Didem Pekmezci1, Gokmen Zafer Pekmezci2, Alparslan Yildirim3, Onder Duzlu3, Abdullah Inci3. 1. Department of Internal Medicine, Faculty of Veterinary Medicine, University of Ondokuz Mayis, 55200, Samsun, Turkey. dkazanci@omu.edu.tr. 2. Preclinical Sciences, Faculty of Veterinary Medicine, University of Ondokuz Mayis, 55200, Samsun, Turkey. 3. Department of Parasitology, Faculty of Veterinary Medicine, University of Erciyes, 38039, Kayseri, Turkey.
Abstract
OBJECTIVE: This preliminary study was conducted to reveal that the molecular identity of Encephalitozoon spp. and Enterocytozoon bieneusi in indoor domestic cats' fecal samples from Turkey was screened using the PCR. MATERIALS AND METHODS: Nested PCR was performed using MSP and EBITS primers. All of the amplification products were sequenced to identify the microsporidia species. RESULTS: Four (5.5%) and three (4.1%) genomic DNA isolates of the fecal samples from 72 indoor domestic cats showed amplification of the ITS regions of E. bieneusi and Encephalitozoon spp., respectively. Two different genotypes, D and IV, of E. bieneusi were determined in two cats each based on the ITS sequence analyses. Moreover, Encephalitozoon spp. sequence analyses revealed three isolates belonging to E. intestinalis. CONCLUSIONS: This preliminary study has provided the first molecular data on the zoonotic genotypes of E. bieneusi and E. intestinalis in cats in Turkey. Furthermore, E. bieneusi genotype IV (accession number MG727664) was submitted to GenBank for the first time in the Western Palearctic Region as hosted by a domestic cat. Additionally, E. intestinalis (accession number MG570080) was also submitted to GenBank as a valid ITS sequence for the first time as hosted by a domestic cat worldwide.
OBJECTIVE: This preliminary study was conducted to reveal that the molecular identity of Encephalitozoon spp. and Enterocytozoon bieneusi in indoor domestic cats' fecal samples from Turkey was screened using the PCR. MATERIALS AND METHODS: Nested PCR was performed using MSP and EBITS primers. All of the amplification products were sequenced to identify the microsporidia species. RESULTS: Four (5.5%) and three (4.1%) genomic DNA isolates of the fecal samples from 72 indoor domestic cats showed amplification of the ITS regions of E. bieneusi and Encephalitozoon spp., respectively. Two different genotypes, D and IV, of E. bieneusi were determined in two cats each based on the ITS sequence analyses. Moreover, Encephalitozoon spp. sequence analyses revealed three isolates belonging to E. intestinalis. CONCLUSIONS: This preliminary study has provided the first molecular data on the zoonotic genotypes of E. bieneusi and E. intestinalis in cats in Turkey. Furthermore, E. bieneusi genotype IV (accession number MG727664) was submitted to GenBank for the first time in the Western Palearctic Region as hosted by a domestic cat. Additionally, E. intestinalis (accession number MG570080) was also submitted to GenBank as a valid ITS sequence for the first time as hosted by a domestic cat worldwide.
Authors: B Dengjel; M Zahler; W Hermanns; K Heinritzi; T Spillmann; A Thomschke; T Löscher; R Gothe; H Rinder Journal: J Clin Microbiol Date: 2001-12 Impact factor: 5.948
Authors: Alejandro Dashti; Mónica Santín; Lourdes Cano; Aida de Lucio; Begoña Bailo; Marta Hernández de Mingo; Pamela C Köster; José A Fernández-Basterra; Juan Aramburu-Aguirre; Nuria López-Molina; Juan C Fernández-Crespo; Rafael Calero-Bernal; David Carmena Journal: Parasitol Res Date: 2019-08-21 Impact factor: 2.289