| Literature DB >> 3066700 |
A M Berry1, J C Paton, E M Glare, D Hansman, D E Catcheside.
Abstract
A gene bank of Sau3AI-generated Streptococcus pneumoniae DNA fragments was constructed in Escherichia coli K-12 by cloning into the BamHI site of the cosmid vector pHC79. One clone capable of cleaving the fluorogenic neuraminidase substrate 2'-(4-methylumbelliferyl)-alpha-D-N-acetyl-neuraminic acid was isolated. This activity was inhibited by treatment with a mouse antiserum raised against purified pneumococcal neuraminidase. The recombinant plasmid purified from this clone (designated pJCP301) contained approximately 3.0 kb of pneumococcal DNA. Western-blot analysis indicated that E. coli K-12[pJCP301] produced a 98-kDa polypeptide which reacted with antineuraminidase serum.Entities:
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Year: 1988 PMID： 3066700 DOI： 10.1016/0378-1119(88)90046-7
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688