with the concentration 800 mg/kg, body weight) when compared with infected control mice (20 ± 1; 59 ± 2, and 83 ± 4 pg/mg protein, respectively). However, extract treatment alleviated all the deleterious effects associated with H. polygyrus infection. CONCLUSION: These findings suggest that CME can be used in the control of gastrointestinal helminthiasis and associated oxidative stress.
Authors: Felipe Galeti Miguel; Amanda Henriques Cavalheiro; Nathália Favaretto Spinola; Diego Luis Ribeiro; Gustavo Rafael Mazzaron Barcelos; Lusânia Maria Greggi Antunes; Juliana Issa Hori; Franciane Marquele-Oliveira; Bruno Alves Rocha; Andresa Aparecida Berretta Journal: Evid Based Complement Alternat Med Date: 2015-09-03 Impact factor: 2.629