Liyun Wang1, Xingyu Yang1, Chunjun Yang1, Jing Gao1, Ye Zhao2, Cheng Cheng3, Guoping Zhao4, Shengxiu Liu5. 1. Department of Dermatology, The Second Affiliated Hospital, Anhui Medical University, Hefei, Anhui, PR China. 2. Teaching & Research Section of Nuclear Medicine, Anhui Medical University, Hefei, Anhui, PR China. 3. The Institute of Plasma Physics, Chinese Academy of Science, Hefei, Anhui, PR China. 4. Anhui Province Key Laboratory of Environmental Toxicology & Pollution Control Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, PR China. 5. Department of Dermatology, The First Affiliated Hospital, Anhui Medical University, Hefei, Anhui, PR China.
Abstract
AIM: This study investigated the effect and mechanism of cold atmospheric plasma (CAP)-activated media on A431 and HaCaT cells. MATERIALS & METHODS: Phosphate-buffered saline (PBS) and Dulbecco's Modified Eagle's Medium (DMEM) were treated by CAP to get CAP-activated media. A431 and HaCaT were incubated by CAP-activated media for 2 h. MTT, Annexin-V and propidium iodide (PI), Western blot and reactive oxygen species (ROS) detection assay were utilized to demonstrate the effect. RESULTS: The CAP-activated media decreased the proliferation of A431 cells in a dose/time-dependent manner. And the CAP-activated media could induce apoptosis in A431 cells. CAP-activated phosphate-buffered saline could increase intracellular ROS level but not CAP-activated DMEM. CONCLUSION: CAP-activated media could induce apoptosis in A431 cells by production of ROS.
AIM: This study investigated the effect and mechanism of cold atmospheric plasma (CAP)-activated media on A431 and HaCaT cells. MATERIALS & METHODS:Phosphate-buffered saline (PBS) and Dulbecco's Modified Eagle's Medium (DMEM) were treated by CAP to get CAP-activated media. A431 and HaCaT were incubated by CAP-activated media for 2 h. MTT, Annexin-V and propidium iodide (PI), Western blot and reactive oxygen species (ROS) detection assay were utilized to demonstrate the effect. RESULTS: The CAP-activated media decreased the proliferation of A431 cells in a dose/time-dependent manner. And the CAP-activated media could induce apoptosis in A431 cells. CAP-activated phosphate-buffered saline could increase intracellular ROS level but not CAP-activated DMEM. CONCLUSION: CAP-activated media could induce apoptosis in A431 cells by production of ROS.
Authors: Henrike Rebl; Marie Sawade; Martin Hein; Claudia Bergemann; Manuela Wende; Michael Lalk; Peter Langer; Steffen Emmert; Barbara Nebe Journal: Sci Rep Date: 2022-02-15 Impact factor: 4.379
Authors: Pavol Zubor; Yun Wang; Alena Liskova; Marek Samec; Lenka Koklesova; Zuzana Dankova; Anne Dørum; Karol Kajo; Dana Dvorska; Vincent Lucansky; Bibiana Malicherova; Ivana Kasubova; Jan Bujnak; Milos Mlyncek; Carlos Alberto Dussan; Peter Kubatka; Dietrich Büsselberg; Olga Golubnitschaja Journal: Int J Mol Sci Date: 2020-10-27 Impact factor: 5.923