Evaluation of cytotoxicity, hemocompatibility and spectral studies of chitosan assisted polyurethanes prepared with various diisocyanates.

In this research work cytocompatibility, mutagenicity, and hemolytic activity of chitosan-based polyurethanes (PUs) have been evaluated. The chitosan modified PUs were prepared by step-growth polymerization technique using various diisocyanates like isophorone diisocyanate (IPDI). 4,4'-methylenedicyclohexyl diisocyanate (H12MDI), 2,4-toluene diisocyanate (TDI) and hexamethylene diisocyanate (HMDI) by reacting with hydroxyl-terminated polybutadiene (HTPB). Structural confirmation of prepared samples was done by FTIR-ATR and 1H NMR techniques. Chitosan bearing PU samples showed good hemocompatibility, non-mutagenic behavior and less or non-cytotoxic behavior with all the diisocyanates. Among all the diisocyanates, aromatic diisocyanate (TDI) showed less hemocompatibility, high mutagenicity, and more cytotoxicity. However, this still showed a better result than non-chitosan based sample. It is concluded that chitosan improved the biological behavior of PU samples.