Thi Tho Bui1, Da-Ae Kwon2, Dae Woon Choi3, Sun Young Jung3, So-Young Lee4, Chun Hua Piao5, Eunjin Hyeon5, Yanjing Fan5, Sung Hum Yeon6, Rak-Ho Son6, Dong-Hwa Shon2, Chang Ho Song7, Hee Soon Shin8, Ok Hee Chai9. 1. Department of Anatomy, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea; Faculty of Biology & Environmental Science, University of Education, The University of Danang, Danang 555940, Vietnam. 2. Research Division of Food Functionality, Korea Food Research Institute, Wanju-gun, 55365, Republic of Korea. 3. Food Biotechnology Program, Korea University of Science and Technology, Daejeon 305-350, Republic of Korea. 4. Research Division of Food Functionality, Korea Food Research Institute, Wanju-gun, 55365, Republic of Korea.; Food Biotechnology Program, Korea University of Science and Technology, Daejeon 305-350, Republic of Korea. 5. Department of Anatomy, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea. 6. R&D Center, Huons. Co. Ltd., College of Pharmacy, Hanyang University, Sangnok-gu, Ansan-si, Kyeonggi-do 15588, Republic of Korea. 7. Department of Anatomy, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea; Institute for Medical Sciences, Chonbuk National University, Jeonju, Jeonbuk 54896, Republic of Korea. 8. Research Division of Food Functionality, Korea Food Research Institute, Wanju-gun, 55365, Republic of Korea.; Food Biotechnology Program, Korea University of Science and Technology, Daejeon 305-350, Republic of Korea.. Electronic address: hsshin@kfri.re.kr. 9. Department of Anatomy, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea; Institute for Medical Sciences, Chonbuk National University, Jeonju, Jeonbuk 54896, Republic of Korea.. Electronic address: okchai1004@jbnu.ac.kr.
Abstract
BACKGROUND: Rosae Multiflorae fructus has potent antioxidative, analgesic, and anti-inflammatory properties. PURPOSE: We investigated the immunomodulatory effect of Rosae Multiflorae fructus extract (RMFE) on allergic inflammation in an allergic rhinitis (AR) mouse model. METHODS: Mice were sensitized and intranasally challenged with ovalbumin (OVA), the Th1/Th2-related cytokines and histopathology were examinated after RMFE treatments. Primary cell culture from spleen and NALT was performed to evaluate RMFE effect on Th1/Th2 responses. Four active components of RMFE were determined using HPLC and then tested the inhibition on Th2 response. RESULTS: Oral administration of RMFE inhibited the accumulation of eosinophils in nasal lavage fluid (NALF) and the nasal mucosa, goblet cells in the nasal epithelium, and mast cells in the respiratory region of the nasal cavity. Thus, the swelling of the nasal epithelium, nasal-associated lymphoid tissue (NALT), and lung tissue were ameliorated. Furthermore, the RMFE suppressed Th2-related cytokines, such as IL-4, IL-5, and IL-13 in NALF, NALT, and splenocytes, whereas the Th1-associated cytokine IL-12 was up-regulated by RMFE. We also revealed the active components of RMFE, such as ellagic acid, hyperoside, isoquercitrin, and miquelianin. They may inhibit IL-4 secretion in allergic responses. CONCLUSION: RMFE may have therapeutic potential for treating AR by modulating the relationships between Th1/Th2 responses.
BACKGROUND: Rosae Multiflorae fructus has potent antioxidative, analgesic, and anti-inflammatory properties. PURPOSE: We investigated the immunomodulatory effect of Rosae Multiflorae fructus extract (RMFE) on allergic inflammation in an allergic rhinitis (AR) mouse model. METHODS:Mice were sensitized and intranasally challenged with ovalbumin (OVA), the Th1/Th2-related cytokines and histopathology were examinated after RMFE treatments. Primary cell culture from spleen and NALT was performed to evaluate RMFE effect on Th1/Th2 responses. Four active components of RMFE were determined using HPLC and then tested the inhibition on Th2 response. RESULTS: Oral administration of RMFE inhibited the accumulation of eosinophils in nasal lavage fluid (NALF) and the nasal mucosa, goblet cells in the nasal epithelium, and mast cells in the respiratory region of the nasal cavity. Thus, the swelling of the nasal epithelium, nasal-associated lymphoid tissue (NALT), and lung tissue were ameliorated. Furthermore, the RMFE suppressed Th2-related cytokines, such as IL-4, IL-5, and IL-13 in NALF, NALT, and splenocytes, whereas the Th1-associated cytokine IL-12 was up-regulated by RMFE. We also revealed the active components of RMFE, such as ellagic acid, hyperoside, isoquercitrin, and miquelianin. They may inhibit IL-4 secretion in allergic responses. CONCLUSION:RMFE may have therapeutic potential for treating AR by modulating the relationships between Th1/Th2 responses.