Roshan Kumar Mahat1, Neelima Singh2, Vedika Rathore3, Manisha Arora4, Tapeshwar Yadav5. 1. Department of Biochemistry, Gajra Raja Medical College, Jiwaji University, Gwalior, Madhya Pradesh, 474009, India. Electronic address: mahatroshan79@gmail.com. 2. University of Kota, Kota, Rajasthan, 324005, India. 3. Department of Biochemistry, Shyam Shah Medical College, Rewa, Madhya Pradesh, 486001, India. 4. Department of Biochemistry, Muzaffarnagar Medical College, Muzaffarnagar, Uttar Pradesh, 251203, India. 5. Department of Laboratory Medicine, Green Tara College of Health Sciences, Bhaisepati, Lalitpur, Nepal.
Abstract
BACKGROUND: Prediabetes is a condition in which blood glucose level is above the normal but below the diagnostic value of diabetes mellitus. Besides progression to diabetes mellitus, prediabetic subjects are at risk for cardiovascular disease (CVD). It is associated with oxidative stress and inflammation and therefore this research was conducted with the aim to evaluate the risk of cardiovascular disease in prediabetic subjects by measuring the markers of oxidative stress and inflammation and their possible correlation with glucose intolerance. MATERIALS AND METHODS: A total of 400 human subjects were recruited for the present cross-sectional study. Of them, 200 were prediabetic subjects and 200 were age and gender-matched control subjects. Blood samples were collected from all participants and analyzed for 8-hydroxy-2'-deoxy-guanosine (8-OHdG), malondialdehyde (MDA), reduced glutathione (GSH) and high sensitivity C-reactive protein (hs-CRP). RESULTS: The markers of oxidative stress i.e. 8-OHdG and MDA were found to be significantly increased in prediabetic subjects as compared to control subjects except GSH, which was significantly reduced in prediabetic subjects. Similarly, hs-CRP (a marker of inflammation) was significantly increased in prediabetic subjects compared to controls. On correlation analysis, 8-OHdG, MDA and hs-CRP were significantly and positively correlated with glucose intolerance in prediabetes whereas GSH showed significant negative correlation with glucose intolerance. CONCLUSION: In conclusion, markers of oxidative stress and inflammation should be taken into consideration while evaluating the risk for CVD in prediabetes since these markers were well correlated with glucose intolerance in prediabetic subjects.
BACKGROUND: Prediabetes is a condition in which blood glucose level is above the normal but below the diagnostic value of diabetes mellitus. Besides progression to diabetes mellitus, prediabetic subjects are at risk for cardiovascular disease (CVD). It is associated with oxidative stress and inflammation and therefore this research was conducted with the aim to evaluate the risk of cardiovascular disease in prediabetic subjects by measuring the markers of oxidative stress and inflammation and their possible correlation with glucose intolerance. MATERIALS AND METHODS: A total of 400 human subjects were recruited for the present cross-sectional study. Of them, 200 were prediabetic subjects and 200 were age and gender-matched control subjects. Blood samples were collected from all participants and analyzed for 8-hydroxy-2'-deoxy-guanosine (8-OHdG), malondialdehyde (MDA), reduced glutathione (GSH) and high sensitivity C-reactive protein (hs-CRP). RESULTS: The markers of oxidative stress i.e. 8-OHdG and MDA were found to be significantly increased in prediabetic subjects as compared to control subjects except GSH, which was significantly reduced in prediabetic subjects. Similarly, hs-CRP (a marker of inflammation) was significantly increased in prediabetic subjects compared to controls. On correlation analysis, 8-OHdG, MDA and hs-CRP were significantly and positively correlated with glucose intolerance in prediabetes whereas GSH showed significant negative correlation with glucose intolerance. CONCLUSION: In conclusion, markers of oxidative stress and inflammation should be taken into consideration while evaluating the risk for CVD in prediabetes since these markers were well correlated with glucose intolerance in prediabetic subjects.
Authors: Md Raihan Uzzaman Prince; S M Neamul Kabir Zihad; Puja Ghosh; Nazifa Sifat; Razina Rouf; Gazi Mohammad Al Shajib; Md Ashraful Alam; Jamil A Shilpi; Shaikh J Uddin Journal: Front Nutr Date: 2021-05-10