Manuela Costanzo1, Federica Vurro1, Barbara Cisterna1, Federico Boschi2, Alessandro Marengo3, Elita Montanari4, Chiara Di Meo4, Pietro Matricardi4, Gloria Berlier5, Barbara Stella3, Silvia Arpicco3, Manuela Malatesta1. 1. Department of Neurosciences, Biomedicine & Movement Sciences, University of Verona, Strada Le Grazie, 8 - 37134 Verona, Italy. 2. Department of Computer Science, University of Verona, Strada Le Grazie, 15 - 37134 Verona, Italy. 3. Department of Drug Science & Technology, University of Turin, Via P. Giuria, 9 - 10125 Torino, Italy. 4. Department of Drug Chemistry & Technologies, Sapienza University of Rome, Piazzale Aldo Moro, 5 - 00185 Roma, Italy. 5. Department of Chemistry & NIS Centre, University of Turin, Via P. Giuria, 7 - 10125 Torino, Italy.
Abstract
AIM: To elucidate whether different cytokinetic features (i.e., presence or absence of mitotic activity) may influence cell uptake and distribution of nanocarriers, in vitro tests on liposomes, mesoporous silica nanoparticles, poly(lactide-co-glycolide) nanoparticles and nanohydrogels were carried out on C2C12 murine muscle cells either able to proliferate as myoblasts (cycling cells) or terminally differentiate into myotubes (noncycling cells). MATERIALS & METHODS: Cell uptake and intracellular fate of liposomes, mesoporous silica nanoparticles, poly(lactide-co-glycolide) nanoparticles and nanohydrogels were investigated by confocal fluorescence microscopy and transmission electron microscopy. RESULTS: Nanocarrier internalization and distribution were similar in myoblasts and myotubes; however, myotubes demonstrated a lower uptake capability. CONCLUSION: All nanocarriers proved to be suitably biocompatible for both myoblasts and myotubes. The lower uptake capability of myotubes is probably due to different plasma membrane composition related to the differentiation process.
AIM: To elucidate whether different cytokinetic features (i.e., presence or absence of mitotic activity) may influence cell uptake and distribution of nanocarriers, in vitro tests on liposomes, mesoporous silica nanoparticles, poly(lactide-co-glycolide) nanoparticles and nanohydrogels were carried out on C2C12 murine muscle cells either able to proliferate as myoblasts (cycling cells) or terminally differentiate into myotubes (noncycling cells). MATERIALS & METHODS: Cell uptake and intracellular fate of liposomes, mesoporous silica nanoparticles, poly(lactide-co-glycolide) nanoparticles and nanohydrogels were investigated by confocal fluorescence microscopy and transmission electron microscopy. RESULTS: Nanocarrier internalization and distribution were similar in myoblasts and myotubes; however, myotubes demonstrated a lower uptake capability. CONCLUSION: All nanocarriers proved to be suitably biocompatible for both myoblasts and myotubes. The lower uptake capability of myotubes is probably due to different plasma membrane composition related to the differentiation process.