| Literature DB >> 30666038 |
Acharaporn Duangjai1, Nitra Nuengchamnong2, Nungruthai Suphrom3, Kanittaporn Trisat4, Nanteetip Limpeanchob4, Surasak Saokaew5,6,7,8.
Abstract
This study was to assess the impact of different colors of coffee fruit (green, yellow and red) on adipogenesis and/or lipolysis using 3T3-L1 adipocytes. Characterization of chemical constituents in different colors of coffee fruit extracts was determined by ESI-Q-TOF-MS. The cytotoxicity of the extracts in 3T3-L1 preadipocytes were evaluated by MTT assay. Oil-red O staining and amount of glycerol released in 3T3-L1 adipocytes were measured for lipid accumulation and lipolysis activity. All coffee fruit extracts displayed similar chromatographic profiles by chlorogenic acid > caffeoylquinic acid > caffeic acid. Different colors of raw coffee fruit possessed inhibitory adipogenesis activity in 3T3-L1 adipocytes, especially CRD decreased lipid accumulation approximately 47%. Furthermore, all extracts except CYF and their major compounds (malic, quinic, and chlorogenic acid) increased glycerol release. Our data suggest that different colors of coffee fruit extract have possessed anti-adipogenic and lipolytic properties and may contribute to the anti-obesity effects.Entities:
Keywords: adipogenesis; chlorogenic acid; coffee; lipolysis; quinic acid
Mesh：
Substances：
Year: 2018 PMID： 30666038 PMCID： PMC6347046
Source DB: PubMed Journal: Kobe J Med Sci ISSN： 0023-2513