Emanuele Calabrò1,2, Salvatore Magazù1,3,4,5,6. 1. a Department of Mathematical and Informatics Sciences , Physical Sciences and Earth Sciences, University of Messina , Messina , Italy. 2. b CISFA, Interuniversity Consortium of Applied Physical Sciences , Messina , Italy. 3. c Le Studium, Loire Valley Institute for Advanced Studies , Orléans , France. 4. d Centre de Biophysique Moleculaire (CBM), CNRS UPR 4301 du CNRS , Orleans CEDEX 2 , France. 5. e Laboratoire Interfaces, Confinement, Matériaux et Nanostructures (ICMN), UMR 7374 CNRS, Université d'Orléans , Orléans cedex 2 , France. 6. f Istituto Nazionale di Alta Matematica "F. Severi" - INDAM, Gruppo Nazionale per la Fisica Matematica - GNFM, Rome, Italy.
Abstract
Purpose: To study the response of neuronal-like cells to an applied static or low-frequency magnetic field. Materials and methods: Fourier Transform Infrared (FTIR) Spectroscopy was used to investigate the overall behavior of SH-SY5Y neuronal-like cells exposed to a static or 50 Hz magnetic fields at intensities up to 1 mT generated by two Helmholtz coils driven by direct current or alternate current voltage. The Helmholtz coils were placed into an incubator in a 5% CO2/95% air humidified at 37 °C. Results: A significant increase in intensity of the Amide I band and of CH2 stretching vibrations in neuronal-like FTIR spectra appeared after 6 h exposure to static or 50 Hz magnetic fields. Conclusions: These findings can be easily explained assuming that proteins α-helices and lipids contents in cellular membrane aligned toward the direction of the applied magnetic field after exposure, inducing an increase of ions flux across cellular membrane channels after exposure to a magnetic field, changing cellular functions.
Purpose: To study the response of neuronal-like cells to an applied static or low-frequency magnetic field. Materials and methods: Fourier Transform Infrared (FTIR) Spectroscopy was used to investigate the overall behavior of SH-SY5Y neuronal-like cells exposed to a static or 50 Hz magnetic fields at intensities up to 1 mT generated by two Helmholtz coils driven by direct current or alternate current voltage. The Helmholtz coils were placed into an incubator in a 5% CO2/95% air humidified at 37 °C. Results: A significant increase in intensity of the Amide I band and of CH2 stretching vibrations in neuronal-like FTIR spectra appeared after 6 h exposure to static or 50 Hz magnetic fields. Conclusions: These findings can be easily explained assuming that proteins α-helices and lipids contents in cellular membrane aligned toward the direction of the applied magnetic field after exposure, inducing an increase of ions flux across cellular membrane channels after exposure to a magnetic field, changing cellular functions.
Entities:
Keywords:
50 Hz magnetic field; Amide I; CH vibration; FTIR Spectroscopy; cellular membrane channel; neuronal-like cells; static magnetic field
Authors: Palalle G Tharushi Perera; Denver P Linklater; Erim Kosyer; Rodney Croft; Elena P Ivanova Journal: R Soc Open Sci Date: 2022-06-29 Impact factor: 3.653