Establishment of a pulmonary epithelial barrier on biodegradable poly-L-lactic-acid membranes.

Development of biocompatible and functional scaffolds for tissue engineering is a major challenge, especially for development of polarised epithelia that are critical structures in tissue homeostasis. Different in vitro models of the lung epithelial barrier have been characterized using non-degradable polyethylene terephthalate membranes which limits their uses for tissue engineering. Although poly-L-lactic acid (PLLA) membranes are biodegradable, those prepared via conventional Diffusion Induced Phase Separation (DIPS) lack open-porous geometry and show limited permeability compromising their use for epithelial barrier studies. Here we used PLLA membranes prepared via a modification of the standard DIPS protocol to control the membrane surface morphology and permeability. These were bonded to cell culture inserts for use in barrier function studies. Pulmonary epithelial cells (H441) readily attached to the PLLA membranes and formed a confluent cell layer within two days. This was accompanied by a significant increase in trans-epithelial electrical resistance and correlated with the formation of tight junctions and vectorial cytokine secretion in response to TNFα. Our data suggest that a structurally polarized and functional epithelial barrier can be established on PLLA membranes produced via a non-standard DIPS protocol. Therefore, PLLA membranes have potential utility in lung tissue engineering applications requiring bio-absorbable membranes.

Introduction

The epithelial barrier of the skin, gastrointestinal and respiratory tract are the main interfaces between our body and the outside environment. Their function is to protect the body from environmental agents including pathogens and pollutants, dehydration, and heat loss. Moreover, the epithelial barriers are essential for the physiological functioning of tissues and organs permitting the formation and maintenance of tissue sub-compartments with different composition. Establishment of specialized cell adhesion complexes, especially tight junctions, are crucial to epithelial barrier integrity and function [1-3]. Conversely, the disruption of tight junction structure, due to specific mutations or altered regulatory signals can result in the development of a range of different diseases [4]. For example, in the lung, disruption of epithelial barrier function has been linked to asthma, COPD and cystic fibrosis [3, 5]. In the 2017 report from the Forum of International Respiratory Societies, respiratory diseases were highlighted as being among the principal causes of severe illness and death worldwide and consequently there is a great need for new and more effective treatments. Furthermore, since the lung epithelium offers a non-invasive and efficient route for the delivery of medical compounds, there is a critical need for methods and models in order to enable investigators to test drugs safety and effectiveness [6, 7].

Due to the complex interaction of genetic and environmental factors in the pathogenesis of human lung diseases, animal models have proved costly and relatively ineffective for lung research [6-8]. Although animal systems are still required by some authorities for pharmaceutical testing or toxicity evaluation, there has been a shift in focus from in vivo to ex vivo and in vitro models of the human lung epithelium [6]. Ex vivo models including whole lung perfusion, precision-cut lung slices and biopsy culture are useful models for the evaluation of immune and inflammatory responses as they maintain the in vivo tissue architecture and cellular composition, however, they are limited because of their short viability. Furthermore, with the possible exception of whole organ perfusion, exposure of excised tissue samples to challenge agents is not limited to an interaction with the epithelial barrier, as occurs in vivo. Different in vitro models of the lung epithelial barrier have been well characterized [6, 9]. These involve the use of either immortalised lung cell lines or primary cells from lung biopsy and represent better tools for the development of models that mimic normal or diseased epithelia [6]. However, these models tend to use non-biodegradable scaffolds, for example conventional Transwells with polyethylene terephthalate (PET) membranes, limiting their application in tissue engineering and regenerative medicine. For in vitro tissue engineering approaches, non-biodegradable scaffolds limit cell-cell interactions and are structurally different from naturally occurring extracellular matrix (ECM); in contrast biodegradable polymers can be replaced by naturally formed ECM overtime providing the cells with a more normal microenviroment. Extending this concept into regenerative medicine, non-biodegradable scaffolds are more likely to induce inflammatory reactions in the body whereas biodegradable constructs can aid tissue engraphment as they are replaced by naturally produced ECM and their degradation products are non-immunogenic. Therefore, there is a need for development of better support materials for epithelial cells that facilitate the establishment of a functional epithelial barrier while providing biodegradable and biocompatible scaffolds [9].

