Toon Peeters1,2,3,4, John Penders5, Sanne P Smeekens3,4, Gianluca Galazzo5, Bert Houben6, Mihai G Netea3,4, Paul Hm Savelkoul5,7, Inge C Gyssens1,2,3,4. 1. Department of Infectious Diseases & Immunity, Jessa Hospital, Hasselt, Belgium. 2. Faculty of Medicine & Life Sciences, Hasselt University, Hasselt, Belgium. 3. Department of Internal Medicine, Radboud University Medical Center, Nijmegen, The Netherlands. 4. Radboudumc Center for Infectious Diseases (RCI), Radboud University Medical Center, Nijmegen, The Netherlands. 5. School of Nutrition & Translational Research in Metabolism (NUTRIM) & School for Public Health & Primary Care (CAPHRI), Department of Medical Microbiology, Maastricht University Medical Center+, Maastricht, The Netherlands. 6. Department of Abdominal & Oncological Surgery, Jessa Hospital, Hasselt, Belgium. 7. Department of Medical Microbiology & Infection Control, VU University Medical Center, Amsterdam, The Netherlands.
Abstract
AIM: We aimed to study the mucosal microbiota of the appendix in a prospective appendicitis cohort and to compare the fecal microbiota of patients and controls. We hypothesized that the microbiota may be associated with susceptibility to appendicitis. PATIENTS & METHODS: The fecal microbiota of 99 patients and 106 controls were characterized using 16S-23S intergenic spacer profiling. Richness, diversity and community structure were compared. The appendiceal microbiota from 90 patients was analyzed according to the severity of appendicitis. RESULTS: Overall fecal microbial richness and diversity were similar in patients and controls, yet richness and diversity within the group of Firmicutes, Actinobacteria, Fusobacteria and Verrucomicrobia phyla were lower in patients. Discriminant analyses could correctly classify patients and controls with fair accuracy. No differences were found according to severity in appendiceal or fecal microbiota. CONCLUSION: This study demonstrates differences in the composition of intestinal microbiota of appendicitis patients and healthy individuals.
AIM: We aimed to study the mucosal microbiota of the appendix in a prospective appendicitis cohort and to compare the fecal microbiota of patients and controls. We hypothesized that the microbiota may be associated with susceptibility to appendicitis. PATIENTS & METHODS: The fecal microbiota of 99 patients and 106 controls were characterized using 16S-23S intergenic spacer profiling. Richness, diversity and community structure were compared. The appendiceal microbiota from 90 patients was analyzed according to the severity of appendicitis. RESULTS: Overall fecal microbial richness and diversity were similar in patients and controls, yet richness and diversity within the group of Firmicutes, Actinobacteria, Fusobacteria and Verrucomicrobia phyla were lower in patients. Discriminant analyses could correctly classify patients and controls with fair accuracy. No differences were found according to severity in appendiceal or fecal microbiota. CONCLUSION: This study demonstrates differences in the composition of intestinal microbiota of appendicitispatients and healthy individuals.
Authors: Sun Jung Oh; Maya Pimentel; Gabriela G S Leite; Shreya Celly; Maria Jesus Villanueva-Millan; Isabela Lacsina; Brennan Chuang; Gonzalo Parodi; Walter Morales; Stacy Weitsman; Tahli Singer-Englar; Gillian M Barlow; Jing Zhai; Nipaporn Pichestshote; Ali Rezaie; Ruchi Mathur; Mark Pimentel Journal: BMJ Open Gastroenterol Date: 2020-06
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Authors: Toon Peeters; Sandrina Martens; Valentino D'Onofrio; Mark H T Stappers; Jeroen C H van der Hilst; Bert Houben; Ruth Achten; Leo A B Joosten; Inge C Gyssens Journal: Sci Rep Date: 2020-10-15 Impact factor: 4.379
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