| Literature DB >> 3063606 |
S Chandrasegaran1, K D Lunnen, H O Smith, G G Wilson.
Abstract
The HinfI restriction and modification genes were cloned on a 3.9-kb PstI fragment inserted into the PstI site of plasmid pBR322. Both genes are confined to an internal 2.3-kb BclI-AvaI subfragment. This subfragment was sequenced. Two large open reading frames (ORF's) are present. ORF1 codes for the methylase [predicted 359 amino acids (aa)] and ORF2 codes for the endonuclease (predicted 262 or 272 aa).Entities:
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Year: 1988 PMID: 3063606 DOI: 10.1016/0378-1119(88)90210-7
Source DB: PubMed Journal: Gene ISSN: 0378-1119 Impact factor: 3.688