Cécile Le Saint1, Kimberley Crespo2, Amélie Bourdiec3, François Bissonnette4, Karen Buzaglo5, Bernard Couturier4, Sandra Bisotto3, Simon J Phillips6, Melissa Stutz7, Jean-Noël Gouze8, John S Sampalis7, Samir Hamamah9, Isaac Jacques Kadoch4. 1. Clinique Ovo, Ovo Labo, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada. Electronic address: c.lesaint@cliniqueovo.com. 2. Clinique Ovo, Ovo Labo, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada. Electronic address: k.crespo@cliniqueovo.com. 3. Clinique Ovo, Ovo R and D, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada. 4. Clinique Ovo, Ovo Labo, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada; Clinique Ovo, Ovo R and D, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada; Clinique Ovo, Ovo Fertility, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada; University of Montréal, Department of Obstetrics and Gynecology, Saint-Luc Hospital (CHUM), 1051 Sanguinet Street, Montréal Quebec H2 × 0C1, Canada. 5. Clinique Ovo, Ovo Fertility, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada; University of Montréal, Department of Obstetrics and Gynecology, Saint-Luc Hospital (CHUM), 1051 Sanguinet Street, Montréal Quebec H2 × 0C1, Canada. 6. Clinique Ovo, Ovo Fertility, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada; Faculty of Medicine, University of Montréal, Montréal Quebec, Canada. 7. JSS Research, 9400 Henri-Bourassa West, St-Laurent Quebec H4S 1N8, Canada. 8. Laboratoires Genévrier, 280 de Goa Street, ZI des Trois Moulins Antibes 06600, France. 9. ART/PGD Department, Arnaud-de-Villeneuve Hospital, University Hospital of Montpellier, Inserm U1203, CHRU Montpellier, Montpellier 34295, France.
Abstract
RESEARCH QUESTION: Does autologous endometrial cell co-culture (AECC) improve the number of good-quality blastocysts obtained by IVF/intracytoplasmic sperm injection (ICSI), compared with conventional embryo culture medium in a broad group of patients referred to assisted reproductive technology (ART)? DESIGN: This interventional, randomized, double-blind study took place at Clinique Ovo from March 2013 to October 2015 and included 207 healthy patients undergoing an IVF or ICSI protocol, of which 71 were excluded before randomization. On the previous cycle, all participants underwent an endometrial biopsy at D5 to D7 post-ovulation, following which the endometrial cells were prepared for AECC. RESULTS: The data demonstrated that AECC significantly increased the incidence of good-quality blastocysts compared with culture in conventional media (42.6% vs 28.4%, P < 0.001). No significant differences were found in pregnancy and live birth rates. CONCLUSION: This study demonstrated the benefits of AECC on blastocyst quality compared with conventional embryo culture medium, in a broader category of patients referred to ART as opposed to other studies that concentrated on specific causes of infertility only. However, limitations of the study design should be taken into consideration; the analysis was performed using embryos rather than patients and a follow-up of children born following the treatments could not be conducted.
RCT Entities:
RESEARCH QUESTION: Does autologous endometrial cell co-culture (AECC) improve the number of good-quality blastocysts obtained by IVF/intracytoplasmic sperm injection (ICSI), compared with conventional embryo culture medium in a broad group of patients referred to assisted reproductive technology (ART)? DESIGN: This interventional, randomized, double-blind study took place at Clinique Ovo from March 2013 to October 2015 and included 207 healthy patients undergoing an IVF or ICSI protocol, of which 71 were excluded before randomization. On the previous cycle, all participants underwent an endometrial biopsy at D5 to D7 post-ovulation, following which the endometrial cells were prepared for AECC. RESULTS: The data demonstrated that AECC significantly increased the incidence of good-quality blastocysts compared with culture in conventional media (42.6% vs 28.4%, P < 0.001). No significant differences were found in pregnancy and live birth rates. CONCLUSION: This study demonstrated the benefits of AECC on blastocyst quality compared with conventional embryo culture medium, in a broader category of patients referred to ART as opposed to other studies that concentrated on specific causes of infertility only. However, limitations of the study design should be taken into consideration; the analysis was performed using embryos rather than patients and a follow-up of children born following the treatments could not be conducted.
Authors: Ximo Garcia-Dominguez; Gianfranco Diretto; Sarah Frusciante; José Salvador Vicente; Francisco Marco-Jiménez Journal: Int J Mol Sci Date: 2020-09-26 Impact factor: 5.923