| Literature DB >> 30643212 |
Marco Castello1, Giorgio Tortarolo1,2, Mauro Buttafava3, Takahiro Deguchi4, Federica Villa3, Sami Koho1, Luca Pesce4, Michele Oneto4, Simone Pelicci4, Luca Lanzanó4, Paolo Bianchini4, Colin J R Sheppard4, Alberto Diaspro4,5, Alberto Tosi3, Giuseppe Vicidomini6.
Abstract
Image scanning microscopy (ISM) can improve the effective spatial resolution of confocal microscopy to its theoretical limit. However, current implementations are not robust or versatile, and are incompatible with fluorescence lifetime imaging (FLIM). We describe an implementation of ISM based on a single-photon detector array that enables super-resolution FLIM and improves multicolor, live-cell and in-depth imaging, thereby paving the way for a massive transition from confocal microscopy to ISM.Mesh：
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Year: 2019 PMID： 30643212 DOI： 10.1038/s41592-018-0291-9
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547