The development of functional and biocompatible scaffolds is a major challenge in biomedical engineering. Poly-L-lactic acid (PLLA) polymers are widely used for this purpose because of their biodegradability, mechanical properties, and most importantly because of their degradation rate, which is comparable to the healing time in a wound healing situation [10-12]. In particular, these polymers have been optimised for biomedical and pharmaceutical applications with particular focus on orthopaedics for tissue growth implants and fracture fixation devices, drug delivery systems, sutures and soft tissue repair [13, 14]. A conventional technique used for preparation of poly-L-lactic acid (PLLA) membrane is Diffusion Induced Phase Separation (DIPS) that has the advantage of generating membranes with specific characteristics (degradability, strength, morphology and thickness)[15]. However PLLA membranes produced using standard DIPS are only semi-porous, having a nonporous external surface reducing trans-membrane permeability which is critical for basolateral nutrient provision and polarised epithelial cell function [16-18]. Therefore, in order to improve the biocompatibility of PLLA membranes for epithelial cells, it is critical to control the surface characteristics of the membrane during the manufacturing steps. In our previous work [19] we developed a protocol for the preparation of PLLA membranes via a modification of the standard Diffusion Induced Phase Separation (DIPS) with sequential immersion into two coagulation baths. The double bath technique together with the regulation of coagulation bath composition and the desiccation conditions allowed the morphology of membrane surface to be controlled resulting in porous biodegradable PLLA membranes that are potentially more suitable for epithelial cell culture. In this work we demonstrated the formation of a functional lung epithelial barrier on PLLA membranes with optimised ultrastructure and surface characteristics. These may ultimately have application in bioengineering and regenerative medicine.

Materials and methods

PLLA membranes

PLLA membranes were made using the poly-L-lactic acid polymer RESOMER L 209 S (Boehringer-Ingelheim, Berkshire, UK) with an inherent viscosity of 3 dl/g using a modification of the standard DIPS method, as previously described [20]. In this work, membranes were prepared via sequential immersion into two coagulation baths. The solvents employed were deionized water and 1,4 dioxane (Sigma-Aldrich, Poole UK). The composition of first coagulation bath was 87:13 dioxane:water (wt:wt) while the second coagulation bath was pure water. The soaking times in the first and second coagulation baths were maintained constant at 5 minutes. After the DIPS process, the resultant membranes were dehydrated in an environment with about 70% relative humidity for 24 h. The surface morphology of the membranes was assessed by scanning electron microscopy (Philips SEM Quanta, FEI) after gold coating to make them conductive. PET membranes were removed from commercial cell culture inserts which were then used as supports for the PLLA membranes. PLLA membranes were cut to size and attached to the base of the cell culture insert by bonding with a biocompatible silicone adhesive (SIMTEC silicone parts, Florida, USA) which was applied to the base the cell culture insert to create a cell culture well. When the adhesive had dried, the entire PLLA culture insert was sterilized using ethanol 70%v/v and dried before use.

Cell culture and stimulation

NCl-H441 (American Type Culture Collection, HTB-174) cells were obtained from LGC Standards (Teddington, UK) and cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), 1mM sodium pyruvate, 100 U/mL penicillin, and 100 μg/mL streptomycin (all from Fisher Scientific-UK Ltd, Loughborough, UK) at 37 °C in a humidified air atmosphere containing 5% CO2. For establishment of an epithelial barrier, the H441 cells (1.5*105 cells/insert) were cultured on the apical side of cell culture inserts in the presence of dexamethasone (Sigma-Aldrich, Poole, UK) with 1% insulin-transferrin-sodium selenite (ITS) supplement (Roche Diagnostics Limited, West Sussex, UK). The membrane supports of the cell culture inserts (0.33 cm2 cell culture area) were either the PLLA polymer or PET (Transwell Clear inserts (pore size 0.4 μm), Corning, VWR, Dublin, Ireland). Responses of H441 cells to the pro-inflammatory cytokine TNFα (10ng/ml; Sigma-Aldrich, Poole, UK) were assessed on day 4 post-seeding by challenging the apical epithelial surface with the cytokine or vehicle control; after 24h the cell-free culture supernatants from the apical and basal compartments were collected and stored for ELISA, while the cells were fixed for immunofluorescence staining.

Fluorescent labelling of cells

CellVue Jade Cell Labeling Kit (Fisher Scientific-UK Ltd, Loughborough, UK) was used to evaluate cell monolayer formation on the PLLA membranes. H441 cells were cultured on PLLA cell culture inserts for 48h, washed with PBS and cell membranes were stained using CellVue Jade Cell Labeling Kit. Cell nuclei were counterstained with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) (1:1000 dilution; Merck Millipore, Darmstadt, Germany). Fluorescent images were acquired using a fluorescence microscope Leica DMI 6000B (Leica Microsystem, Milton Keynes, United Kingdom).

