Steven Van Timmeren1, Ashfaq A Sial2, Srinivas K Lanka3, Nathan R Spaulding2, Rufus Isaacs1,4. 1. Department of Entomology, Michigan State University, East Lansing, MI, USA. 2. Department of Entomology, University of Georgia, Athens, GA, USA. 3. Department of Entomology, Kansas State University, Manhattan, KS, USA. 4. Ecology, Evolutionary Biology, and Behavior Program, Michigan State University, East Lansing, MI, USA.
Abstract
BACKGROUND: Spotted wing Drosophila is an invasive pest of fruit crops in most production regions globally, and insecticides are commonly used for its control. The biology of this pest combined with repeated pesticide exposure increases the risk of resistance to insecticides. We tested malathion, methomyl, spinetoram, spinosad, and zeta-cypermethrin against multiple colonies from each state using a contact bioassay method to determine diagnostic doses for assessment of insecticide susceptibility in this species. These were used to test populations collected in Michigan and Georgia, USA. RESULTS: Concentrations required to reach 50% (LC50 ) and 90% mortality (LC90 ) were calculated for the tested populations, and male mortality consistently occurred at lower concentrations than female mortality. Fly mortality did not vary significantly among populations collected from unmanaged, organic, and conventional fields. Similar results were found using the diagnostic concentrations applied to glass jars. CONCLUSIONS: Using this method, samples of D. suzukii that are freshly caught or reared from fruit can be tested within 1 day for their mortality in response to discriminating doses of five key insecticides. This method can be used to inform proactive resistance management strategies within integrated pest management programs.
BACKGROUND: Spotted wing Drosophila is an invasive pest of fruit crops in most production regions globally, and insecticides are commonly used for its control. The biology of this pest combined with repeated pesticide exposure increases the risk of resistance to insecticides. We tested malathion, methomyl, spinetoram, spinosad, and zeta-cypermethrin against multiple colonies from each state using a contact bioassay method to determine diagnostic doses for assessment of insecticide susceptibility in this species. These were used to test populations collected in Michigan and Georgia, USA. RESULTS: Concentrations required to reach 50% (LC50 ) and 90% mortality (LC90 ) were calculated for the tested populations, and male mortality consistently occurred at lower concentrations than female mortality. Fly mortality did not vary significantly among populations collected from unmanaged, organic, and conventional fields. Similar results were found using the diagnostic concentrations applied to glass jars. CONCLUSIONS: Using this method, samples of D. suzukii that are freshly caught or reared from fruit can be tested within 1 day for their mortality in response to discriminating doses of five key insecticides. This method can be used to inform proactive resistance management strategies within integrated pest management programs.
Authors: Michele Trombin de Souza; Mireli Trombin de Souza; Daniel Bernardi; Douglas José de Melo; Paulo Henrique Gorgatti Zarbin; Maria Aparecida Cassilha Zawadneak Journal: Sci Rep Date: 2021-02-17 Impact factor: 4.379
Authors: Michele Trombin de Souza; Mireli Trombin de Souza; Maíra Chagas Morais; Daiana da Costa Oliveira; Douglas José de Melo; Leonardo Figueiredo; Paulo Henrique Gorgatti Zarbin; Maria Aparecida Cassilha Zawadneak; Daniel Bernardi Journal: Molecules Date: 2022-09-21 Impact factor: 4.927