Carlos Palomares-Reyes1, Wilmer Silva-Caso2, Luis J Del Valle3, Miguel Angel Aguilar-Luis4, Claudia Weilg1, Johanna Martins-Luna1, Adriana Viñas-Ospino1, Luciana Stimmler5, Naysha Mallqui Espinoza6, Ronald Aquino Ortega1, Walter Espinoza Espíritu7, Erika Misaico8, Juana Del Valle-Mendoza9. 1. School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru. 2. School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru; Centro de Salud las Palmas, Red de Salud Leoncio Prado, Tingo María, Peru. 3. Barcelona Research Center for Multiscale Science and Engineering, Departament d'Enginyeria Quıímica EEBE, Universidad Politècnica de Catalunya (UPC), Barcelona, Spain. 4. School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru; Laboratorio de Biología Molecular, Instituto de Investigación Nutricional, Lima, Peru; Instituto de Investigación de Enfermedades Infecciosas, Lima, Peru. 5. Laboratorio de Biología Molecular, Instituto de Investigación Nutricional, Lima, Peru. 6. Universidad Nacional Agraria de la Selva Tingo María, Huánuco, Peru. 7. Puesto de Salud Tambillo Grande, Red de Salud Leoncio Prado, Huánuco, Peru. 8. Hospital de Tingo María, Ministerio de Salud del Peru, Huánuco, Peru. 9. School of Medicine, Research and Innovation Centre of the Faculty of Health Sciences, Universidad Peruana de Ciencias Aplicadas, Lima, Peru; Laboratorio de Biología Molecular, Instituto de Investigación Nutricional, Lima, Peru. Electronic address: juana.delvalle@upc.pe.
Abstract
BACKGROUND: Huánuco is a central eastern region of Peru whose geography includes high forest and low jungle, as well as a mountain range that constitutes the inter-Andean valleys. It is considered a region endemic for dengue due to the many favorable conditions that facilitate transmission of the virus. METHODS: A total of 268 serum samples from patients in Huánuco, Peru with an acute febrile illness were assessed for the presence of dengue virus (DENV) via RT-PCR and NS1, IgM, and IgG ELISA during December 2015 and March 2016. RESULTS: DENV was detected in 25% of samples via RT-PCR, 19% of samples by NS1 antigen ELISA, and 10.5% of samples by IgM ELISA. DENV IgG was detected in 15.7% of samples by ELISA. The most frequent symptoms associated with fever across all groups were headache, myalgia, and arthralgia, with no significant difference between the four test methods CONCLUSIONS: In this study, DENV was identified in up to 25% of the samples using the standard laboratory method. In addition, a correlation was established between the frequency of positive results and the serological tests that determine NS1, IgM, and IgG. There is an increasing need for point-of-care tests to strengthen epidemiological surveillance in Peru.
BACKGROUND: Huánuco is a central eastern region of Peru whose geography includes high forest and low jungle, as well as a mountain range that constitutes the inter-Andean valleys. It is considered a region endemic for dengue due to the many favorable conditions that facilitate transmission of the virus. METHODS: A total of 268 serum samples from patients in Huánuco, Peru with an acute febrile illness were assessed for the presence of dengue virus (DENV) via RT-PCR and NS1, IgM, and IgG ELISA during December 2015 and March 2016. RESULTS:DENV was detected in 25% of samples via RT-PCR, 19% of samples by NS1 antigen ELISA, and 10.5% of samples by IgM ELISA. DENV IgG was detected in 15.7% of samples by ELISA. The most frequent symptoms associated with fever across all groups were headache, myalgia, and arthralgia, with no significant difference between the four test methods CONCLUSIONS: In this study, DENV was identified in up to 25% of the samples using the standard laboratory method. In addition, a correlation was established between the frequency of positive results and the serological tests that determine NS1, IgM, and IgG. There is an increasing need for point-of-care tests to strengthen epidemiological surveillance in Peru.
Authors: Juana Del Valle-Mendoza; Fernando Vasquez-Achaya; Miguel Angel Aguilar-Luis; Johanna Martins-Luna; Jorge Bazán-Mayra; Victor Zavaleta-Gavidia; Wilmer Silva-Caso; Hugo Carrillo-Ng; Yordi Tarazona-Castro; Ronald Aquino-Ortega; Luis J Del Valle Journal: BMC Res Notes Date: 2020-10-06