Hematoxylin and eosin (H&E) staining

H441 cells were cultured in PLLA cell culture inserts for 48h, fixed with 4% formaldehyde (Taab Laboratory Equipment Ltd, Reading, UK) and the excised membranes embedded in paraffin (Leica Microsystems (UK) Ltd, Milton Keynes, UK). H&E staining was performed using a Shandon Varistain 24–4 automatic slide stainer (Fisher Scientific-UK Ltd, Loughborough, UK) on 6 μm sections of the cell-covered membranes.

Bioelectrical measurements

Transepithelial electrical resistance (TER) was measured daily using STX01 electrodes connected to a Millicell ERS-2 volt-ohm meter (Merck Millipore, Darmstadt, Germany). TER readings (ohms) were corrected for the background value obtained using a PLLA or PET (TER = TER(cell layer)-TER(empty insert)) cell culture insert containing growth medium alone and then adjusted for the area of the insert (ohms*cm2). TER was measured daily after culturing H441 cells in the absence or presence of dexamethasone to assess the formation of a functional epithelial barrier by measuring the electrical resistance.

Immunofluorescence staining

H441 cells were cultured on PLLA and PET cell culture inserts for 5 days and then fixed with 4% paraformaldehyde followed by permeabilization and staining with occludin-conjugated-Alexa Fluor 488 fluorescent antibody (Fisher Scientific-UK Ltd, Loughborough, UK) (1:100) for the detection of tight junctions [21]. Cell nuclei were counterstained with DAPI. Fluorescent images were acquired using a fluorescence microscope Leica DMI 6000B (Leica Microsystem, Milton Keynes, United Kingdom).

ELISA analysis

Human Interleukin 8 (IL-8) was evaluated in culture media from the apical and basolateral chambers using an IL-8 DuoSet ELISA (R&D, Abingdon, UK) in accord with manufacturer’s instructions. Each sample was evaluated using 2 technical replicates and the mean value used for subsequent analyses.

Statistics

Results are expressed as means of ± SD. Differences between groups were assessed using an unpaired T-test or 2way ANOVA for multiple comparisons. All data were analyzed using Prism (GraphPad, CA, USA). p<0.05 was accepted as statistically significant. *p<0.05, **p<0.01, ***p<0.001.

Results

Evaluation of cellular monolayer formation on PLLA membranes prepared using a modification of the standard DIPS technique

By using a modification of the standard DIPS method, we produced PLLA membranes with high porosity and permeability characteristics that would be suitable for epithelial barrier studies. Initially, we optimized H441 epithelial cell growth on the PLLA membranes in order to assess the formation of a uniform cell monolayer. The cells were cultured on the upper surface of the PLLA membranes (Fig 1A and 1B) within the cell culture inserts and after 48h they had formed a confluent and homogeneous cell monolayer (Fig 1C and 1D).

Fig 1

Cell layer formation on PLLA cell culture inserts.

A) Morphology of the upper PLLA membrane surface obtained via modification of the standard DIPS method. B) Schematic representation of PLLA cell culture insert. C) An H441 cell monolayer was grown in a PLLA membrane within a cell culture insert and labelled with the fluorescent cell membrane tracker (Blue DAPI and Green Dye-Cell tracker). D) H&E staining of a section of the PLLA membrane covered with an epithelial monolayer after 48 h culture in a cell culture insert.

Comparison of PLLA and PET membranes on epithelial paracellular ionic permeability

Measurement of transepithelial electrical resistance (TER) is used to assess the formation of a functional epithelial barrier on porous membranes where TER reflects the formation of tight junctions and regulation of paracellular ionic permeability across the cell layer [22]. It has previously been shown that H441 cells need dexamethasone for the establishment of a proper epithelial barrier [23]. Therefore, we performed a dose response and time course study of barrier formation in absence and presence of dexamethasone to establish the minimum dose required for maximal polarization while minimising anti-inflammatory effects of the corticosteroid. This showed that after 48h we were able to detect a significant increase in TER in presence of dexamethasone using either PLLA (Fig 2A) or PET (Fig 2B) membranes. Our results showed similar increases in TER for the PLLA membranes compared with PET membranes over both time and doses of dexamethasone tested. Maximal TER values were achieved with Dexamethasone at concentrations > 20 nM. Therefore, we continued our experiments using 20 and 40 nM.

Fig 2

Dexamethasone dose response and time course for H441 barrier formation on PLLA (A) or PET (B) membranes.

