Hui Yang1, Li-Tao Yang2,3, Jun Liu4, Shan Tang1, Xiulan Zhao1, Qiaohong Wang1, Shaoguo Zhang1, Meixia Shi1, Wei Pan1, Ping-Chang Yang2. 1. Department of Nursing, The First Hospital, Shanxi Medical University Taiyuan 030001, Shanxi, China. 2. The Research Center of Allergy & Immunology, School of Medicine, Shenzhen University Shenzhen 518060, Shanxi, China. 3. Longgang ENT Hospital Shenzhen 518116, Shanxi, China. 4. Shenzhen Maternity & Child Health Hospital Shenzhen 518052, Shanxi, China.
Abstract
BACKGROUND AND AIMS: The mechanism of dysfunction of regulatory B cells is unclear. The circadian locomotor output cycles kaput (CLK) regulates immune responses. CLK expression can be increased by alteration of the circadian rhythm. This study tests a hypothesis that alteration of the circadian rhythm, such as engaging the day-night shift rotation (DNSR), interferes with the expression of transforming growth factor (TGF)-β in B cells (TGFbB cell). METHODS: Peripheral blood samples were collected from DNSR nurses and persons with the regular circadian clock life style (RC). The frequency of TGFbB cells in the blood samples was assessed by flow cytometry. The expression of TGF-beta in B cells was assessed with real time RT-PCR. RESULTS: We observed that the frequency of peripheral TGFbB cells was less in DNSR nurses as compared to RC subjects. The expression of CLK and histone deacetylase 11 in peripheral B cells was higher, the TGF-β expression was lower, in peripheral B cells of DNSR nurses. Over-expression of CLK repressed the expression of TGF-β in B cells, which was mediated by HDAC11. CONCLUSIONS: The CLK expression in peripheral B cells is higher in DNSR nurses, which suppresses the expression of TGF-β in B cells. To regulate the expression of CLK during the circadian clock alteration needs to be further investigated.
BACKGROUND AND AIMS: The mechanism of dysfunction of regulatory B cells is unclear. The circadian locomotor output cycles kaput (CLK) regulates immune responses. CLK expression can be increased by alteration of the circadian rhythm. This study tests a hypothesis that alteration of the circadian rhythm, such as engaging the day-night shift rotation (DNSR), interferes with the expression of transforming growth factor (TGF)-β in B cells (TGFbB cell). METHODS: Peripheral blood samples were collected from DNSR nurses and persons with the regular circadian clock life style (RC). The frequency of TGFbB cells in the blood samples was assessed by flow cytometry. The expression of TGF-beta in B cells was assessed with real time RT-PCR. RESULTS: We observed that the frequency of peripheral TGFbB cells was less in DNSR nurses as compared to RC subjects. The expression of CLK and histone deacetylase 11 in peripheral B cells was higher, the TGF-β expression was lower, in peripheral B cells of DNSR nurses. Over-expression of CLK repressed the expression of TGF-β in B cells, which was mediated by HDAC11. CONCLUSIONS: The CLK expression in peripheral B cells is higher in DNSR nurses, which suppresses the expression of TGF-β in B cells. To regulate the expression of CLK during the circadian clock alteration needs to be further investigated.
Entities:
Keywords:
B lymphocyte; circadian CLK; interleukin-10; nuclear factor-interleukin-3; nurse
Authors: Luca Coppeta; Cristiana Ferrari; Marco Trabucco Aurilio; Gianluigi Ferrazza; Andrea Magrini; Stefano Rizza Journal: Int J Environ Res Public Health Date: 2022-07-20 Impact factor: 4.614