| Literature DB >> 30649573 |
Xiaoman You1, Wenwei Zhang1, Jinlong Hu1, Ruonan Jing1, Yue Cai1, Zhiming Feng1, Fei Kong1, Jie Zhang1, Haigang Yan1, Weiwei Chen2, Xingang Chen1, Jing Ma1, Xiaojie Tang1, Peng Wang1, Shanshan Zhu2, Linglong Liu1, Ling Jiang1, Jianmin Wan3,4.
Abstract
KEY MESSAGE: FLO15encodes a plastidic glyoxalase I protein, OsGLYI7, which affects compound starch granule formation and starch synthesis in rice endosperm. Starch synthesis in rice (Oryza sativa) endosperm is a sophisticated process, and its underlying molecular machinery still remains to be elucidated. Here, we identified and characterized two allelic rice floury endosperm 15 (flo15) mutants, both with a white-core endosperm. The flo15 grains were characterized by defects in compound starch granule development, along with decreased starch content. Map-based cloning of the flo15 mutants identified mutations in OsGLYI7, which encodes a glyoxalase I (GLYI) involved in methylglyoxal (MG) detoxification. The mutations of FLO15/OsGLYI7 resulted in increased MG content in flo15 developing endosperms. FLO15/OsGLYI7 localizes to the plastids, and the in vitro GLYI activity derived from flo15 was significantly decreased relative to the wild type. Moreover, the expression of starch synthesis-related genes was obviously altered in the flo15 mutants. These findings suggest that FLO15 plays an important role in compound starch granule formation and starch synthesis in rice endosperm.Entities:
Keywords: ENDOSPERM 15; FLOURY; Glyoxalase I; Rice·endosperm; Starch synthesis·compound granule
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Year: 2019 PMID： 30649573 DOI： 10.1007/s00299-019-02370-9
Source DB: PubMed Journal: Plant Cell Rep ISSN： 0721-7714 Impact factor: 4.570