TER values are expressed as fold change compared to the control (no dexamethasone) on each day (dashed line); maximum TER values were in the range of 359–563 ohms*cm2 for PLLA and 455–721 ohms*cm2 for PET membranes. TER was calculated as ohms*cm2 and corrected for the background value detected in an empty cell culture insert containing medium alone (PET = 54.5 ohms*cm2 and PLLA = 99 ohms*cm2). Data are mean ± SD, n = 3 independent experiments each performed in duplicate.

Formation of tight junctions between epithelial cells cultured on PLLA and PET membranes

Cell-cell adhesive interactions and formation of tight junctions is essential for control of paracellular ionic permeability. Therefore, we performed immunofluorescent staining of H441 cells cultured on PLLA or PET membranes to detect occludin, one of the main protein components of tight junctions. For cells cultured in the presence of dexamethasone, the results show clear formation of tight junctions between the cells with a distinct and continuous distribution around the perimeter of each cell (Fig 3), while in absence of dexamethasone the occludin staining was irregular and discontinuous, consistent with the lower TER readings. The formation of tight junctions, as detected by occludin staining, was comparable between PLLA and PET membranes (Fig 3), although it appeared that the cell layer was flatter and more regular on the PLLA membrane perhaps due to their better biomechanical properties.

Fig 3

Immunofluorescent images showing tight junctions immunostained using an anti-occludin 488 Alexa Fluor conjugated antibody.

Cells were cultured for 5 days on PLLA (upper images) or PET (lower images) membranes without or with dexamethasone treatment (20 and 40 nM). Data are representative of 2 independent experiments.

Polarised cytokine release in response to the proinflammatory cytokine TNFα

To further investigate the characteristics of the epithelial monolayer on PLLA membranes, we evaluated the response and functionality of polarised H441 cultures in presence of an inflammatory stimulus. We chose TNFα as it is able to increase epithelial barrier ionic permeability (ie. decrease TER) and induce the release of other inflammatory factors including the chemokine, interleukin 8 (IL-8). Therefore, we established polarised epithelial cell layers on PLLA or PET membranes by 72h of culture with dexamethasone before exposing the apical epithelial surface to TNFα (10ng/ml). This concentration was chosen because it causes the modulation of tight junctions, and can stimulate cytokine release [9]. After 24h of exposure to TNFα, there was a significant decrease in TER compared to untreated controls using H441 cells cultured on either PLLA or PET membranes (Fig 4A) and the increase in epithelial barrier ionic permeability was similar using either type of membrane. Moreover, TNFα was also able to stimulate the release of its downstream mediator [24], IL-8 into both the apical (Fig 4B) and basolateral compartments with no significant difference between cells cultured on either PLLA or PET membranes.

Fig 4

Comparison of the effect of TNFα on polarised epithelial cell layers cultures on PLLA or PET membranes.

(A) TER percentage change after 24h treatment with TNFα. (B, C) IL-8 released into apical (B) or basolateral (C) conditioned media of cultures. Data are mean ± SD, n = 3 independent experiments each performed in duplicate.

Discussion and conclusions

Development of biomaterials suitable for both in vivo and in vitro tissue engineering applications offers opportunities not only for regenerative medicine, but also for target discovery, preclinical evaluation, drug transport and toxicology studies. In our work we show that a functional epithelial barrier can be established on biodegradable PLLA membranes that have enhanced surface porosity and permeability through use of a modification of the standard DIPS protocol. Our results show that the epithelial barrier properties are comparable to those using more conventional PET membranes, however PLLA may be a more suitable material for development of more complex tissue mimetic models because of its biodegradable properties and the possibility of controlling membrane structure. Moreover, PLLA polymers, which are Federal Drug Administration (FDA)-approved, offer an excellent biocompatible and biodegradable scaffold for tissue engraftment in vivo where, after its initial role as a cellular support, it can be degraded by physiological processes. This is especially relevant for pulmonary patients with permanent damage, stenosis or a tumour in the trachea; these patients have a poor quality of life because only limited reconstruction options are currently available. However, tissue engineering and regenerative medicine in the lung offers considerable potential [25] and will require establishment of an effective epithelial tissue barrier.

