Fatih Cakir1, Mutalip Cicek2, Ibrahim Halil Yildirim3. 1. Department of Microbiology, Faculty of Medicine, Dicle University, Diyarbakir, Turkey. sfcakir@gmail.com. 2. Department of Microbiology, Faculty of Medicine, Ahi Evran University, Kirsehir, Turkey. 3. Department of Genetics, Faculty of Veterinary, Dicle University, Diyarbakir, Turkey.
Abstract
PURPOSE: The present study aimed to determine the frequency of subtypes of Blastocystis sp. in the immunosuppressed individuals, in patients with chronic urticaria, and in patients with GIS complaints to investigate the difference of Blastocystis sp. subtype distribution between patient and control groups. METHODS: A total of 345 stool samples were collected from the patients and samples were studied by native-Lugol, trichrome staining, and Jones medium culture method. Positively detected samples were subjected to PCR to determine the subtypes. RESULTS: This is the first study of nine subtypes of Blastocystis sp. investigated in our country and the most frequently found subtype was ST3, and then, the other subtypes were ST1, ST2, ST5, and ST6, respectively. Mix subtype was detected in the 11.6% and no subtype was detected in the 17.4% of the samples. The ST5 was detected first time in the control group and ST6, which is reported limitedly in our country, was found in patients with GIS complaints. ST1 and ST2 were found higher in the patient group. CONCLUSION: This study confirmed that the subtype (ST) differences are an important factor affecting the pathogenesis of Blastocystis sp.
PURPOSE: The present study aimed to determine the frequency of subtypes of Blastocystis sp. in the immunosuppressed individuals, in patients with chronic urticaria, and in patients with GIS complaints to investigate the difference of Blastocystis sp. subtype distribution between patient and control groups. METHODS: A total of 345 stool samples were collected from the patients and samples were studied by native-Lugol, trichrome staining, and Jones medium culture method. Positively detected samples were subjected to PCR to determine the subtypes. RESULTS: This is the first study of nine subtypes of Blastocystis sp. investigated in our country and the most frequently found subtype was ST3, and then, the other subtypes were ST1, ST2, ST5, and ST6, respectively. Mix subtype was detected in the 11.6% and no subtype was detected in the 17.4% of the samples. The ST5 was detected first time in the control group and ST6, which is reported limitedly in our country, was found in patients with GIS complaints. ST1 and ST2 were found higher in the patient group. CONCLUSION: This study confirmed that the subtype (ST) differences are an important factor affecting the pathogenesis of Blastocystis sp.
Entities:
Keywords:
Blastocystis sp.; Pathogenicity; Polymerase chain reaction; Subtypes of Blastocystis
Authors: Shaimaa H Ali; Mousa A M Ismail; Ayman A El-Badry; Enas Y Abu-Sarea; Ahmad M Dewidar; Doaa A Hamdy Journal: Acta Parasitol Date: 2022-01-24 Impact factor: 1.534