Many techniques have been reported for fabricating porous PLLA membranes. Some of these include methods such as air spinning [26], solvent-cast/particulate leaching [27] and phase separation [16]. Among these techniques, the phase separation approach is one of the most important for obtaining porous structures; such approaches include thermally induced phase separation, air-casting of polymer solution, precipitation from vapour phase, and immersion precipitation [28]. However, DIPS via immersion precipitation is the most widely used membrane preparation method and is an efficient method for the preparation of PLLA scaffolds [16, 29]. Unfortunately, PLLA membranes prepared via conventional DIPS show a non-porous external surface reducing overall membrane permeability and limiting potential applications of PLLA membranes for barrier permeability studies. However, using a modification of the DIPS technique, PLLA membranes can be prepared that allow the morphology of membrane surface to be controlled, resulting in fabrication of porous biodegradable PLLA membranes [19]. Here we show that these membranes can support the growth and polarization of airway epithelial cells. Furthermore, these cultures are functional as shown by regulation of paracellular ionic permeability and vectorial cytokine release in response to TNFα.

The epithelial barrier in the lung is a very dynamic structure that responds to both physiological and pathological stimuli. Its barrier function is achieved by expression of intercellular adhesion complexes, including tight junctions that determine apical-basolateral polarity and control the passage of ions and macromolecules across the lung epithelium [3, 30, 31]. The epithelium also contributes to innate immunity through vectorial release of cytokines and chemokines that signal to cells of the innate and adaptive immune system [32-35]. In our work we successfully established a functional epithelial barrier on a PLLA scaffold prepared using a modified DIPS protocol. We observed the formation of cellular tight junctions resulting in an electrically tight layer of epithelial cells. Moreover, we observed vectorial release of the neutrophil chemokine, IL-8, in response to TNFα. While other studies have tested the utility of PLLA membranes, these have usually been limited to demonstration of their ability to support growth of human cells, including respiratory epithelial cells [36], to date, there has been little functional assessment of biological barrier properties [26, 27, 37]. For example, Selvam et al. used a solvent-cast/particulate leaching technique to fabricate microporous PLLA membranes from PLLA/polyethylene glycol blends and confirmed permeation of glucose, L-tryptophan, and dextran, as well as growth of lacrimal acinar cells which retained histiotypic morphological and physiological characteristics of in vivo. However, there was no direct assessment of epithelial barrier formation and function. Similarly, Zhu et al. demonstrated that endothelial cells attach, spread and grow on PLLA membranes modified by immobilization of chitosan, chondroitin sulfate and collagen type I, however they did not study endothelial barrier formation or vectorial responses. This contrasts with our own studies in which we show establishment of tight junctions and an electrically tight ionic barrier. Furthermore, the cellular barrier was able to respond and adapt the physiological stimulus, TNFα, consistent with previous studies using PET membranes [38].

The minimal morphological and structural characteristics of an ideal scaffold for epithelial barrier tissues include offering a support for cell attachment, growth and polarisation, and providing adequate permeability to allow proper exchange of nutrients. However, PLLA scaffolds can offer much more: they can be produced with different morphologies, porosity, surface modifications and degradation times which can be optimised for the cell types under investigation. Furthermore, the microporous nature of PLLA scaffolds can be exploited for infiltration and colonization by mesenchymal cells that synthesise extracellular matrix (ECM) deposition and produce factors that support the barrier tissues. The accumulation of natural ECM while the PLLA membrane gradually dissolves will eventually leave the epithelial cells on a physiologically optimal substrate. Just as epithelial cells provide a barrier to the external environment, endothelial cells form a barrier with the blood compartment, so PLLA scaffolds may also be useful for endothelial barriers studies. In this case, the porosity and biodegradable nature of the scaffold may also facilitate movement of different types of cells that normally circulate in the blood compartment (leukocytes, platelets) into the tissue construct [21, 39, 40]. Thus, ultimately biodegradable PLLA scaffolds may be used to create complex constructs that more closely recapitulate the in vivo tissue environment.

In conclusion, we have used a modification of the standard DIPS protocol to control the membrane surface morphology and permeability of PLLA membranes and demonstrated that they support a functional lung epithelial barrier that dynamically responds to the proinflammatory stimulus, TNFα. Since loss of barrier function has been implicated in respiratory disease like asthma, chronic obstructive pulmonary disease (COPD), acute lung injury (ALI) and fibrosis [2, 4, 30], the results obtained in our work provide new opportunities for the application of PLLA membranes for tissue engineering for target discovery and preclinical testing.

Transepithelial electrical resistance (TER) during time course/dose response experiment.

The TER values are the mean of 2 reading in 2 different inserts calculated as ohms*cm2 and corrected for the background value detected in an empty cell culture insert containing medium alone (PET = 54.5 ohms*cm2 and PLLA = 99 ohms*cm2).

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IL8 ELISA reading on H441 conditioned media in absence or presence of TNFalpha.

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